Phosphorus Starvation- and Zinc Excess-Induced <i>Astragalus sinicus</i> AsZIP2 Zinc Transporter Is Suppressed by Arbuscular Mycorrhizal Symbiosis

Zinc (Zn) is one of the most essential micronutrients for plant growth and metabolism, but Zn excess can impair many basic metabolic processes in plant cells. In agriculture, crops often experience low phosphate (Pi) and high Zn double nutrient stresses because of inordinate agro-industrial activiti...

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Autores principales:	Xianan Xie, Xiaoning Fan, Hui Chen, Ming Tang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Zinc phosphate arbuscular mycorrhizal fungi <i>Astragalus sinicus</i> <i>ZIP</i> gene family AsZIP2 transporter Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b142aed724db4779a7c41f354b970355
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spelling	oai:doaj.org-article:b142aed724db4779a7c41f354b9703552021-11-25T18:05:30ZPhosphorus Starvation- and Zinc Excess-Induced <i>Astragalus sinicus</i> AsZIP2 Zinc Transporter Is Suppressed by Arbuscular Mycorrhizal Symbiosis10.3390/jof71108922309-608Xhttps://doaj.org/article/b142aed724db4779a7c41f354b9703552021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/892https://doaj.org/toc/2309-608XZinc (Zn) is one of the most essential micronutrients for plant growth and metabolism, but Zn excess can impair many basic metabolic processes in plant cells. In agriculture, crops often experience low phosphate (Pi) and high Zn double nutrient stresses because of inordinate agro-industrial activities, while the dual benefit of arbuscular mycorrhizal (AM) fungi protects plants from experiencing both deficient and toxic nutrient stresses. Although crosstalk between Pi and Zn nutrients in plants have been extensively studied at the physiological level, the molecular basis of how Pi starvation triggers Zn over-accumulation in plants and how AM plants coordinately modulate the Pi and Zn nutrient homeostasis remains to be elucidated. Here, we report that a novel <i>AsZIP2</i> gene, a Chinese milk vetch (<i>Astragalus sinicus</i>) member of the <i>ZIP</i> gene family, participates in the interaction between Pi and Zn nutrient homeostasis in plants. Phylogenetic analysis revealed that this AsZIP2 protein was closely related to the orthologous Medicago MtZIP2 and Arabidopsis AtZIP2 transporters. Gene expression analysis indicated that <i>AsZIP2</i> was highly induced in roots by Pi starvation or Zn excess yet attenuated by arbuscular mycorrhization in a Pi-dependent manner. Subcellular localization and heterologous expression experiments further showed that <i>AsZIP2</i> encoded a functional plasma membrane-localized transporter that mediated Zn uptake in yeast. Moreover, overexpression of <i>AsZIP2</i> in <i>A. sinicus</i> resulted in the over-accumulation of Zn concentration in roots at low Pi or excessive Zn concentrations, whereas <i>AsZIP2</i> silencing lines displayed an even more reduced Zn concentration than control lines under such conditions. Our results reveal that the AsZIP2 transporter functioned in Zn over-accumulation in roots during Pi starvation or high Zn supply but was repressed by AM symbiosis in a Pi-dependent manner. These findings also provide new insights into the <i>AsZIP2</i> gene acting in the regulation of Zn homeostasis in mycorrhizal plants through Pi signal.Xianan XieXiaoning FanHui ChenMing TangMDPI AGarticleZincphosphatearbuscular mycorrhizal fungi<i>Astragalus sinicus</i><i>ZIP</i> gene familyAsZIP2 transporterBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 892, p 892 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zinc phosphate arbuscular mycorrhizal fungi <i>Astragalus sinicus</i> <i>ZIP</i> gene family AsZIP2 transporter Biology (General) QH301-705.5
