Disrupting quorum sensing alters social interactions in Chromobacterium violaceum

Abstract Quorum sensing (QS) is a communication system used by bacteria to coordinate a wide panel of biological functions in a cell density-dependent manner. The Gram-negative Chromobacterium violaceum has previously been shown to use an acyl-homoserine lactone (AHL)-based QS to regulate various be...

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Autores principales:	Sonia Mion, Nathan Carriot, Julien Lopez, Laure Plener, Annick Ortalo-Magné, Eric Chabrière, Gérald Culioli, David Daudé
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/b16df5e04cb14885bbd5af536c37fd89
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spelling	oai:doaj.org-article:b16df5e04cb14885bbd5af536c37fd892021-12-02T13:39:35ZDisrupting quorum sensing alters social interactions in Chromobacterium violaceum10.1038/s41522-021-00211-w2055-5008https://doaj.org/article/b16df5e04cb14885bbd5af536c37fd892021-04-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00211-whttps://doaj.org/toc/2055-5008Abstract Quorum sensing (QS) is a communication system used by bacteria to coordinate a wide panel of biological functions in a cell density-dependent manner. The Gram-negative Chromobacterium violaceum has previously been shown to use an acyl-homoserine lactone (AHL)-based QS to regulate various behaviors, including the production of proteases, hydrogen cyanide, or antimicrobial compounds such as violacein. By using combined metabolomic and proteomic approaches, we demonstrated that QS modulates the production of antimicrobial and toxic compounds in C. violaceum ATCC 12472. We provided the first evidence of anisomycin antibiotic production by this strain as well as evidence of its regulation by QS and identified new AHLs produced by C. violaceum ATCC 12472. Furthermore, we demonstrated that targeting AHLs with lactonase leads to major QS disruption yielding significant molecular and phenotypic changes. These modifications resulted in drastic changes in social interactions between C. violaceum and a Gram-positive bacterium (Bacillus cereus), a yeast (Saccharomyces cerevisiae), immune cells (murine macrophages), and an animal model (planarian Schmidtea mediterranea). These results underscored that AHL-based QS plays a key role in the capacity of C. violaceum to interact with micro- and macroorganisms and that quorum quenching can affect microbial population dynamics beyond AHL-producing bacteria and Gram-negative bacteria.Sonia MionNathan CarriotJulien LopezLaure PlenerAnnick Ortalo-MagnéEric ChabrièreGérald CulioliDavid DaudéNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Sonia Mion Nathan Carriot Julien Lopez Laure Plener Annick Ortalo-Magné Eric Chabrière Gérald Culioli David Daudé Disrupting quorum sensing alters social interactions in Chromobacterium violaceum
description	Abstract Quorum sensing (QS) is a communication system used by bacteria to coordinate a wide panel of biological functions in a cell density-dependent manner. The Gram-negative Chromobacterium violaceum has previously been shown to use an acyl-homoserine lactone (AHL)-based QS to regulate various behaviors, including the production of proteases, hydrogen cyanide, or antimicrobial compounds such as violacein. By using combined metabolomic and proteomic approaches, we demonstrated that QS modulates the production of antimicrobial and toxic compounds in C. violaceum ATCC 12472. We provided the first evidence of anisomycin antibiotic production by this strain as well as evidence of its regulation by QS and identified new AHLs produced by C. violaceum ATCC 12472. Furthermore, we demonstrated that targeting AHLs with lactonase leads to major QS disruption yielding significant molecular and phenotypic changes. These modifications resulted in drastic changes in social interactions between C. violaceum and a Gram-positive bacterium (Bacillus cereus), a yeast (Saccharomyces cerevisiae), immune cells (murine macrophages), and an animal model (planarian Schmidtea mediterranea). These results underscored that AHL-based QS plays a key role in the capacity of C. violaceum to interact with micro- and macroorganisms and that quorum quenching can affect microbial population dynamics beyond AHL-producing bacteria and Gram-negative bacteria.
format	article
author	Sonia Mion Nathan Carriot Julien Lopez Laure Plener Annick Ortalo-Magné Eric Chabrière Gérald Culioli David Daudé
author_facet	Sonia Mion Nathan Carriot Julien Lopez Laure Plener Annick Ortalo-Magné Eric Chabrière Gérald Culioli David Daudé
author_sort	Sonia Mion
title	Disrupting quorum sensing alters social interactions in Chromobacterium violaceum
title_short	Disrupting quorum sensing alters social interactions in Chromobacterium violaceum
title_full	Disrupting quorum sensing alters social interactions in Chromobacterium violaceum
title_fullStr	Disrupting quorum sensing alters social interactions in Chromobacterium violaceum
title_full_unstemmed	Disrupting quorum sensing alters social interactions in Chromobacterium violaceum
title_sort	disrupting quorum sensing alters social interactions in chromobacterium violaceum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b16df5e04cb14885bbd5af536c37fd89
work_keys_str_mv	AT soniamion disruptingquorumsensingalterssocialinteractionsinchromobacteriumviolaceum AT nathancarriot disruptingquorumsensingalterssocialinteractionsinchromobacteriumviolaceum AT julienlopez disruptingquorumsensingalterssocialinteractionsinchromobacteriumviolaceum AT laureplener disruptingquorumsensingalterssocialinteractionsinchromobacteriumviolaceum AT annickortalomagne disruptingquorumsensingalterssocialinteractionsinchromobacteriumviolaceum AT ericchabriere disruptingquorumsensingalterssocialinteractionsinchromobacteriumviolaceum AT geraldculioli disruptingquorumsensingalterssocialinteractionsinchromobacteriumviolaceum AT daviddaude disruptingquorumsensingalterssocialinteractionsinchromobacteriumviolaceum
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Disrupting quorum sensing alters social interactions in Chromobacterium violaceum

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