Integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma

Abstract Background Traditional Chinese medicine (TCM) has a long history of treating glaucoma with remarkable effects, but there is no clear conclusion on its mechanism. Methods Network pharmacology and molecular docking were used to analyze the mechanism and targets of TCM in the treatment of glau...

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Autores principales:	Jiawei Yang, Mingxu Zhang, Qiuyi Song, Siqi Li, Xiulan Zhao, Liping Kan, Siquan Zhu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Glaucoma Baicalein Network pharmacological Experimental validation Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/b15b702b461147b1af5a9357944be66f
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spelling	oai:doaj.org-article:b15b702b461147b1af5a9357944be66f2021-11-28T12:08:20ZIntegrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma10.1186/s13020-021-00537-91749-8546https://doaj.org/article/b15b702b461147b1af5a9357944be66f2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13020-021-00537-9https://doaj.org/toc/1749-8546Abstract Background Traditional Chinese medicine (TCM) has a long history of treating glaucoma with remarkable effects, but there is no clear conclusion on its mechanism. Methods Network pharmacology and molecular docking were used to analyze the mechanism and targets of TCM in the treatment of glaucoma, and baicalein was used to treat chronic ocular hypertension animal models rats for observation. Results The results of animal experiments showed that baicalein could significantly reduce intraocular pressure (IOP) in a rat model of chronic ocular hypertension and protect the structure of the retina and optic nerve, as shown by hematoxylin–eosin (H&E) staining and transmission electron microscopy (TEM). Reducing the apoptosis of retinal ganglion cells (RGCs) by upregulating the expression of the antiapoptotic protein BCL-2 is basically consistent with the results of molecular docking. In the network pharmacology analysis, many key proteins of biological pathways involved in the herbal therapeutic processes in glaucoma, such as threonine kinase 1 (AKT1, core protein of PI3K/AKT signaling), tumor protein p53 (TP53, a tumor suppressor gene coding tumor protein P53), signal transducer and activator of transcription 3 (STAT3, core protein of JAK/STAT signaling), interleukin 6 (IL-6) and interleukin 17 (IL-17, proinflammatory factors), were identified. Their interactions built complicated chain reactions in the process of glaucoma. Conclusion By combining the analysis of network pharmacology and animal experimental results, baicalein could effectively improve the symptoms of glaucoma and reduce RGC apoptosis, suggesting that the potential mechanism of TCM in treating glaucoma is related to regulating inflammation and cellular immunity and reducing apoptosis.Jiawei YangMingxu ZhangQiuyi SongSiqi LiXiulan ZhaoLiping KanSiquan ZhuBMCarticleGlaucomaBaicaleinNetwork pharmacologicalExperimental validationOther systems of medicineRZ201-999ENChinese Medicine, Vol 16, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Glaucoma Baicalein Network pharmacological Experimental validation Other systems of medicine RZ201-999
spellingShingle	Glaucoma Baicalein Network pharmacological Experimental validation Other systems of medicine RZ201-999 Jiawei Yang Mingxu Zhang Qiuyi Song Siqi Li Xiulan Zhao Liping Kan Siquan Zhu Integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma
description	Abstract Background Traditional Chinese medicine (TCM) has a long history of treating glaucoma with remarkable effects, but there is no clear conclusion on its mechanism. Methods Network pharmacology and molecular docking were used to analyze the mechanism and targets of TCM in the treatment of glaucoma, and baicalein was used to treat chronic ocular hypertension animal models rats for observation. Results The results of animal experiments showed that baicalein could significantly reduce intraocular pressure (IOP) in a rat model of chronic ocular hypertension and protect the structure of the retina and optic nerve, as shown by hematoxylin–eosin (H&E) staining and transmission electron microscopy (TEM). Reducing the apoptosis of retinal ganglion cells (RGCs) by upregulating the expression of the antiapoptotic protein BCL-2 is basically consistent with the results of molecular docking. In the network pharmacology analysis, many key proteins of biological pathways involved in the herbal therapeutic processes in glaucoma, such as threonine kinase 1 (AKT1, core protein of PI3K/AKT signaling), tumor protein p53 (TP53, a tumor suppressor gene coding tumor protein P53), signal transducer and activator of transcription 3 (STAT3, core protein of JAK/STAT signaling), interleukin 6 (IL-6) and interleukin 17 (IL-17, proinflammatory factors), were identified. Their interactions built complicated chain reactions in the process of glaucoma. Conclusion By combining the analysis of network pharmacology and animal experimental results, baicalein could effectively improve the symptoms of glaucoma and reduce RGC apoptosis, suggesting that the potential mechanism of TCM in treating glaucoma is related to regulating inflammation and cellular immunity and reducing apoptosis.
format	article
author	Jiawei Yang Mingxu Zhang Qiuyi Song Siqi Li Xiulan Zhao Liping Kan Siquan Zhu
author_facet	Jiawei Yang Mingxu Zhang Qiuyi Song Siqi Li Xiulan Zhao Liping Kan Siquan Zhu
author_sort	Jiawei Yang
title	Integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma
title_short	Integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma
title_full	Integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma
title_fullStr	Integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma
title_full_unstemmed	Integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma
title_sort	integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma
publisher	BMC
publishDate	2021
url	https://doaj.org/article/b15b702b461147b1af5a9357944be66f
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_version_	1718408220171567104

Integrating network pharmacological and experimental models to investigate the therapeutic effects of baicalein in glaucoma

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