Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.

Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since s...

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Autores principales:	Ping Xu, Irina Mohorianu, Li Yang, Hansheng Zhao, Zhimin Gao, Tamas Dalmay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b14628a1a1e84554a10e65da727d6635
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spelling	oai:doaj.org-article:b14628a1a1e84554a10e65da727d66352021-11-25T06:06:29ZSmall RNA profile in moso bamboo root and leaf obtained by high definition adapters.1932-620310.1371/journal.pone.0103590https://doaj.org/article/b14628a1a1e84554a10e65da727d66352014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25079776/?tool=EBIhttps://doaj.org/toc/1932-6203Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs and a few other small non-coding RNAs that were not microRNAs. The biological implication of microRNA expression levels and targets of microRNAs are discussed.Ping XuIrina MohorianuLi YangHansheng ZhaoZhimin GaoTamas DalmayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e103590 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ping Xu Irina Mohorianu Li Yang Hansheng Zhao Zhimin Gao Tamas Dalmay Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.
description	Moso bamboo (Phyllostachy heterocycla cv. pubescens L.) is an economically important fast-growing tree. In order to gain better understanding of gene expression regulation in this important species we used next generation sequencing to profile small RNAs in leaf and roots of young seedlings. Since standard kits to produce cDNA of small RNAs are biased for certain small RNAs, we used High Definition adapters that reduce ligation bias. We identified and experimentally validated five new microRNAs and a few other small non-coding RNAs that were not microRNAs. The biological implication of microRNA expression levels and targets of microRNAs are discussed.
format	article
author	Ping Xu Irina Mohorianu Li Yang Hansheng Zhao Zhimin Gao Tamas Dalmay
author_facet	Ping Xu Irina Mohorianu Li Yang Hansheng Zhao Zhimin Gao Tamas Dalmay
author_sort	Ping Xu
title	Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.
title_short	Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.
title_full	Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.
title_fullStr	Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.
title_full_unstemmed	Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.
title_sort	small rna profile in moso bamboo root and leaf obtained by high definition adapters.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b14628a1a1e84554a10e65da727d6635
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Small RNA profile in moso bamboo root and leaf obtained by high definition adapters.

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