Therapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma

Abstract Gallbladder carcinoma (GBC) is the most common malignant tumour in the biliary tract, but effective therapeutics are lacking. Based on our previous studies, miR-135a is a potential tool to inhibit GBC proliferation. In this study, we constructed miR-135a-loaded DSPE-PEG2000 liposomes modifi...

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Autores principales:	Guanghua Yang, Baobing Yin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1343df2ae254a858681a78d1967827c
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spelling	oai:doaj.org-article:b1343df2ae254a858681a78d1967827c2021-12-02T16:06:32ZTherapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma10.1038/s41598-017-06234-82045-2322https://doaj.org/article/b1343df2ae254a858681a78d1967827c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06234-8https://doaj.org/toc/2045-2322Abstract Gallbladder carcinoma (GBC) is the most common malignant tumour in the biliary tract, but effective therapeutics are lacking. Based on our previous studies, miR-135a is a potential tool to inhibit GBC proliferation. In this study, we constructed miR-135a-loaded DSPE-PEG2000 liposomes modified with Anti-EGFR antibodies (Anti-EGFR-CIL-miR-135a). The results of an analysis of their physicochemical properties indicated the particle size of it was 222.0 ± 2.1 nm in diameter with an uptake efficiency of 86.5%. Next, the post-treatment biological behaviours of GBC, specifically, invasion, metastasis and apoptosis, were evaluated. miR-135a inhibited GBC invasion and metastasis and promoted apoptosis compared to controls. Additionally, miR-135a targeted and regulated the expression of ROCK1, HOXA10 and BCL-2. Due to the targeted effects of Anti-EGFR-CIL-miR-135a, the GBC tumour growth rate was 60% lower in an in vivo xenograft-bearing mouse model compared to controls. Thus, Anti-EGFR-CIL-miR-135a is a promising therapeutic strategy to combat GBC.Guanghua YangBaobing YinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guanghua Yang Baobing Yin Therapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma
description	Abstract Gallbladder carcinoma (GBC) is the most common malignant tumour in the biliary tract, but effective therapeutics are lacking. Based on our previous studies, miR-135a is a potential tool to inhibit GBC proliferation. In this study, we constructed miR-135a-loaded DSPE-PEG2000 liposomes modified with Anti-EGFR antibodies (Anti-EGFR-CIL-miR-135a). The results of an analysis of their physicochemical properties indicated the particle size of it was 222.0 ± 2.1 nm in diameter with an uptake efficiency of 86.5%. Next, the post-treatment biological behaviours of GBC, specifically, invasion, metastasis and apoptosis, were evaluated. miR-135a inhibited GBC invasion and metastasis and promoted apoptosis compared to controls. Additionally, miR-135a targeted and regulated the expression of ROCK1, HOXA10 and BCL-2. Due to the targeted effects of Anti-EGFR-CIL-miR-135a, the GBC tumour growth rate was 60% lower in an in vivo xenograft-bearing mouse model compared to controls. Thus, Anti-EGFR-CIL-miR-135a is a promising therapeutic strategy to combat GBC.
format	article
author	Guanghua Yang Baobing Yin
author_facet	Guanghua Yang Baobing Yin
author_sort	Guanghua Yang
title	Therapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma
title_short	Therapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma
title_full	Therapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma
title_fullStr	Therapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma
title_full_unstemmed	Therapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma
title_sort	therapeutic effects of long-circulating mir-135a-containing cationic immunoliposomes against gallbladder carcinoma
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b1343df2ae254a858681a78d1967827c
work_keys_str_mv	AT guanghuayang therapeuticeffectsoflongcirculatingmir135acontainingcationicimmunoliposomesagainstgallbladdercarcinoma AT baobingyin therapeuticeffectsoflongcirculatingmir135acontainingcationicimmunoliposomesagainstgallbladdercarcinoma
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Therapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma

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