spellingShingle	Zinc phosphate arbuscular mycorrhizal fungi <i>Astragalus sinicus</i> <i>ZIP</i> gene family AsZIP2 transporter Biology (General) QH301-705.5 Xianan Xie Xiaoning Fan Hui Chen Ming Tang Phosphorus Starvation- and Zinc Excess-Induced <i>Astragalus sinicus</i> AsZIP2 Zinc Transporter Is Suppressed by Arbuscular Mycorrhizal Symbiosis
description	Zinc (Zn) is one of the most essential micronutrients for plant growth and metabolism, but Zn excess can impair many basic metabolic processes in plant cells. In agriculture, crops often experience low phosphate (Pi) and high Zn double nutrient stresses because of inordinate agro-industrial activities, while the dual benefit of arbuscular mycorrhizal (AM) fungi protects plants from experiencing both deficient and toxic nutrient stresses. Although crosstalk between Pi and Zn nutrients in plants have been extensively studied at the physiological level, the molecular basis of how Pi starvation triggers Zn over-accumulation in plants and how AM plants coordinately modulate the Pi and Zn nutrient homeostasis remains to be elucidated. Here, we report that a novel <i>AsZIP2</i> gene, a Chinese milk vetch (<i>Astragalus sinicus</i>) member of the <i>ZIP</i> gene family, participates in the interaction between Pi and Zn nutrient homeostasis in plants. Phylogenetic analysis revealed that this AsZIP2 protein was closely related to the orthologous Medicago MtZIP2 and Arabidopsis AtZIP2 transporters. Gene expression analysis indicated that <i>AsZIP2</i> was highly induced in roots by Pi starvation or Zn excess yet attenuated by arbuscular mycorrhization in a Pi-dependent manner. Subcellular localization and heterologous expression experiments further showed that <i>AsZIP2</i> encoded a functional plasma membrane-localized transporter that mediated Zn uptake in yeast. Moreover, overexpression of <i>AsZIP2</i> in <i>A. sinicus</i> resulted in the over-accumulation of Zn concentration in roots at low Pi or excessive Zn concentrations, whereas <i>AsZIP2</i> silencing lines displayed an even more reduced Zn concentration than control lines under such conditions. Our results reveal that the AsZIP2 transporter functioned in Zn over-accumulation in roots during Pi starvation or high Zn supply but was repressed by AM symbiosis in a Pi-dependent manner. These findings also provide new insights into the <i>AsZIP2</i> gene acting in the regulation of Zn homeostasis in mycorrhizal plants through Pi signal.
format	article
author	Xianan Xie Xiaoning Fan Hui Chen Ming Tang
author_facet	Xianan Xie Xiaoning Fan Hui Chen Ming Tang
author_sort	Xianan Xie
title	Phosphorus Starvation- and Zinc Excess-Induced <i>Astragalus sinicus</i> AsZIP2 Zinc Transporter Is Suppressed by Arbuscular Mycorrhizal Symbiosis
title_short	Phosphorus Starvation- and Zinc Excess-Induced <i>Astragalus sinicus</i> AsZIP2 Zinc Transporter Is Suppressed by Arbuscular Mycorrhizal Symbiosis
title_full	Phosphorus Starvation- and Zinc Excess-Induced <i>Astragalus sinicus</i> AsZIP2 Zinc Transporter Is Suppressed by Arbuscular Mycorrhizal Symbiosis
title_fullStr	Phosphorus Starvation- and Zinc Excess-Induced <i>Astragalus sinicus</i> AsZIP2 Zinc Transporter Is Suppressed by Arbuscular Mycorrhizal Symbiosis
title_full_unstemmed	Phosphorus Starvation- and Zinc Excess-Induced <i>Astragalus sinicus</i> AsZIP2 Zinc Transporter Is Suppressed by Arbuscular Mycorrhizal Symbiosis
title_sort	phosphorus starvation- and zinc excess-induced <i>astragalus sinicus</i> aszip2 zinc transporter is suppressed by arbuscular mycorrhizal symbiosis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b142aed724db4779a7c41f354b970355
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Phosphorus Starvation- and Zinc Excess-Induced <i>Astragalus sinicus</i> AsZIP2 Zinc Transporter Is Suppressed by Arbuscular Mycorrhizal Symbiosis

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