Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease

Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease

Chunxia Du,1,2,* Hua Xie,1,2,* Rujin Zang,1,2,* Ziyang Shen,1,2 Hongxing Li,1,2 Pingfa Chen,1,2 Xiaoqun Xu,1,2 Yankai Xia,2,3 Weibing Tang1,2 1Department of Pediatric Surgery, Nanjing Children’s Hospital Affiliated to Nanjing Medical University, 2State Key Laboratory of Reproductive Medic...

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Autores principales: Du C, Xie H, Zang R, Shen Z, Li H, Chen P, Xu X, Xia Y, Tang W
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
Hirschsprung disease
Neuronal development
Exosomal long non-coding RNA
Intercellular communication
Apoptosis
Mitochondria
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/b1061b101fe242cc9c07994358f73f38
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spelling oai:doaj.org-article:b1061b101fe242cc9c07994358f73f382021-12-02T04:21:05ZApoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease1178-2013https://doaj.org/article/b1061b101fe242cc9c07994358f73f382016-11-01T00:00:00Zhttps://www.dovepress.com/apoptotic-neuron-secreted-hn12-inhibits-cell-apoptosis-in-hirschsprung-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chunxia Du,1,2,* Hua Xie,1,2,* Rujin Zang,1,2,* Ziyang Shen,1,2 Hongxing Li,1,2 Pingfa Chen,1,2 Xiaoqun Xu,1,2 Yankai Xia,2,3 Weibing Tang1,2 1Department of Pediatric Surgery, Nanjing Children’s Hospital Affiliated to Nanjing Medical University, 2State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, 3Key Laboratory of Modern Toxicology, Ministry of Education, Nanjing Medical University, Nanjing, People’s Republic of China *These authors contributed equally to this work Abstract: Perturbation in apoptosis can lead to Hirschsprung’s disease (HSCR), which is a genetic disorder of neural crest development. It is believed that long noncoding RNAs (lncRNAs) play a role in the progression of HSCR. This study shows that apoptotic neurons can suppress apoptosis of nonapoptotic cells by secreting exosomes that contain high levels of HN12 lncRNA. Elevated exogenous HN12 in nonapoptotic cells effectively inhibited cell apoptosis by maintaining the function of mitochondria, including the production of ATP and the release of cytochrome C. These results demonstrate that secreted lncRNAs may serve as signaling molecules mediating intercellular communication in HSCR. In addition, high HN12 levels in the circulation worked as a biomarker for predicting HSCR, providing a potential, novel, noninvasive diagnostic approach for early screening of HSCR. Keywords: Hirschsprung’s disease, neuronal development, exosomal long noncoding RNA, intercellular communication, apoptosis, mitochondriaDu CXie HZang RShen ZLi HChen PXu XXia YTang WDove Medical PressarticleHirschsprung diseaseNeuronal developmentExosomal long non-coding RNAIntercellular communicationApoptosisMitochondriaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5871-5881 (2016)
institution DOAJ
collection DOAJ
language EN
topic Hirschsprung disease
Neuronal development
Exosomal long non-coding RNA
Intercellular communication
Apoptosis
Mitochondria
Medicine (General)
R5-920
spellingShingle Hirschsprung disease
Neuronal development
Exosomal long non-coding RNA
Intercellular communication
Apoptosis
Mitochondria
Medicine (General)
R5-920
Du C
Xie H
Zang R
Shen Z
Li H
Chen P
Xu X
Xia Y
Tang W
Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease
description Chunxia Du,1,2,* Hua Xie,1,2,* Rujin Zang,1,2,* Ziyang Shen,1,2 Hongxing Li,1,2 Pingfa Chen,1,2 Xiaoqun Xu,1,2 Yankai Xia,2,3 Weibing Tang1,2 1Department of Pediatric Surgery, Nanjing Children’s Hospital Affiliated to Nanjing Medical University, 2State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, 3Key Laboratory of Modern Toxicology, Ministry of Education, Nanjing Medical University, Nanjing, People’s Republic of China *These authors contributed equally to this work Abstract: Perturbation in apoptosis can lead to Hirschsprung’s disease (HSCR), which is a genetic disorder of neural crest development. It is believed that long noncoding RNAs (lncRNAs) play a role in the progression of HSCR. This study shows that apoptotic neurons can suppress apoptosis of nonapoptotic cells by secreting exosomes that contain high levels of HN12 lncRNA. Elevated exogenous HN12 in nonapoptotic cells effectively inhibited cell apoptosis by maintaining the function of mitochondria, including the production of ATP and the release of cytochrome C. These results demonstrate that secreted lncRNAs may serve as signaling molecules mediating intercellular communication in HSCR. In addition, high HN12 levels in the circulation worked as a biomarker for predicting HSCR, providing a potential, novel, noninvasive diagnostic approach for early screening of HSCR. Keywords: Hirschsprung’s disease, neuronal development, exosomal long noncoding RNA, intercellular communication, apoptosis, mitochondria
format article
author Du C
Xie H
Zang R
Shen Z
Li H
Chen P
Xu X
Xia Y
Tang W
author_facet Du C
Xie H
Zang R
Shen Z
Li H
Chen P
Xu X
Xia Y
Tang W
author_sort Du C
title Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease
title_short Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease
title_full Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease
title_fullStr Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease
title_full_unstemmed Apoptotic neuron-secreted HN12 inhibits cell apoptosis in Hirschsprung's disease
title_sort apoptotic neuron-secreted hn12 inhibits cell apoptosis in hirschsprung's disease
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/b1061b101fe242cc9c07994358f73f38
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AT xieh apoptoticneuronsecretedhn12inhibitscellapoptosisinhirschsprung39sdisease
AT zangr apoptoticneuronsecretedhn12inhibitscellapoptosisinhirschsprung39sdisease
AT shenz apoptoticneuronsecretedhn12inhibitscellapoptosisinhirschsprung39sdisease
AT lih apoptoticneuronsecretedhn12inhibitscellapoptosisinhirschsprung39sdisease
AT chenp apoptoticneuronsecretedhn12inhibitscellapoptosisinhirschsprung39sdisease
AT xux apoptoticneuronsecretedhn12inhibitscellapoptosisinhirschsprung39sdisease
AT xiay apoptoticneuronsecretedhn12inhibitscellapoptosisinhirschsprung39sdisease
AT tangw apoptoticneuronsecretedhn12inhibitscellapoptosisinhirschsprung39sdisease
_version_ 1718401328660611072

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