Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli

Abstract Antibiotic treatment kills a large portion of a population, while a small, tolerant subpopulation survives. Tolerant bacteria disrupt antibiotic efficacy and increase the likelihood that a population gains antibiotic resistance, a growing health concern. We examined how E. coli transcriptio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Heather S. Deter, Tahmina Hossain, Nicholas C. Butzin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b11a131108294e729838f3b24feafa52
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b11a131108294e729838f3b24feafa52
record_format	dspace
spelling	oai:doaj.org-article:b11a131108294e729838f3b24feafa522021-12-02T17:05:11ZAntibiotic tolerance is associated with a broad and complex transcriptional response in E. coli10.1038/s41598-021-85509-72045-2322https://doaj.org/article/b11a131108294e729838f3b24feafa522021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85509-7https://doaj.org/toc/2045-2322Abstract Antibiotic treatment kills a large portion of a population, while a small, tolerant subpopulation survives. Tolerant bacteria disrupt antibiotic efficacy and increase the likelihood that a population gains antibiotic resistance, a growing health concern. We examined how E. coli transcriptional networks changed in response to lethal ampicillin concentrations. We are the first to apply transcriptional regulatory network (TRN) analysis to antibiotic tolerance by leveraging existing knowledge and our transcriptional data. TRN analysis shows that gene expression changes specific to ampicillin treatment are likely caused by specific sigma and transcription factors typically regulated by proteolysis. These results demonstrate that to survive lethal concentration of ampicillin specific regulatory proteins change activity and cause a coordinated transcriptional response that leverages multiple gene systems.Heather S. DeterTahmina HossainNicholas C. ButzinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heather S. Deter Tahmina Hossain Nicholas C. Butzin Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli
description	Abstract Antibiotic treatment kills a large portion of a population, while a small, tolerant subpopulation survives. Tolerant bacteria disrupt antibiotic efficacy and increase the likelihood that a population gains antibiotic resistance, a growing health concern. We examined how E. coli transcriptional networks changed in response to lethal ampicillin concentrations. We are the first to apply transcriptional regulatory network (TRN) analysis to antibiotic tolerance by leveraging existing knowledge and our transcriptional data. TRN analysis shows that gene expression changes specific to ampicillin treatment are likely caused by specific sigma and transcription factors typically regulated by proteolysis. These results demonstrate that to survive lethal concentration of ampicillin specific regulatory proteins change activity and cause a coordinated transcriptional response that leverages multiple gene systems.
format	article
author	Heather S. Deter Tahmina Hossain Nicholas C. Butzin
author_facet	Heather S. Deter Tahmina Hossain Nicholas C. Butzin
author_sort	Heather S. Deter
title	Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli
title_short	Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli
title_full	Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli
title_fullStr	Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli
title_full_unstemmed	Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli
title_sort	antibiotic tolerance is associated with a broad and complex transcriptional response in e. coli
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b11a131108294e729838f3b24feafa52
work_keys_str_mv	AT heathersdeter antibiotictoleranceisassociatedwithabroadandcomplextranscriptionalresponseinecoli AT tahminahossain antibiotictoleranceisassociatedwithabroadandcomplextranscriptionalresponseinecoli AT nicholascbutzin antibiotictoleranceisassociatedwithabroadandcomplextranscriptionalresponseinecoli
_version_	1718381809535811584

Antibiotic tolerance is associated with a broad and complex transcriptional response in E. coli

Ejemplares similares