An animal model study on the gene expression profile of meniscal degeneration

Abstract Meniscal degeneration is a very common condition in elderly individuals, but the underlying mechanisms of its occurrence are not completely clear. This study examines the molecular mechanisms of meniscal degeneration. The anterior cruciate ligament (ACL) and lateral collateral ligament (LCL...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yehan Fang, Hui Huang, Gang Zhou, Qinghua Wang, Feng Gao, Chunbao Li, Yujie Liu, Jianping Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b11a34d8a2e74a6b96c0a57faec3687a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b11a34d8a2e74a6b96c0a57faec3687a
record_format	dspace
spelling	oai:doaj.org-article:b11a34d8a2e74a6b96c0a57faec3687a2021-12-02T15:11:51ZAn animal model study on the gene expression profile of meniscal degeneration10.1038/s41598-020-78349-42045-2322https://doaj.org/article/b11a34d8a2e74a6b96c0a57faec3687a2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78349-4https://doaj.org/toc/2045-2322Abstract Meniscal degeneration is a very common condition in elderly individuals, but the underlying mechanisms of its occurrence are not completely clear. This study examines the molecular mechanisms of meniscal degeneration. The anterior cruciate ligament (ACL) and lateral collateral ligament (LCL) of the right rear limbs of seven Wuzhishan mini-pigs were resected (meniscal degeneration group), and the left rear legs were sham-operated (control group). After 6 months, samples were taken for gene chip analysis, including differentially expressed gene (DEG) analysis, gene ontology (GO) analysis, clustering analysis, and pathway analysis. The selected 12 DEGs were validated by real time reverse transcription-polymerase chain reaction (RT-PCR). The two groups showed specific and highly clustered DEGs. A total of 893 DEGs were found, in which 537 are upregulated, and 356 are downregulated. The GO analysis showed that the significantly affected biological processes include nitric oxide metabolic process, male sex differentiation, and mesenchymal morphogenesis, the significantly affected cellular components include the endoplasmic reticulum membrane, and the significantly affected molecular functions include transition metal ion binding and iron ion binding. The pathway analysis showed that the significantly affected pathways include type II diabetes mellitus, inflammatory mediator regulation of TRP channels, and AMPK signaling pathway. The results of RT-PCR indicate that the microarray data accurately reflects the gene expression patterns. These findings indicate that several molecular mechanisms are involved in the development of meniscal degeneration, thus improving our understanding of meniscal degeneration and provide molecular therapeutic targets in the future.Yehan FangHui HuangGang ZhouQinghua WangFeng GaoChunbao LiYujie LiuJianping LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yehan Fang Hui Huang Gang Zhou Qinghua Wang Feng Gao Chunbao Li Yujie Liu Jianping Lin An animal model study on the gene expression profile of meniscal degeneration
description	Abstract Meniscal degeneration is a very common condition in elderly individuals, but the underlying mechanisms of its occurrence are not completely clear. This study examines the molecular mechanisms of meniscal degeneration. The anterior cruciate ligament (ACL) and lateral collateral ligament (LCL) of the right rear limbs of seven Wuzhishan mini-pigs were resected (meniscal degeneration group), and the left rear legs were sham-operated (control group). After 6 months, samples were taken for gene chip analysis, including differentially expressed gene (DEG) analysis, gene ontology (GO) analysis, clustering analysis, and pathway analysis. The selected 12 DEGs were validated by real time reverse transcription-polymerase chain reaction (RT-PCR). The two groups showed specific and highly clustered DEGs. A total of 893 DEGs were found, in which 537 are upregulated, and 356 are downregulated. The GO analysis showed that the significantly affected biological processes include nitric oxide metabolic process, male sex differentiation, and mesenchymal morphogenesis, the significantly affected cellular components include the endoplasmic reticulum membrane, and the significantly affected molecular functions include transition metal ion binding and iron ion binding. The pathway analysis showed that the significantly affected pathways include type II diabetes mellitus, inflammatory mediator regulation of TRP channels, and AMPK signaling pathway. The results of RT-PCR indicate that the microarray data accurately reflects the gene expression patterns. These findings indicate that several molecular mechanisms are involved in the development of meniscal degeneration, thus improving our understanding of meniscal degeneration and provide molecular therapeutic targets in the future.
format	article
author	Yehan Fang Hui Huang Gang Zhou Qinghua Wang Feng Gao Chunbao Li Yujie Liu Jianping Lin
author_facet	Yehan Fang Hui Huang Gang Zhou Qinghua Wang Feng Gao Chunbao Li Yujie Liu Jianping Lin
author_sort	Yehan Fang
title	An animal model study on the gene expression profile of meniscal degeneration
title_short	An animal model study on the gene expression profile of meniscal degeneration
title_full	An animal model study on the gene expression profile of meniscal degeneration
title_fullStr	An animal model study on the gene expression profile of meniscal degeneration
title_full_unstemmed	An animal model study on the gene expression profile of meniscal degeneration
title_sort	animal model study on the gene expression profile of meniscal degeneration
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b11a34d8a2e74a6b96c0a57faec3687a
work_keys_str_mv	AT yehanfang ananimalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT huihuang ananimalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT gangzhou ananimalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT qinghuawang ananimalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT fenggao ananimalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT chunbaoli ananimalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT yujieliu ananimalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT jianpinglin ananimalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT yehanfang animalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT huihuang animalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT gangzhou animalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT qinghuawang animalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT fenggao animalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT chunbaoli animalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT yujieliu animalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration AT jianpinglin animalmodelstudyonthegeneexpressionprofileofmeniscaldegeneration
_version_	1718387652632248320

An animal model study on the gene expression profile of meniscal degeneration

Ejemplares similares