Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification

Ubiquitylation and SUMOylation are two important related post-translational modifications. Here the authors present an approach for the simultaneous identification and quantification of protein-wide SUMO and ubiquitin sites from a single sample, uncovering widespread crosstalk between the two modifi...

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Autores principales: Frédéric Lamoliatte, Francis P. McManus, Ghizlane Maarifi, Mounira K. Chelbi-Alix, Pierre Thibault
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/b154cff5cc4243dfbe1fb2ed128260a0
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spelling oai:doaj.org-article:b154cff5cc4243dfbe1fb2ed128260a02021-12-02T15:38:51ZUncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification10.1038/ncomms141092041-1723https://doaj.org/article/b154cff5cc4243dfbe1fb2ed128260a02017-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms14109https://doaj.org/toc/2041-1723Ubiquitylation and SUMOylation are two important related post-translational modifications. Here the authors present an approach for the simultaneous identification and quantification of protein-wide SUMO and ubiquitin sites from a single sample, uncovering widespread crosstalk between the two modifications.Frédéric LamoliatteFrancis P. McManusGhizlane MaarifiMounira K. Chelbi-AlixPierre ThibaultNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Frédéric Lamoliatte
Francis P. McManus
Ghizlane Maarifi
Mounira K. Chelbi-Alix
Pierre Thibault
Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification
description Ubiquitylation and SUMOylation are two important related post-translational modifications. Here the authors present an approach for the simultaneous identification and quantification of protein-wide SUMO and ubiquitin sites from a single sample, uncovering widespread crosstalk between the two modifications.
format article
author Frédéric Lamoliatte
Francis P. McManus
Ghizlane Maarifi
Mounira K. Chelbi-Alix
Pierre Thibault
author_facet Frédéric Lamoliatte
Francis P. McManus
Ghizlane Maarifi
Mounira K. Chelbi-Alix
Pierre Thibault
author_sort Frédéric Lamoliatte
title Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification
title_short Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification
title_full Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification
title_fullStr Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification
title_full_unstemmed Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification
title_sort uncovering the sumoylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/b154cff5cc4243dfbe1fb2ed128260a0
work_keys_str_mv AT fredericlamoliatte uncoveringthesumoylationandubiquitylationcrosstalkinhumancellsusingsequentialpeptideimmunopurification
AT francispmcmanus uncoveringthesumoylationandubiquitylationcrosstalkinhumancellsusingsequentialpeptideimmunopurification
AT ghizlanemaarifi uncoveringthesumoylationandubiquitylationcrosstalkinhumancellsusingsequentialpeptideimmunopurification
AT mounirakchelbialix uncoveringthesumoylationandubiquitylationcrosstalkinhumancellsusingsequentialpeptideimmunopurification
AT pierrethibault uncoveringthesumoylationandubiquitylationcrosstalkinhumancellsusingsequentialpeptideimmunopurification
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