Robotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis

Somjai Theanponkrang,1,2 Wipa Suginta,2,3 Helge Weingart,4 Mathias Winterhalter,4 Albert Schulte1,2 1School of Chemistry, 2Biochemistry–Electrochemistry Research Unit, Institute of Science, 3School of Biochemistry, Suranaree University of Technology, Nakhon Ratchasima, Thailand; 4Life Sci...

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Autores principales:	Theanponkrang S, Suginta W, Weingart H, Winterhalter M, Schulte A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b13435cba2ea49358947a203331c9ecd
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spelling	oai:doaj.org-article:b13435cba2ea49358947a203331c9ecd2021-12-02T03:05:06ZRobotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis1178-2013https://doaj.org/article/b13435cba2ea49358947a203331c9ecd2015-01-01T00:00:00Zhttp://www.dovepress.com/robotic-voltammetry-with-carbon-nanotube-based-sensors-a-superb-blend--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Somjai Theanponkrang,1,2 Wipa Suginta,2,3 Helge Weingart,4 Mathias Winterhalter,4 Albert Schulte1,2 1School of Chemistry, 2Biochemistry–Electrochemistry Research Unit, Institute of Science, 3School of Biochemistry, Suranaree University of Technology, Nakhon Ratchasima, Thailand; 4Life Sciences, School of Engineering and Science, Jacobs University Bremen, Bremen, Germany Abstract: A new automated pharmacoanalytical technique for convenient quantification of redox-active antibiotics has been established by combining the benefits of a carbon nanotube (CNT) sensor modification with electrocatalytic activity for analyte detection with the merits of a robotic electrochemical device that is capable of sequential nonmanual sample measurements in 24-well microtiter plates. Norfloxacin (NFX) and ciprofloxacin (CFX), two standard fluoroquinolone antibiotics, were used in automated calibration measurements by differential pulse voltammetry (DPV) and accomplished were linear ranges of 1–10 µM and 2–100 µM for NFX and CFX, respectively. The lowest detectable levels were estimated to be 0.3±0.1 µM (n=7) for NFX and 1.6±0.1 µM (n=7) for CFX. In standard solutions or tablet samples of known content, both analytes could be quantified with the robotic DPV microtiter plate assay, with recoveries within ±4% of 100%. And recoveries were as good when NFX was evaluated in human serum samples with added NFX. The use of simple instrumentation, convenience in execution, and high effectiveness in analyte quantitation suggest the merger between automated microtiter plate voltammetry and CNT-supported electrochemical drug detection as a novel methodology for antibiotic testing in pharmaceutical and clinical research and quality control laboratories. Keywords: antibiotics, electroanalysis, automation, microtiter plates, pharmaceutical screening, pharmacoanalyticsTheanponkrang SSuginta WWeingart HWinterhalter MSchulte ADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 859-868 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Theanponkrang S Suginta W Weingart H Winterhalter M Schulte A Robotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis
description	Somjai Theanponkrang,1,2 Wipa Suginta,2,3 Helge Weingart,4 Mathias Winterhalter,4 Albert Schulte1,2 1School of Chemistry, 2Biochemistry–Electrochemistry Research Unit, Institute of Science, 3School of Biochemistry, Suranaree University of Technology, Nakhon Ratchasima, Thailand; 4Life Sciences, School of Engineering and Science, Jacobs University Bremen, Bremen, Germany Abstract: A new automated pharmacoanalytical technique for convenient quantification of redox-active antibiotics has been established by combining the benefits of a carbon nanotube (CNT) sensor modification with electrocatalytic activity for analyte detection with the merits of a robotic electrochemical device that is capable of sequential nonmanual sample measurements in 24-well microtiter plates. Norfloxacin (NFX) and ciprofloxacin (CFX), two standard fluoroquinolone antibiotics, were used in automated calibration measurements by differential pulse voltammetry (DPV) and accomplished were linear ranges of 1–10 µM and 2–100 µM for NFX and CFX, respectively. The lowest detectable levels were estimated to be 0.3±0.1 µM (n=7) for NFX and 1.6±0.1 µM (n=7) for CFX. In standard solutions or tablet samples of known content, both analytes could be quantified with the robotic DPV microtiter plate assay, with recoveries within ±4% of 100%. And recoveries were as good when NFX was evaluated in human serum samples with added NFX. The use of simple instrumentation, convenience in execution, and high effectiveness in analyte quantitation suggest the merger between automated microtiter plate voltammetry and CNT-supported electrochemical drug detection as a novel methodology for antibiotic testing in pharmaceutical and clinical research and quality control laboratories. Keywords: antibiotics, electroanalysis, automation, microtiter plates, pharmaceutical screening, pharmacoanalytics
format	article
author	Theanponkrang S Suginta W Weingart H Winterhalter M Schulte A
author_facet	Theanponkrang S Suginta W Weingart H Winterhalter M Schulte A
author_sort	Theanponkrang S
title	Robotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis
title_short	Robotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis
title_full	Robotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis
title_fullStr	Robotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis
title_full_unstemmed	Robotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis
title_sort	robotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/b13435cba2ea49358947a203331c9ecd
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Robotic voltammetry with carbon nanotube-based sensors: a superb blend for convenient high-quality antimicrobial trace analysis

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