An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis

Abstract Iron (Fe) is an essential element for plants; however, its availability is limited as it forms insoluble complexes in the soil. Consequently, plants have developed mechanisms to adapt to low Fe conditions. We demonstrate that ethylene is involved in Fe deficiency-induced physiological respo...

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Autores principales:	Wei Liu, Ting Wu, Qiwei Li, Xinzhong Zhang, Xuefeng Xu, Tianhong Li, Zhenhai Han, Yi Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b11b8e0c336f4a8990bc5b1df90a60e4
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spelling	oai:doaj.org-article:b11b8e0c336f4a8990bc5b1df90a60e42021-12-02T15:07:50ZAn ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis10.1038/s41598-018-19518-42045-2322https://doaj.org/article/b11b8e0c336f4a8990bc5b1df90a60e42018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19518-4https://doaj.org/toc/2045-2322Abstract Iron (Fe) is an essential element for plants; however, its availability is limited as it forms insoluble complexes in the soil. Consequently, plants have developed mechanisms to adapt to low Fe conditions. We demonstrate that ethylene is involved in Fe deficiency-induced physiological responses in Malus xiaojinensis, and describe the identification of MxERF4 as a protein-protein interaction partner with the MxFIT transcription factor, which is involved in the iron deficiency response. Furthermore, we demonstrate that MxERF4 acts as an MxFIT interaction partner to suppresses the expression of the Fe transporter MxIRT1, by binding directly to its promoter, requiring the EAR motif of the MxERF4 protein. Suppression of MxERF4 expression in M. xiaojinensis, using virus induced gene silencing resulted in an increase in MxIRT1 expression. Taken together, the results suggest a repression mechanism, where ethylene initiates the Fe deficiency response, and the response is then dampened, which may require a transient inhibition of Fe acquisition via the action of MxERF4.Wei LiuTing WuQiwei LiXinzhong ZhangXuefeng XuTianhong LiZhenhai HanYi WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Liu Ting Wu Qiwei Li Xinzhong Zhang Xuefeng Xu Tianhong Li Zhenhai Han Yi Wang An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis
description	Abstract Iron (Fe) is an essential element for plants; however, its availability is limited as it forms insoluble complexes in the soil. Consequently, plants have developed mechanisms to adapt to low Fe conditions. We demonstrate that ethylene is involved in Fe deficiency-induced physiological responses in Malus xiaojinensis, and describe the identification of MxERF4 as a protein-protein interaction partner with the MxFIT transcription factor, which is involved in the iron deficiency response. Furthermore, we demonstrate that MxERF4 acts as an MxFIT interaction partner to suppresses the expression of the Fe transporter MxIRT1, by binding directly to its promoter, requiring the EAR motif of the MxERF4 protein. Suppression of MxERF4 expression in M. xiaojinensis, using virus induced gene silencing resulted in an increase in MxIRT1 expression. Taken together, the results suggest a repression mechanism, where ethylene initiates the Fe deficiency response, and the response is then dampened, which may require a transient inhibition of Fe acquisition via the action of MxERF4.
format	article
author	Wei Liu Ting Wu Qiwei Li Xinzhong Zhang Xuefeng Xu Tianhong Li Zhenhai Han Yi Wang
author_facet	Wei Liu Ting Wu Qiwei Li Xinzhong Zhang Xuefeng Xu Tianhong Li Zhenhai Han Yi Wang
author_sort	Wei Liu
title	An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis
title_short	An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis
title_full	An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis
title_fullStr	An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis
title_full_unstemmed	An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis
title_sort	ethylene response factor (mxerf4) functions as a repressor of fe acquisition in malus xiaojinensis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b11b8e0c336f4a8990bc5b1df90a60e4
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An ethylene response factor (MxERF4) functions as a repressor of Fe acquisition in Malus xiaojinensis

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