A Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker

Current assays for <i>Clostridioides difficile</i> in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals. We designed a more rapid point-of-care assay featuring a “turn-on” bioluminescent readout of a <i...

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Autores principales:	Kevin K. Ng, Zachary E. Reinert, Jeroen Corver, Danica Resurreccion, Paul J. Hensbergen, Jennifer A. Prescher
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Clostridioides difficile</i> NanoLuc point-of-care device bioluminescent sensor protease sensor PPEP-1 Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/b171d600edd042be9f3169e0703c8145
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spelling	oai:doaj.org-article:b171d600edd042be9f3169e0703c81452021-11-25T18:56:50ZA Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker10.3390/s212274851424-8220https://doaj.org/article/b171d600edd042be9f3169e0703c81452021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/22/7485https://doaj.org/toc/1424-8220Current assays for <i>Clostridioides difficile</i> in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals. We designed a more rapid point-of-care assay featuring a “turn-on” bioluminescent readout of a <i>C. difficile</i>-specific protease, PPEP-1. NanoLuc, a bright and stable luciferase, was “caged” with a PPEP-1-responsive peptide tail that inhibited luminescence. Upon proteolytic cleavage, the peptide was released and NanoLuc activity was restored, providing a visible readout. The bioluminescent sensor detected PPEP-1 concentrations as low as 10 nM. Sensor uncaging was achieved within minutes, and signal was captured using a digital camera. Importantly, the sensor was also functional at ambient temperature and compatible with fecal material, suggesting that it can be readily deployed in a variety of settings.Kevin K. NgZachary E. ReinertJeroen CorverDanica ResurreccionPaul J. HensbergenJennifer A. PrescherMDPI AGarticle<i>Clostridioides difficile</i>NanoLucpoint-of-care devicebioluminescent sensorprotease sensorPPEP-1Chemical technologyTP1-1185ENSensors, Vol 21, Iss 7485, p 7485 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Clostridioides difficile</i> NanoLuc point-of-care device bioluminescent sensor protease sensor PPEP-1 Chemical technology TP1-1185
spellingShingle	<i>Clostridioides difficile</i> NanoLuc point-of-care device bioluminescent sensor protease sensor PPEP-1 Chemical technology TP1-1185 Kevin K. Ng Zachary E. Reinert Jeroen Corver Danica Resurreccion Paul J. Hensbergen Jennifer A. Prescher A Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker
description	Current assays for <i>Clostridioides difficile</i> in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals. We designed a more rapid point-of-care assay featuring a “turn-on” bioluminescent readout of a <i>C. difficile</i>-specific protease, PPEP-1. NanoLuc, a bright and stable luciferase, was “caged” with a PPEP-1-responsive peptide tail that inhibited luminescence. Upon proteolytic cleavage, the peptide was released and NanoLuc activity was restored, providing a visible readout. The bioluminescent sensor detected PPEP-1 concentrations as low as 10 nM. Sensor uncaging was achieved within minutes, and signal was captured using a digital camera. Importantly, the sensor was also functional at ambient temperature and compatible with fecal material, suggesting that it can be readily deployed in a variety of settings.
format	article
author	Kevin K. Ng Zachary E. Reinert Jeroen Corver Danica Resurreccion Paul J. Hensbergen Jennifer A. Prescher
author_facet	Kevin K. Ng Zachary E. Reinert Jeroen Corver Danica Resurreccion Paul J. Hensbergen Jennifer A. Prescher
author_sort	Kevin K. Ng
title	A Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker
title_short	A Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker
title_full	A Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker
title_fullStr	A Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker
title_full_unstemmed	A Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker
title_sort	bioluminescent sensor for rapid detection of ppep-1, a <i>clostridioides difficile</i> biomarker
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b171d600edd042be9f3169e0703c8145
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A Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker

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