PbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit

Abstract Pear [Pyrus bretschneideri cv. Dangshan Su] fruit quality is not always satisfactory owing to the presence of stone cells, and lignin is the main component of stone cells in pear fruits. Caffeoyl shikimate esterase (CSE) is a key enzyme in the lignin biosynthesis. Although CSE-like genes ha...

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Autores principales:	Jiahui Xu, Xingyu Tao, Zhihua Xie, Xin Gong, Kaijie Qi, Shaoling Zhang, Katsuhiro Shiratake, Shutian Tao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b15dd7a596054a66bdf4a078c07ac41e
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spelling	oai:doaj.org-article:b15dd7a596054a66bdf4a078c07ac41e2021-12-02T15:38:10ZPbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit10.1038/s41598-021-88825-02045-2322https://doaj.org/article/b15dd7a596054a66bdf4a078c07ac41e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88825-0https://doaj.org/toc/2045-2322Abstract Pear [Pyrus bretschneideri cv. Dangshan Su] fruit quality is not always satisfactory owing to the presence of stone cells, and lignin is the main component of stone cells in pear fruits. Caffeoyl shikimate esterase (CSE) is a key enzyme in the lignin biosynthesis. Although CSE-like genes have been isolated from a variety of plant species, their orthologs are not characterized in pear. In this study, the CSE gene family (PbCSE) from P. bretschneideri was identified. According to the physiological data and quantitative RT-PCR (qRT-PCR), PbCSE1 was associated with lignin deposition and stone cell formation. The overexpression of PbCSE1 increased the lignin content in pear fruits. Relative to wild-type (WT) Arabidopsis, the overexpression of PbCSE1 delayed growth, increased the lignin deposition and lignin content in stems. Simultaneously, the expression of lignin biosynthetic genes were also increased in pear fruits and Arabidopsis. These results demonstrated that PbCSE1 plays an important role in cell lignification and will provide a potential molecular strategy to improve the quality of pear fruits.Jiahui XuXingyu TaoZhihua XieXin GongKaijie QiShaoling ZhangKatsuhiro ShiratakeShutian TaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiahui Xu Xingyu Tao Zhihua Xie Xin Gong Kaijie Qi Shaoling Zhang Katsuhiro Shiratake Shutian Tao PbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit
description	Abstract Pear [Pyrus bretschneideri cv. Dangshan Su] fruit quality is not always satisfactory owing to the presence of stone cells, and lignin is the main component of stone cells in pear fruits. Caffeoyl shikimate esterase (CSE) is a key enzyme in the lignin biosynthesis. Although CSE-like genes have been isolated from a variety of plant species, their orthologs are not characterized in pear. In this study, the CSE gene family (PbCSE) from P. bretschneideri was identified. According to the physiological data and quantitative RT-PCR (qRT-PCR), PbCSE1 was associated with lignin deposition and stone cell formation. The overexpression of PbCSE1 increased the lignin content in pear fruits. Relative to wild-type (WT) Arabidopsis, the overexpression of PbCSE1 delayed growth, increased the lignin deposition and lignin content in stems. Simultaneously, the expression of lignin biosynthetic genes were also increased in pear fruits and Arabidopsis. These results demonstrated that PbCSE1 plays an important role in cell lignification and will provide a potential molecular strategy to improve the quality of pear fruits.
format	article
author	Jiahui Xu Xingyu Tao Zhihua Xie Xin Gong Kaijie Qi Shaoling Zhang Katsuhiro Shiratake Shutian Tao
author_facet	Jiahui Xu Xingyu Tao Zhihua Xie Xin Gong Kaijie Qi Shaoling Zhang Katsuhiro Shiratake Shutian Tao
author_sort	Jiahui Xu
title	PbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit
title_short	PbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit
title_full	PbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit
title_fullStr	PbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit
title_full_unstemmed	PbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit
title_sort	pbcse1 promotes lignification during stone cell development in pear (pyrus bretschneideri) fruit
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b15dd7a596054a66bdf4a078c07ac41e
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PbCSE1 promotes lignification during stone cell development in pear (Pyrus bretschneideri) fruit

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