Long noncoding RNAs promote transcriptional poising of inducible genes.

Long noncoding RNAs (lncRNAs) are a class of molecules that impinge on the expression of protein-coding genes. Previous studies have suggested that the GAL cluster-associated lncRNAs of Saccharomyces cerevisiae repress expression of the protein-coding GAL genes. Herein, we demonstrate a previously u...

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Autores principales:	Sara C Cloutier, Siwen Wang, Wai Kit Ma, Christopher J Petell, Elizabeth J Tran
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b104fe3844b44792909ad0b6cf59f605
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spelling	oai:doaj.org-article:b104fe3844b44792909ad0b6cf59f6052021-11-18T05:37:43ZLong noncoding RNAs promote transcriptional poising of inducible genes.1544-91731545-788510.1371/journal.pbio.1001715https://doaj.org/article/b104fe3844b44792909ad0b6cf59f6052013-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260025/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Long noncoding RNAs (lncRNAs) are a class of molecules that impinge on the expression of protein-coding genes. Previous studies have suggested that the GAL cluster-associated lncRNAs of Saccharomyces cerevisiae repress expression of the protein-coding GAL genes. Herein, we demonstrate a previously unrecognized role for the GAL lncRNAs in activating gene expression. In yeast strains lacking the RNA helicase, DBP2, or the RNA decay enzyme, XRN1, we find that the GAL lncRNAs specifically accelerate gene expression from a prior repressive state. Furthermore, we provide evidence that the previously suggested repressive role is a result of specific mutant phenotypes, rather than a reflection of the normal, wild-type function of these noncoding RNAs. To shed light on the mechanism for lncRNA-dependent gene activation, we show that rapid induction of the protein-coding GAL genes is associated with faster recruitment of RNA polymerase II and reduced association of transcriptional repressors with GAL gene promoters. This suggests that the GAL lncRNAs enhance expression by derepressing the GAL genes. Consistently, the GAL lncRNAs enhance the kinetics of transcriptional induction, promoting faster expression of the protein-coding GAL genes upon the switch in carbon source. We suggest that the GAL lncRNAs poise inducible genes for rapid activation, enabling cells to more effectively trigger new transcriptional programs in response to cellular cues.Sara C CloutierSiwen WangWai Kit MaChristopher J PetellElizabeth J TranPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 11, Iss 11, p e1001715 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Sara C Cloutier Siwen Wang Wai Kit Ma Christopher J Petell Elizabeth J Tran Long noncoding RNAs promote transcriptional poising of inducible genes.
description	Long noncoding RNAs (lncRNAs) are a class of molecules that impinge on the expression of protein-coding genes. Previous studies have suggested that the GAL cluster-associated lncRNAs of Saccharomyces cerevisiae repress expression of the protein-coding GAL genes. Herein, we demonstrate a previously unrecognized role for the GAL lncRNAs in activating gene expression. In yeast strains lacking the RNA helicase, DBP2, or the RNA decay enzyme, XRN1, we find that the GAL lncRNAs specifically accelerate gene expression from a prior repressive state. Furthermore, we provide evidence that the previously suggested repressive role is a result of specific mutant phenotypes, rather than a reflection of the normal, wild-type function of these noncoding RNAs. To shed light on the mechanism for lncRNA-dependent gene activation, we show that rapid induction of the protein-coding GAL genes is associated with faster recruitment of RNA polymerase II and reduced association of transcriptional repressors with GAL gene promoters. This suggests that the GAL lncRNAs enhance expression by derepressing the GAL genes. Consistently, the GAL lncRNAs enhance the kinetics of transcriptional induction, promoting faster expression of the protein-coding GAL genes upon the switch in carbon source. We suggest that the GAL lncRNAs poise inducible genes for rapid activation, enabling cells to more effectively trigger new transcriptional programs in response to cellular cues.
format	article
author	Sara C Cloutier Siwen Wang Wai Kit Ma Christopher J Petell Elizabeth J Tran
author_facet	Sara C Cloutier Siwen Wang Wai Kit Ma Christopher J Petell Elizabeth J Tran
author_sort	Sara C Cloutier
title	Long noncoding RNAs promote transcriptional poising of inducible genes.
title_short	Long noncoding RNAs promote transcriptional poising of inducible genes.
title_full	Long noncoding RNAs promote transcriptional poising of inducible genes.
title_fullStr	Long noncoding RNAs promote transcriptional poising of inducible genes.
title_full_unstemmed	Long noncoding RNAs promote transcriptional poising of inducible genes.
title_sort	long noncoding rnas promote transcriptional poising of inducible genes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b104fe3844b44792909ad0b6cf59f605
work_keys_str_mv	AT saraccloutier longnoncodingrnaspromotetranscriptionalpoisingofinduciblegenes AT siwenwang longnoncodingrnaspromotetranscriptionalpoisingofinduciblegenes AT waikitma longnoncodingrnaspromotetranscriptionalpoisingofinduciblegenes AT christopherjpetell longnoncodingrnaspromotetranscriptionalpoisingofinduciblegenes AT elizabethjtran longnoncodingrnaspromotetranscriptionalpoisingofinduciblegenes
_version_	1718424872672034816

Long noncoding RNAs promote transcriptional poising of inducible genes.

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