Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.

Processing bodies (PBs) and stress granules (SGs) are related, cytoplasmic RNA-protein complexes that contribute to post-transcriptional gene regulation in all eukaryotic cells. Both structures contain translationally repressed mRNAs and several proteins involved in silencing, stabilization or degra...

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Autores principales:	Aris Rousakis, Anna Vlanti, Fivos Borbolis, Fani Roumelioti, Marianna Kapetanou, Popi Syntichaki
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1711fcb379a45cda361e306de236f2b
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spelling	oai:doaj.org-article:b1711fcb379a45cda361e306de236f2b2021-11-25T06:07:10ZDiverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.1932-620310.1371/journal.pone.0103365https://doaj.org/article/b1711fcb379a45cda361e306de236f2b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25061667/?tool=EBIhttps://doaj.org/toc/1932-6203Processing bodies (PBs) and stress granules (SGs) are related, cytoplasmic RNA-protein complexes that contribute to post-transcriptional gene regulation in all eukaryotic cells. Both structures contain translationally repressed mRNAs and several proteins involved in silencing, stabilization or degradation of mRNAs, especially under environmental stress. Here, we monitored the dynamic formation of PBs and SGs, in somatic cells of adult worms, using fluorescently tagged protein markers of each complex. Both complexes were accumulated in response to various stress conditions, but distinct modes of SG formation were induced, depending on the insult. We also observed an age-dependent accumulation of PBs but not of SGs. We further showed that direct alterations in PB-related genes can influence aging and normal stress responses, beyond their developmental role. In addition, disruption of SG-related genes had diverse effects on development, fertility, lifespan and stress resistance of worms. Our work therefore underlines the important roles of mRNA metabolism factors in several vital cellular processes and provides insight into their diverse functions in a multicellular organism.Aris RousakisAnna VlantiFivos BorbolisFani RoumeliotiMarianna KapetanouPopi SyntichakiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e103365 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aris Rousakis Anna Vlanti Fivos Borbolis Fani Roumelioti Marianna Kapetanou Popi Syntichaki Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.
description	Processing bodies (PBs) and stress granules (SGs) are related, cytoplasmic RNA-protein complexes that contribute to post-transcriptional gene regulation in all eukaryotic cells. Both structures contain translationally repressed mRNAs and several proteins involved in silencing, stabilization or degradation of mRNAs, especially under environmental stress. Here, we monitored the dynamic formation of PBs and SGs, in somatic cells of adult worms, using fluorescently tagged protein markers of each complex. Both complexes were accumulated in response to various stress conditions, but distinct modes of SG formation were induced, depending on the insult. We also observed an age-dependent accumulation of PBs but not of SGs. We further showed that direct alterations in PB-related genes can influence aging and normal stress responses, beyond their developmental role. In addition, disruption of SG-related genes had diverse effects on development, fertility, lifespan and stress resistance of worms. Our work therefore underlines the important roles of mRNA metabolism factors in several vital cellular processes and provides insight into their diverse functions in a multicellular organism.
format	article
author	Aris Rousakis Anna Vlanti Fivos Borbolis Fani Roumelioti Marianna Kapetanou Popi Syntichaki
author_facet	Aris Rousakis Anna Vlanti Fivos Borbolis Fani Roumelioti Marianna Kapetanou Popi Syntichaki
author_sort	Aris Rousakis
title	Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.
title_short	Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.
title_full	Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.
title_fullStr	Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.
title_full_unstemmed	Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.
title_sort	diverse functions of mrna metabolism factors in stress defense and aging of caenorhabditis elegans.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b1711fcb379a45cda361e306de236f2b
work_keys_str_mv	AT arisrousakis diversefunctionsofmrnametabolismfactorsinstressdefenseandagingofcaenorhabditiselegans AT annavlanti diversefunctionsofmrnametabolismfactorsinstressdefenseandagingofcaenorhabditiselegans AT fivosborbolis diversefunctionsofmrnametabolismfactorsinstressdefenseandagingofcaenorhabditiselegans AT faniroumelioti diversefunctionsofmrnametabolismfactorsinstressdefenseandagingofcaenorhabditiselegans AT mariannakapetanou diversefunctionsofmrnametabolismfactorsinstressdefenseandagingofcaenorhabditiselegans AT popisyntichaki diversefunctionsofmrnametabolismfactorsinstressdefenseandagingofcaenorhabditiselegans
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Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans.

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