Anti-Nociceptive and Anti-Inflammatory Activity of Hygrophila schulli Leaves

Gebretsadkan Hintsa Tekulu,1 Abrha Desta,1 Mebrahtom Gebrelibanos Hiben,1 Ephrem Mebrahtu Araya2 1Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Ethiopia; 2Department of Pharmacy, College of Medicine and Health Sciences, Adigrat University,...

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Autores principales:	Tekulu GH, Desta A, Hiben MG, Araya EM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	pain inflammation carrageenan hyaluronidase hygrophila schulli Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/b13569b82343430491e6d0f887345bfc
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spelling	oai:doaj.org-article:b13569b82343430491e6d0f887345bfc2021-12-02T09:13:16ZAnti-Nociceptive and Anti-Inflammatory Activity of Hygrophila schulli Leaves1178-7031https://doaj.org/article/b13569b82343430491e6d0f887345bfc2020-09-01T00:00:00Zhttps://www.dovepress.com/anti-nociceptive-and-anti-inflammatory-activity-of-hygrophila-schulli--peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Gebretsadkan Hintsa Tekulu,1 Abrha Desta,1 Mebrahtom Gebrelibanos Hiben,1 Ephrem Mebrahtu Araya2 1Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Ethiopia; 2Department of Pharmacy, College of Medicine and Health Sciences, Adigrat University, Adigrat, EthiopiaCorrespondence: Gebretsadkan Hintsa Tekulu Tel +251 914277916Email gere.maysuru@gmail.comPurpose: The management of pain and inflammation with non-steroidal anti-inflammatory drugs and opioid analgesics are currently encountering severe adverse reactions. To overcome these problems, herbal remedies may offer new alternative medicines. Hygrophila schulli is a medicinal plant traditionally used for the treatment of pain and inflammation-related disorders; yet, these claims are not scientifically validated. Hence, this study was aimed to validate the traditional use of Hygrophila schulli leaves as anti-inflammatory and analgesic remedy.Methods: In vitro anti-hyaluronidase assay and in vivo carrageenan-induced hind paw oedema model were used to evaluate the anti-inflammatory property of ethanolic leaf extract of Hygrophila schulli. Tail immersion and acetic acid-induced writhing tests were performed to determine the central and peripheral analgesic activity of the leaf extract, respectively.Results: The ethanolic leaf extract exhibited significant anti-hyaluronidase activity (P< 0.001) and significant inhibition of carrageenan-induced paw oedema (P< 0.05) compared to untreated controls. Similarly, the extract significantly prolonged the reaction time of mice (P< 0.05) for the hot-water stimuli. Furthermore, an oral dose of the extract showed significant inhibition (P< 0.01) of acetic acid-induced abdominal contractions of mice. Besides, the ethanolic leaf extract did not cause any obvious sign of acute toxicity at a single oral dose of 2 g/kg.Conclusion: The findings of this study may partially support the acclaimed traditional use of Hygrophila schulli leaves for the treatment of pain and inflammatory conditions.Keywords: pain, inflammation, carrageenan, hyaluronidase, Hygrophila schulliTekulu GHDesta AHiben MGAraya EMDove Medical Pressarticlepaininflammationcarrageenanhyaluronidasehygrophila schulliPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 497-505 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	pain inflammation carrageenan hyaluronidase hygrophila schulli Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	pain inflammation carrageenan hyaluronidase hygrophila schulli Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Tekulu GH Desta A Hiben MG Araya EM Anti-Nociceptive and Anti-Inflammatory Activity of Hygrophila schulli Leaves
description	Gebretsadkan Hintsa Tekulu,1 Abrha Desta,1 Mebrahtom Gebrelibanos Hiben,1 Ephrem Mebrahtu Araya2 1Department of Pharmacognosy, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Ethiopia; 2Department of Pharmacy, College of Medicine and Health Sciences, Adigrat University, Adigrat, EthiopiaCorrespondence: Gebretsadkan Hintsa Tekulu Tel +251 914277916Email gere.maysuru@gmail.comPurpose: The management of pain and inflammation with non-steroidal anti-inflammatory drugs and opioid analgesics are currently encountering severe adverse reactions. To overcome these problems, herbal remedies may offer new alternative medicines. Hygrophila schulli is a medicinal plant traditionally used for the treatment of pain and inflammation-related disorders; yet, these claims are not scientifically validated. Hence, this study was aimed to validate the traditional use of Hygrophila schulli leaves as anti-inflammatory and analgesic remedy.Methods: In vitro anti-hyaluronidase assay and in vivo carrageenan-induced hind paw oedema model were used to evaluate the anti-inflammatory property of ethanolic leaf extract of Hygrophila schulli. Tail immersion and acetic acid-induced writhing tests were performed to determine the central and peripheral analgesic activity of the leaf extract, respectively.Results: The ethanolic leaf extract exhibited significant anti-hyaluronidase activity (P< 0.001) and significant inhibition of carrageenan-induced paw oedema (P< 0.05) compared to untreated controls. Similarly, the extract significantly prolonged the reaction time of mice (P< 0.05) for the hot-water stimuli. Furthermore, an oral dose of the extract showed significant inhibition (P< 0.01) of acetic acid-induced abdominal contractions of mice. Besides, the ethanolic leaf extract did not cause any obvious sign of acute toxicity at a single oral dose of 2 g/kg.Conclusion: The findings of this study may partially support the acclaimed traditional use of Hygrophila schulli leaves for the treatment of pain and inflammatory conditions.Keywords: pain, inflammation, carrageenan, hyaluronidase, Hygrophila schulli
format	article
author	Tekulu GH Desta A Hiben MG Araya EM
author_facet	Tekulu GH Desta A Hiben MG Araya EM
author_sort	Tekulu GH
title	Anti-Nociceptive and Anti-Inflammatory Activity of Hygrophila schulli Leaves
title_short	Anti-Nociceptive and Anti-Inflammatory Activity of Hygrophila schulli Leaves
title_full	Anti-Nociceptive and Anti-Inflammatory Activity of Hygrophila schulli Leaves
title_fullStr	Anti-Nociceptive and Anti-Inflammatory Activity of Hygrophila schulli Leaves
title_full_unstemmed	Anti-Nociceptive and Anti-Inflammatory Activity of Hygrophila schulli Leaves
title_sort	anti-nociceptive and anti-inflammatory activity of hygrophila schulli leaves
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/b13569b82343430491e6d0f887345bfc
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Anti-Nociceptive and Anti-Inflammatory Activity of Hygrophila schulli Leaves

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