Apparent Yield Stress of Sputum as a Relevant Biomarker in Cystic Fibrosis

The mucus obstructing the airways of Cystic Fibrosis (CF) patients is a yield stress fluid. Linear and non-linear rheological analyses of CF sputa can provide relevant biophysical markers, which could be used for the management of this disease. Sputa were collected from CF patients either without an...

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Autores principales:	Rosy Ghanem, Philippe Roquefort, Sophie Ramel, Véronique Laurent, Tanguy Haute, Tony Le Gall, Thierry Aubry, Tristan Montier
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	airway mucus rheology biomarker Cystic Fibrosis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b1351afc6bea459685cf5a3012e13a52
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spelling	oai:doaj.org-article:b1351afc6bea459685cf5a3012e13a522021-11-25T17:11:34ZApparent Yield Stress of Sputum as a Relevant Biomarker in Cystic Fibrosis10.3390/cells101131072073-4409https://doaj.org/article/b1351afc6bea459685cf5a3012e13a522021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3107https://doaj.org/toc/2073-4409The mucus obstructing the airways of Cystic Fibrosis (CF) patients is a yield stress fluid. Linear and non-linear rheological analyses of CF sputa can provide relevant biophysical markers, which could be used for the management of this disease. Sputa were collected from CF patients either without any induction or following an aerosol treatment with the recombinant human DNAse (rhDNAse, Pulmozyme<sup>®</sup>). Several sample preparations were considered and multiple measurements were performed in order to assess both the repeatability and the robustness of the rheological measurements. The linear and non-linear rheological properties of all CF sputa were characterized. While no correlation between oscillatory shear linear viscoelastic properties and clinical data was observed, the steady shear flow data showed that the apparent yield stress of sputum from CF patients previously treated with rhDNAse was approximately one decade lower than that of non-treated CF patients. Similar results were obtained with sputa from non-induced CF patients subjected ex vivo to a Pulmozyme<sup>®</sup> aerosol treatment. The results demonstrate that the apparent yield stress of patient sputa is a relevant predictive/prognostic biomarker in CF patients and could help in the development of new mucolytic agents.Rosy GhanemPhilippe RoquefortSophie RamelVéronique LaurentTanguy HauteTony Le GallThierry AubryTristan MontierMDPI AGarticleairway mucusrheologybiomarkerCystic FibrosisBiology (General)QH301-705.5ENCells, Vol 10, Iss 3107, p 3107 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	airway mucus rheology biomarker Cystic Fibrosis Biology (General) QH301-705.5
spellingShingle	airway mucus rheology biomarker Cystic Fibrosis Biology (General) QH301-705.5 Rosy Ghanem Philippe Roquefort Sophie Ramel Véronique Laurent Tanguy Haute Tony Le Gall Thierry Aubry Tristan Montier Apparent Yield Stress of Sputum as a Relevant Biomarker in Cystic Fibrosis
description	The mucus obstructing the airways of Cystic Fibrosis (CF) patients is a yield stress fluid. Linear and non-linear rheological analyses of CF sputa can provide relevant biophysical markers, which could be used for the management of this disease. Sputa were collected from CF patients either without any induction or following an aerosol treatment with the recombinant human DNAse (rhDNAse, Pulmozyme<sup>®</sup>). Several sample preparations were considered and multiple measurements were performed in order to assess both the repeatability and the robustness of the rheological measurements. The linear and non-linear rheological properties of all CF sputa were characterized. While no correlation between oscillatory shear linear viscoelastic properties and clinical data was observed, the steady shear flow data showed that the apparent yield stress of sputum from CF patients previously treated with rhDNAse was approximately one decade lower than that of non-treated CF patients. Similar results were obtained with sputa from non-induced CF patients subjected ex vivo to a Pulmozyme<sup>®</sup> aerosol treatment. The results demonstrate that the apparent yield stress of patient sputa is a relevant predictive/prognostic biomarker in CF patients and could help in the development of new mucolytic agents.
format	article
author	Rosy Ghanem Philippe Roquefort Sophie Ramel Véronique Laurent Tanguy Haute Tony Le Gall Thierry Aubry Tristan Montier
author_facet	Rosy Ghanem Philippe Roquefort Sophie Ramel Véronique Laurent Tanguy Haute Tony Le Gall Thierry Aubry Tristan Montier
author_sort	Rosy Ghanem
title	Apparent Yield Stress of Sputum as a Relevant Biomarker in Cystic Fibrosis
title_short	Apparent Yield Stress of Sputum as a Relevant Biomarker in Cystic Fibrosis
title_full	Apparent Yield Stress of Sputum as a Relevant Biomarker in Cystic Fibrosis
title_fullStr	Apparent Yield Stress of Sputum as a Relevant Biomarker in Cystic Fibrosis
title_full_unstemmed	Apparent Yield Stress of Sputum as a Relevant Biomarker in Cystic Fibrosis
title_sort	apparent yield stress of sputum as a relevant biomarker in cystic fibrosis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b1351afc6bea459685cf5a3012e13a52
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Apparent Yield Stress of Sputum as a Relevant Biomarker in Cystic Fibrosis

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