Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles

Abstract To test the hypothesis that intrinsic renal scattered tubular cells (STC-like cells) contribute to repairing injured tubular epithelial cells (TEC) by releasing extracellular vesicle (EV). EV released from primary cultured pig STC-like cells were confirmed by electron microscopy. Antimycin-...

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Autores principales:	Xiangyu Zou, Soon Hyo Kwon, Kai Jiang, Christopher M. Ferguson, Amrutesh S. Puranik, Xiangyang Zhu, Lilach O. Lerman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1703d65fdf346acbab51e8e5012215a
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spelling	oai:doaj.org-article:b1703d65fdf346acbab51e8e5012215a2021-12-02T15:09:11ZRenal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles10.1038/s41598-018-19750-y2045-2322https://doaj.org/article/b1703d65fdf346acbab51e8e5012215a2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19750-yhttps://doaj.org/toc/2045-2322Abstract To test the hypothesis that intrinsic renal scattered tubular cells (STC-like cells) contribute to repairing injured tubular epithelial cells (TEC) by releasing extracellular vesicle (EV). EV released from primary cultured pig STC-like cells were confirmed by electron microscopy. Antimycin-A (AMA)-induced injured proximal TEC (PK1 cells) were co-cultured with STC-like cells, STC-like cells-derived EV, or EV-free conditioned-medium for 3 days. Cellular injury, oxidative stress and mitochondrial function were assessed. Transfer of mitochondria from STC-like cells to TEC was assessed using Mito-trackers, and their viability by mitochondrial membrane potential assays. STC-like cells-derived EV were intra-arterially injected into mice 2 weeks after induction of unilateral renal artery stenosis. Two weeks later, renal hemodynamics were studied using magnetic-resonance-imaging, and renal fibrosis assessed ex-vivo. Cultured STC-like cells released EV that were uptaken by TEC. A protective effect conferred by STC-like cells in AMA-induced TEC injury was partly mimicked by their EV. Furthermore, STC-like cells-EV carried and transferred mitochondrial material to injured TEC, which partly restored mitochondrial function. In vivo, STC-like cells-derived EV engrafted in the stenotic kidney, and improved its perfusion and oxygenation. STC-like cells-EV exert protective effects on injured tubular cells in vitro and in vivo, partly by transferring STC-like cells mitochondria, which remain at least partly functional in recipient TEC.Xiangyu ZouSoon Hyo KwonKai JiangChristopher M. FergusonAmrutesh S. PuranikXiangyang ZhuLilach O. LermanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiangyu Zou Soon Hyo Kwon Kai Jiang Christopher M. Ferguson Amrutesh S. Puranik Xiangyang Zhu Lilach O. Lerman Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles
description	Abstract To test the hypothesis that intrinsic renal scattered tubular cells (STC-like cells) contribute to repairing injured tubular epithelial cells (TEC) by releasing extracellular vesicle (EV). EV released from primary cultured pig STC-like cells were confirmed by electron microscopy. Antimycin-A (AMA)-induced injured proximal TEC (PK1 cells) were co-cultured with STC-like cells, STC-like cells-derived EV, or EV-free conditioned-medium for 3 days. Cellular injury, oxidative stress and mitochondrial function were assessed. Transfer of mitochondria from STC-like cells to TEC was assessed using Mito-trackers, and their viability by mitochondrial membrane potential assays. STC-like cells-derived EV were intra-arterially injected into mice 2 weeks after induction of unilateral renal artery stenosis. Two weeks later, renal hemodynamics were studied using magnetic-resonance-imaging, and renal fibrosis assessed ex-vivo. Cultured STC-like cells released EV that were uptaken by TEC. A protective effect conferred by STC-like cells in AMA-induced TEC injury was partly mimicked by their EV. Furthermore, STC-like cells-EV carried and transferred mitochondrial material to injured TEC, which partly restored mitochondrial function. In vivo, STC-like cells-derived EV engrafted in the stenotic kidney, and improved its perfusion and oxygenation. STC-like cells-EV exert protective effects on injured tubular cells in vitro and in vivo, partly by transferring STC-like cells mitochondria, which remain at least partly functional in recipient TEC.
format	article
author	Xiangyu Zou Soon Hyo Kwon Kai Jiang Christopher M. Ferguson Amrutesh S. Puranik Xiangyang Zhu Lilach O. Lerman
author_facet	Xiangyu Zou Soon Hyo Kwon Kai Jiang Christopher M. Ferguson Amrutesh S. Puranik Xiangyang Zhu Lilach O. Lerman
author_sort	Xiangyu Zou
title	Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles
title_short	Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles
title_full	Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles
title_fullStr	Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles
title_full_unstemmed	Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles
title_sort	renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b1703d65fdf346acbab51e8e5012215a
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Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles

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