Massively parallel RNA device engineering in mammalian cells with RNA-Seq

Synthetic RNA-based devices can dynamically control a wide range of processes. Here the authors develop a quantitative and high-throughput mammalian cell-based RNA-seq assay to efficiently engineer ribozyme switches.

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Detalles Bibliográficos
Autores principales: Joy S. Xiang, Matias Kaplan, Peter Dykstra, Michaela Hinks, Maureen McKeague, Christina D. Smolke
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/b1085f5500c446be9254e89e2d3669c0
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Descripción
Sumario:Synthetic RNA-based devices can dynamically control a wide range of processes. Here the authors develop a quantitative and high-throughput mammalian cell-based RNA-seq assay to efficiently engineer ribozyme switches.