Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies.

MicroRNAs (miRNAs) are stable, small non-coding RNAs that modulate many downstream target genes. Recently, circulating miRNAs have been detected in various body fluids and within exosomes, prompting their evaluation as candidate biomarkers of diseases, especially cancer. Kaposi's sarcoma (KS) i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pauline E Chugh, Sang-Hoon Sin, Sezgin Ozgur, David H Henry, Prema Menezes, Jack Griffith, Joseph J Eron, Blossom Damania, Dirk P Dittmer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b16e1b81183045c0b0f5e83a301ce1a8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b16e1b81183045c0b0f5e83a301ce1a8
record_format	dspace
spelling	oai:doaj.org-article:b16e1b81183045c0b0f5e83a301ce1a82021-11-18T06:05:22ZSystemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies.1553-73661553-737410.1371/journal.ppat.1003484https://doaj.org/article/b16e1b81183045c0b0f5e83a301ce1a82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874201/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374MicroRNAs (miRNAs) are stable, small non-coding RNAs that modulate many downstream target genes. Recently, circulating miRNAs have been detected in various body fluids and within exosomes, prompting their evaluation as candidate biomarkers of diseases, especially cancer. Kaposi's sarcoma (KS) is the most common AIDS-associated cancer and remains prevalent despite Highly Active Anti-Retroviral Therapy (HAART). KS is caused by KS-associated herpesvirus (KSHV), a gamma herpesvirus also associated with Primary Effusion Lymphoma (PEL). We sought to determine the host and viral circulating miRNAs in plasma, pleural fluid or serum from patients with the KSHV-associated malignancies KS and PEL and from two mouse models of KS. Both KSHV-encoded miRNAs and host miRNAs, including members of the miR-17-92 cluster, were detectable within patient exosomes and circulating miRNA profiles from KSHV mouse models. Further characterization revealed a subset of miRNAs that seemed to be preferentially incorporated into exosomes. Gene ontology analysis of signature exosomal miRNA targets revealed several signaling pathways that are known to be important in KSHV pathogenesis. Functional analysis of endothelial cells exposed to patient-derived exosomes demonstrated enhanced cell migration and IL-6 secretion. This suggests that exosomes derived from KSHV-associated malignancies are functional and contain a distinct subset of miRNAs. These could represent candidate biomarkers of disease and may contribute to the paracrine phenotypes that are a characteristic of KS.Pauline E ChughSang-Hoon SinSezgin OzgurDavid H HenryPrema MenezesJack GriffithJoseph J EronBlossom DamaniaDirk P DittmerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 7, p e1003484 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Pauline E Chugh Sang-Hoon Sin Sezgin Ozgur David H Henry Prema Menezes Jack Griffith Joseph J Eron Blossom Damania Dirk P Dittmer Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies.
description	MicroRNAs (miRNAs) are stable, small non-coding RNAs that modulate many downstream target genes. Recently, circulating miRNAs have been detected in various body fluids and within exosomes, prompting their evaluation as candidate biomarkers of diseases, especially cancer. Kaposi's sarcoma (KS) is the most common AIDS-associated cancer and remains prevalent despite Highly Active Anti-Retroviral Therapy (HAART). KS is caused by KS-associated herpesvirus (KSHV), a gamma herpesvirus also associated with Primary Effusion Lymphoma (PEL). We sought to determine the host and viral circulating miRNAs in plasma, pleural fluid or serum from patients with the KSHV-associated malignancies KS and PEL and from two mouse models of KS. Both KSHV-encoded miRNAs and host miRNAs, including members of the miR-17-92 cluster, were detectable within patient exosomes and circulating miRNA profiles from KSHV mouse models. Further characterization revealed a subset of miRNAs that seemed to be preferentially incorporated into exosomes. Gene ontology analysis of signature exosomal miRNA targets revealed several signaling pathways that are known to be important in KSHV pathogenesis. Functional analysis of endothelial cells exposed to patient-derived exosomes demonstrated enhanced cell migration and IL-6 secretion. This suggests that exosomes derived from KSHV-associated malignancies are functional and contain a distinct subset of miRNAs. These could represent candidate biomarkers of disease and may contribute to the paracrine phenotypes that are a characteristic of KS.
format	article
author	Pauline E Chugh Sang-Hoon Sin Sezgin Ozgur David H Henry Prema Menezes Jack Griffith Joseph J Eron Blossom Damania Dirk P Dittmer
author_facet	Pauline E Chugh Sang-Hoon Sin Sezgin Ozgur David H Henry Prema Menezes Jack Griffith Joseph J Eron Blossom Damania Dirk P Dittmer
author_sort	Pauline E Chugh
title	Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies.
title_short	Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies.
title_full	Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies.
title_fullStr	Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies.
title_full_unstemmed	Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies.
title_sort	systemically circulating viral and tumor-derived micrornas in kshv-associated malignancies.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b16e1b81183045c0b0f5e83a301ce1a8
work_keys_str_mv	AT paulineechugh systemicallycirculatingviralandtumorderivedmicrornasinkshvassociatedmalignancies AT sanghoonsin systemicallycirculatingviralandtumorderivedmicrornasinkshvassociatedmalignancies AT sezginozgur systemicallycirculatingviralandtumorderivedmicrornasinkshvassociatedmalignancies AT davidhhenry systemicallycirculatingviralandtumorderivedmicrornasinkshvassociatedmalignancies AT premamenezes systemicallycirculatingviralandtumorderivedmicrornasinkshvassociatedmalignancies AT jackgriffith systemicallycirculatingviralandtumorderivedmicrornasinkshvassociatedmalignancies AT josephjeron systemicallycirculatingviralandtumorderivedmicrornasinkshvassociatedmalignancies AT blossomdamania systemicallycirculatingviralandtumorderivedmicrornasinkshvassociatedmalignancies AT dirkpdittmer systemicallycirculatingviralandtumorderivedmicrornasinkshvassociatedmalignancies
_version_	1718424627154255872

Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies.

Ejemplares similares