Efficient E. coli expression strategies for production of soluble human crystallin ALDH3A1.
Aldehyde dehydrogenase 3A1 (ALDH3A1) is a recently characterized corneal crystallin with its exact functions still being unclear. Expressing recombinant human ALDH3A1 has been difficult in Escherichia coli (E. coli) because of low solubility, yield and insufficient purity issues. In this report, we...
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Autores principales: | Georgia-Persephoni Voulgaridou, Theodora Mantso, Katerina Chlichlia, Mihalis I Panayiotidis, Aglaia Pappa |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2013
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Materias: | |
Acceso en línea: | https://doaj.org/article/b15b5ecac5d04c2799a1b8302e5af4af |
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