Epigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients

Background:FOXP3 gene, known to be a potential tumor suppressor, has been identified to interact with HER2 in mammary cancer. Moreover, the high expression of FOXP3 serves as a good predictor of the survival of patients in breast cancer, prostate cancer, and gastric cancer. The expression and epigen...

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Autores principales:	Sadaf, Naseem Akhter, Raed A. Alharbi, Abdulmajid A. A. Sindi, Mohammad Zeeshan Najm, Fahad A. Alhumaydhi, Mohammad Aasif Khan, S.V.S Deo, Syed Akhtar Husain
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	PCR-real time methylation specific PCR (MS PCR) immunohistochemistry (IHC) biomarkers mammary cancer gene experession Genetics QH426-470
Acceso en línea:	https://doaj.org/article/b144c0f59b30457782051db2491fbf14
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spelling	oai:doaj.org-article:b144c0f59b30457782051db2491fbf142021-12-01T13:27:51ZEpigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients1664-802110.3389/fgene.2021.781400https://doaj.org/article/b144c0f59b30457782051db2491fbf142021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.781400/fullhttps://doaj.org/toc/1664-8021Background:FOXP3 gene, known to be a potential tumor suppressor, has been identified to interact with HER2 in mammary cancer. Moreover, the high expression of FOXP3 serves as a good predictor of the survival of patients in breast cancer, prostate cancer, and gastric cancer. The expression and epigenetic alterations were evaluated in female breast cancer patients.Material and Methods: Expression studies at the mRNA level and protein level were conducted in 140 breast cancer cases by real-time PCR and immunohistochemistry, respectively. Epigenetic studies were also conducted by analyzing the methylation status at the promoter region of the gene using MS-PCR.Results:FOXP3 mRNA expression and protein expression were downregulated in breast cancer patients. The absence of FOXP3 protein expression is significantly associated with promoter methylation, where 70 methylated cases exhibited protein loss (70/95, 73.6%). Statistically, we also found a significant correlation between FOXP3 protein expression and TNM stage, promoter methylation, and histological grade. The methylated FOXP3 cases that did not express protein were also significantly associated with positive lymph node metastasis and HER-2 status.Conclusion: The expression profile of FOXP3 may serve as a prognostic factor. In short, FOXP3 may stand in the most crucial list of biomarkers for breast cancer, bringing compelling results in terms of treatment and management of the disease. SadafNaseem AkhterRaed A. AlharbiAbdulmajid A. A. SindiMohammad Zeeshan NajmFahad A. AlhumaydhiMohammad Aasif KhanS.V.S DeoSyed Akhtar HusainFrontiers Media S.A.articlePCR-real timemethylation specific PCR (MS PCR)immunohistochemistry (IHC)biomarkersmammary cancergene experessionGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PCR-real time methylation specific PCR (MS PCR) immunohistochemistry (IHC) biomarkers mammary cancer gene experession Genetics QH426-470
spellingShingle	PCR-real time methylation specific PCR (MS PCR) immunohistochemistry (IHC) biomarkers mammary cancer gene experession Genetics QH426-470 Sadaf Naseem Akhter Raed A. Alharbi Abdulmajid A. A. Sindi Mohammad Zeeshan Najm Fahad A. Alhumaydhi Mohammad Aasif Khan S.V.S Deo Syed Akhtar Husain Epigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients
description	Background:FOXP3 gene, known to be a potential tumor suppressor, has been identified to interact with HER2 in mammary cancer. Moreover, the high expression of FOXP3 serves as a good predictor of the survival of patients in breast cancer, prostate cancer, and gastric cancer. The expression and epigenetic alterations were evaluated in female breast cancer patients.Material and Methods: Expression studies at the mRNA level and protein level were conducted in 140 breast cancer cases by real-time PCR and immunohistochemistry, respectively. Epigenetic studies were also conducted by analyzing the methylation status at the promoter region of the gene using MS-PCR.Results:FOXP3 mRNA expression and protein expression were downregulated in breast cancer patients. The absence of FOXP3 protein expression is significantly associated with promoter methylation, where 70 methylated cases exhibited protein loss (70/95, 73.6%). Statistically, we also found a significant correlation between FOXP3 protein expression and TNM stage, promoter methylation, and histological grade. The methylated FOXP3 cases that did not express protein were also significantly associated with positive lymph node metastasis and HER-2 status.Conclusion: The expression profile of FOXP3 may serve as a prognostic factor. In short, FOXP3 may stand in the most crucial list of biomarkers for breast cancer, bringing compelling results in terms of treatment and management of the disease.
format	article
author	Sadaf Naseem Akhter Raed A. Alharbi Abdulmajid A. A. Sindi Mohammad Zeeshan Najm Fahad A. Alhumaydhi Mohammad Aasif Khan S.V.S Deo Syed Akhtar Husain
author_facet	Sadaf Naseem Akhter Raed A. Alharbi Abdulmajid A. A. Sindi Mohammad Zeeshan Najm Fahad A. Alhumaydhi Mohammad Aasif Khan S.V.S Deo Syed Akhtar Husain
author_sort	Sadaf
title	Epigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients
title_short	Epigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients
title_full	Epigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients
title_fullStr	Epigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients
title_full_unstemmed	Epigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients
title_sort	epigenetic alteration and its association with downregulated foxp3 gene in indian breast cancer patients
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b144c0f59b30457782051db2491fbf14
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Epigenetic Alteration and its Association With Downregulated FOXP3 Gene in Indian Breast Cancer Patients

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