Docosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment

Amr Alaarg,1,2 Nan Yeun Jordan,1 Johan JF Verhoef,1 Josbert M Metselaar,2,3 Gert Storm,1,2 Robbert J Kok1 1Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, 2Department of Biomaterials Science and Technology, Institute for Biomedical Technology...

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Autores principales:	Alaarg A, Jordan NY, Verhoef JJF, Metselaar JM, Storm G, Kok RJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Nanomedicine Liposomes Docosahexanoic acid PUFA Inflammation Cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b15d774cf888422981367685c90fbb00
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spelling	oai:doaj.org-article:b15d774cf888422981367685c90fbb002021-12-02T07:36:53ZDocosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment1178-2013https://doaj.org/article/b15d774cf888422981367685c90fbb002016-10-01T00:00:00Zhttps://www.dovepress.com/docosahexaenoic-acid-liposomes-for-targeting-chronic-inflammatory-dise-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Amr Alaarg,1,2 Nan Yeun Jordan,1 Johan JF Verhoef,1 Josbert M Metselaar,2,3 Gert Storm,1,2 Robbert J Kok1 1Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, 2Department of Biomaterials Science and Technology, Institute for Biomedical Technology and Technical Medicine (MIRA), University of Twente, Enschede, the Netherlands; 3Department of Experimental Molecular Imaging, University Clinic and Helmholtz Institute for Biomedical Engineering, RWTH-Aachen University, Aachen, Germany Abstract: Inflammation, oxidative stress, and uncontrolled cell proliferation are common key features of chronic inflammatory diseases, such as atherosclerosis and cancer. ω3 polyunsaturated fatty acids (PUFAs; also known as omega3 fatty acids or fish oil) have beneficial effects against inflammation upon dietary consumption. However, these effects cannot be fully exploited unless diets are enriched with high concentrations of fish oil supplements over long periods of time. Here, a nanomedicine-based approach is presented for delivering effective levels of PUFAs to inflammatory cells. Nanoparticles are internalized by immune cells, and hence can adequately deliver bioactive lipids into these target cells. The ω3 FA docosahexaenoic acid was formulated into liposomes (ω-liposomes), and evaluated for anti-inflammatory effects in different types of immune cells. ω-Liposomes strongly inhibited the release of reactive oxygen species and reactive nitrogen species from human neutrophils and murine macrophages, and also inhibited the production of the proinflammatory cytokines TNFα and MCP1. Moreover, ω-liposomes inhibited tumor-cell proliferation when evaluated in FaDu head and neck squamous carcinoma and 4T1 breast cancer cells in in vitro cultures. We propose that ω-liposomes are a promising nanonutraceutical formulation for intravenous delivery of fish oil FAs, which may be beneficial in the treatment of inflammatory disorders and cancer. Keywords: nanomedicine, PUFA, inflammation, cancer, fish oil, deliveryAlaarg AJordan NYVerhoef JJFMetselaar JMStorm GKok RJDove Medical PressarticleNanomedicineLiposomesDocosahexanoic acidPUFAInflammationCancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5027-5040 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanomedicine Liposomes Docosahexanoic acid PUFA Inflammation Cancer Medicine (General) R5-920
spellingShingle	Nanomedicine Liposomes Docosahexanoic acid PUFA Inflammation Cancer Medicine (General) R5-920 Alaarg A Jordan NY Verhoef JJF Metselaar JM Storm G Kok RJ Docosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment
description	Amr Alaarg,1,2 Nan Yeun Jordan,1 Johan JF Verhoef,1 Josbert M Metselaar,2,3 Gert Storm,1,2 Robbert J Kok1 1Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, 2Department of Biomaterials Science and Technology, Institute for Biomedical Technology and Technical Medicine (MIRA), University of Twente, Enschede, the Netherlands; 3Department of Experimental Molecular Imaging, University Clinic and Helmholtz Institute for Biomedical Engineering, RWTH-Aachen University, Aachen, Germany Abstract: Inflammation, oxidative stress, and uncontrolled cell proliferation are common key features of chronic inflammatory diseases, such as atherosclerosis and cancer. ω3 polyunsaturated fatty acids (PUFAs; also known as omega3 fatty acids or fish oil) have beneficial effects against inflammation upon dietary consumption. However, these effects cannot be fully exploited unless diets are enriched with high concentrations of fish oil supplements over long periods of time. Here, a nanomedicine-based approach is presented for delivering effective levels of PUFAs to inflammatory cells. Nanoparticles are internalized by immune cells, and hence can adequately deliver bioactive lipids into these target cells. The ω3 FA docosahexaenoic acid was formulated into liposomes (ω-liposomes), and evaluated for anti-inflammatory effects in different types of immune cells. ω-Liposomes strongly inhibited the release of reactive oxygen species and reactive nitrogen species from human neutrophils and murine macrophages, and also inhibited the production of the proinflammatory cytokines TNFα and MCP1. Moreover, ω-liposomes inhibited tumor-cell proliferation when evaluated in FaDu head and neck squamous carcinoma and 4T1 breast cancer cells in in vitro cultures. We propose that ω-liposomes are a promising nanonutraceutical formulation for intravenous delivery of fish oil FAs, which may be beneficial in the treatment of inflammatory disorders and cancer. Keywords: nanomedicine, PUFA, inflammation, cancer, fish oil, delivery
format	article
author	Alaarg A Jordan NY Verhoef JJF Metselaar JM Storm G Kok RJ
author_facet	Alaarg A Jordan NY Verhoef JJF Metselaar JM Storm G Kok RJ
author_sort	Alaarg A
title	Docosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment
title_short	Docosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment
title_full	Docosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment
title_fullStr	Docosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment
title_full_unstemmed	Docosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment
title_sort	docosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/b15d774cf888422981367685c90fbb00
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Docosahexaenoic acid liposomes for targeting chronic inflammatory diseases and cancer: an in vitro assessment

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