Pro-inflammatory mediation of myoblast proliferation.

Skeletal muscle satellite cell function is largely dictated by the surrounding environment following injury. Immune cell infiltration dominates the extracellular space in the injured area, resulting in increased cytokine concentrations. While increased pro-inflammatory cytokine expression has been p...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jeffrey S Otis, Sarah Niccoli, Nicole Hawdon, Jessica L Sarvas, Melinda A Frye, Adam J Chicco, Simon J Lees
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b0fca2be516c48079d5a5151485b47dc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b0fca2be516c48079d5a5151485b47dc
record_format	dspace
spelling	oai:doaj.org-article:b0fca2be516c48079d5a5151485b47dc2021-11-18T08:27:13ZPro-inflammatory mediation of myoblast proliferation.1932-620310.1371/journal.pone.0092363https://doaj.org/article/b0fca2be516c48079d5a5151485b47dc2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24647690/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Skeletal muscle satellite cell function is largely dictated by the surrounding environment following injury. Immune cell infiltration dominates the extracellular space in the injured area, resulting in increased cytokine concentrations. While increased pro-inflammatory cytokine expression has been previously established in the first 3 days following injury, less is known about the time course of cytokine expression and the specific mechanisms of cytokine induced myoblast function. Therefore, the expression of IL-1β and IL-6 at several time points following injury, and their effects on myoblast proliferation, were examined. In order to do this, skeletal muscle was injured using barium chloride in mice and tissue was collected 1, 5, 10, and 28 days following injury. Mechanisms of cytokine induced proliferation were determined in cell culture using both primary and C2C12 myoblasts. It was found that there is a ∼20-fold increase in IL-1β (p≤0.05) and IL-6 (p = 0.06) expression 5 days following injury. IL-1β increased proliferation of both primary and C2C12 cells ∼25%. IL-1β stimulation also resulted in increased NF-κB activity, likely contributing to the increased proliferation. These data demonstrate for the first time that IL-1β alone can increase the mitogenic activity of primary skeletal muscle satellite cells and offer insight into the mechanisms dictating satellite cell function following injury.Jeffrey S OtisSarah NiccoliNicole HawdonJessica L SarvasMelinda A FryeAdam J ChiccoSimon J LeesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92363 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeffrey S Otis Sarah Niccoli Nicole Hawdon Jessica L Sarvas Melinda A Frye Adam J Chicco Simon J Lees Pro-inflammatory mediation of myoblast proliferation.
description	Skeletal muscle satellite cell function is largely dictated by the surrounding environment following injury. Immune cell infiltration dominates the extracellular space in the injured area, resulting in increased cytokine concentrations. While increased pro-inflammatory cytokine expression has been previously established in the first 3 days following injury, less is known about the time course of cytokine expression and the specific mechanisms of cytokine induced myoblast function. Therefore, the expression of IL-1β and IL-6 at several time points following injury, and their effects on myoblast proliferation, were examined. In order to do this, skeletal muscle was injured using barium chloride in mice and tissue was collected 1, 5, 10, and 28 days following injury. Mechanisms of cytokine induced proliferation were determined in cell culture using both primary and C2C12 myoblasts. It was found that there is a ∼20-fold increase in IL-1β (p≤0.05) and IL-6 (p = 0.06) expression 5 days following injury. IL-1β increased proliferation of both primary and C2C12 cells ∼25%. IL-1β stimulation also resulted in increased NF-κB activity, likely contributing to the increased proliferation. These data demonstrate for the first time that IL-1β alone can increase the mitogenic activity of primary skeletal muscle satellite cells and offer insight into the mechanisms dictating satellite cell function following injury.
format	article
author	Jeffrey S Otis Sarah Niccoli Nicole Hawdon Jessica L Sarvas Melinda A Frye Adam J Chicco Simon J Lees
author_facet	Jeffrey S Otis Sarah Niccoli Nicole Hawdon Jessica L Sarvas Melinda A Frye Adam J Chicco Simon J Lees
author_sort	Jeffrey S Otis
title	Pro-inflammatory mediation of myoblast proliferation.
title_short	Pro-inflammatory mediation of myoblast proliferation.
title_full	Pro-inflammatory mediation of myoblast proliferation.
title_fullStr	Pro-inflammatory mediation of myoblast proliferation.
title_full_unstemmed	Pro-inflammatory mediation of myoblast proliferation.
title_sort	pro-inflammatory mediation of myoblast proliferation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b0fca2be516c48079d5a5151485b47dc
work_keys_str_mv	AT jeffreysotis proinflammatorymediationofmyoblastproliferation AT sarahniccoli proinflammatorymediationofmyoblastproliferation AT nicolehawdon proinflammatorymediationofmyoblastproliferation AT jessicalsarvas proinflammatorymediationofmyoblastproliferation AT melindaafrye proinflammatorymediationofmyoblastproliferation AT adamjchicco proinflammatorymediationofmyoblastproliferation AT simonjlees proinflammatorymediationofmyoblastproliferation
_version_	1718421823741231104

Pro-inflammatory mediation of myoblast proliferation.

Ejemplares similares