Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC

Most of the active pharmaceutical ingredients in the pharmaceutical world are obtained from natural ingredients, one of which is white pepper. White pepper seeds (Piper nigrum L.) contain the main alkaloid compound piperine, which has a broad spectrum pharmacological effect. This research aimed to i...

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Autores principales:	Nindya Kusumorini, Akhmad Kharis Nugroho, Suwijiyo Pramono, Ronny Martien
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	white pepper, active pharmaceutical ingredient (api), nmr, piperine antioxidant Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/b16a4a6dc33e4f598404e5d3d604d904
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spelling	oai:doaj.org-article:b16a4a6dc33e4f598404e5d3d604d9042021-11-15T06:08:46ZDevelopment of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC2338-94272338-948610.22146/ijp.866https://doaj.org/article/b16a4a6dc33e4f598404e5d3d604d9042021-06-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/866https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Most of the active pharmaceutical ingredients in the pharmaceutical world are obtained from natural ingredients, one of which is white pepper. White pepper seeds (Piper nigrum L.) contain the main alkaloid compound piperine, which has a broad spectrum pharmacological effect. This research aimed to isolate the piperine compound from white pepper seeds to make piperine more economical, simple, and effective so that it can be applied to large scale production in the pharmaceutical industry. The method of extracting and purifying piperine compounds was carried out using n-hexane and cyclohexane. The results of the isolated piperine were tested for their purity with a set of melting point apparatus, bidimensional TLC, and HPLC. The structure compound was then analyzed using FTIR and NMR. Furthermore, the isolated piperine was tested for its antioxidant activities using DPPH, ABTS, and FRAP. Cyclohexane was successfully used to remove the resinous matter from the n-hexane extract of white pepper to produce isolated piperine with 97.24% of purity. The results of the melting point, FTIR, 1H-NMR, and 12C-NMR of the isolated piperine were similar to that of the piperine reference substance and the literature review. The isolated piperine has weak antioxidant activities in the ABTS and FRAP test with the results of 15.25 ± 0.004 mM and 10.53 mol TE/g of the sample, respectively.Nindya KusumoriniAkhmad Kharis NugrohoSuwijiyo PramonoRonny MartienUniversitas Gadjah Madaarticlewhite pepper, active pharmaceutical ingredient (api), nmr, piperine antioxidantPharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 158-165 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	white pepper, active pharmaceutical ingredient (api), nmr, piperine antioxidant Pharmacy and materia medica RS1-441
spellingShingle	white pepper, active pharmaceutical ingredient (api), nmr, piperine antioxidant Pharmacy and materia medica RS1-441 Nindya Kusumorini Akhmad Kharis Nugroho Suwijiyo Pramono Ronny Martien Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC
description	Most of the active pharmaceutical ingredients in the pharmaceutical world are obtained from natural ingredients, one of which is white pepper. White pepper seeds (Piper nigrum L.) contain the main alkaloid compound piperine, which has a broad spectrum pharmacological effect. This research aimed to isolate the piperine compound from white pepper seeds to make piperine more economical, simple, and effective so that it can be applied to large scale production in the pharmaceutical industry. The method of extracting and purifying piperine compounds was carried out using n-hexane and cyclohexane. The results of the isolated piperine were tested for their purity with a set of melting point apparatus, bidimensional TLC, and HPLC. The structure compound was then analyzed using FTIR and NMR. Furthermore, the isolated piperine was tested for its antioxidant activities using DPPH, ABTS, and FRAP. Cyclohexane was successfully used to remove the resinous matter from the n-hexane extract of white pepper to produce isolated piperine with 97.24% of purity. The results of the melting point, FTIR, 1H-NMR, and 12C-NMR of the isolated piperine were similar to that of the piperine reference substance and the literature review. The isolated piperine has weak antioxidant activities in the ABTS and FRAP test with the results of 15.25 ± 0.004 mM and 10.53 mol TE/g of the sample, respectively.
format	article
author	Nindya Kusumorini Akhmad Kharis Nugroho Suwijiyo Pramono Ronny Martien
author_facet	Nindya Kusumorini Akhmad Kharis Nugroho Suwijiyo Pramono Ronny Martien
author_sort	Nindya Kusumorini
title	Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC
title_short	Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC
title_full	Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC
title_fullStr	Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC
title_full_unstemmed	Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC
title_sort	development of new isolation and quantification method of piperine from white pepper seeds (piper nigrum l) using a validated hplc
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/b16a4a6dc33e4f598404e5d3d604d904
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Development of New Isolation and Quantification Method of Piperine from White Pepper Seeds (Piper Nigrum L) Using A Validated HPLC

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