Integration of global signaling pathways, cAMP-PKA, MAPK and TOR in the regulation of FLO11.

The budding yeast, Saccharomyces cerevisiae, responds to various environmental cues by invoking specific adaptive mechanisms for their survival. Under nitrogen limitation, S. cerevisiae undergoes a dimorphic filamentous transition called pseudohyphae, which helps the cell to forage for nutrients and...

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Autores principales:	P K Vinod, Neelanjan Sengupta, P J Bhat, K V Venkatesh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b17680b80289437fbfc047690ca8959d
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spelling	oai:doaj.org-article:b17680b80289437fbfc047690ca8959d2021-11-25T06:13:17ZIntegration of global signaling pathways, cAMP-PKA, MAPK and TOR in the regulation of FLO11.1932-620310.1371/journal.pone.0001663https://doaj.org/article/b17680b80289437fbfc047690ca8959d2008-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18301741/?tool=EBIhttps://doaj.org/toc/1932-6203The budding yeast, Saccharomyces cerevisiae, responds to various environmental cues by invoking specific adaptive mechanisms for their survival. Under nitrogen limitation, S. cerevisiae undergoes a dimorphic filamentous transition called pseudohyphae, which helps the cell to forage for nutrients and reach an environment conducive for growth. This transition is governed by a complex network of signaling pathways, namely cAMP-PKA, MAPK and TOR, which controls the transcriptional activation of FLO11, a flocculin gene that encodes a cell wall protein. However, little is known about how these pathways co-ordinate to govern the conversion of nutritional availability into gene expression. Here, we have analyzed an integrative network comprised of cAMP-PKA, MAPK and TOR pathways with respect to the availability of nitrogen source using experimental and steady state modeling approach. Our experiments demonstrate that the steady state expression of FLO11 was bistable over a range of inducing ammonium sulphate concentration based on the preculturing condition. We also show that yeast switched from FLO11 expression to accumulation of trehalose, a STRE response controlled by a transcriptional activator Msn2/4, with decrease in the inducing concentration to complete starvation. Steady state analysis of the integrative network revealed the relationship between the environment, signaling cascades and the expression of FLO11. We demonstrate that the double negative feedback loop in TOR pathway can elicit a bistable response, to differentiate between vegetative growth, filamentous growth and STRE response. Negative feedback on TOR pathway function to restrict the expression of FLO11 under nitrogen starved condition and also with re-addition of nitrogen to starved cells. In general, we show that these global signaling pathways respond with specific sensitivity to regulate the expression of FLO11 under nitrogen limitation. The holistic steady state modeling approach of the integrative network revealed how the global signaling pathways could differentiate between multiple phenotypes.P K VinodNeelanjan SenguptaP J BhatK V VenkateshPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 2, p e1663 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q P K Vinod Neelanjan Sengupta P J Bhat K V Venkatesh Integration of global signaling pathways, cAMP-PKA, MAPK and TOR in the regulation of FLO11.
description	The budding yeast, Saccharomyces cerevisiae, responds to various environmental cues by invoking specific adaptive mechanisms for their survival. Under nitrogen limitation, S. cerevisiae undergoes a dimorphic filamentous transition called pseudohyphae, which helps the cell to forage for nutrients and reach an environment conducive for growth. This transition is governed by a complex network of signaling pathways, namely cAMP-PKA, MAPK and TOR, which controls the transcriptional activation of FLO11, a flocculin gene that encodes a cell wall protein. However, little is known about how these pathways co-ordinate to govern the conversion of nutritional availability into gene expression. Here, we have analyzed an integrative network comprised of cAMP-PKA, MAPK and TOR pathways with respect to the availability of nitrogen source using experimental and steady state modeling approach. Our experiments demonstrate that the steady state expression of FLO11 was bistable over a range of inducing ammonium sulphate concentration based on the preculturing condition. We also show that yeast switched from FLO11 expression to accumulation of trehalose, a STRE response controlled by a transcriptional activator Msn2/4, with decrease in the inducing concentration to complete starvation. Steady state analysis of the integrative network revealed the relationship between the environment, signaling cascades and the expression of FLO11. We demonstrate that the double negative feedback loop in TOR pathway can elicit a bistable response, to differentiate between vegetative growth, filamentous growth and STRE response. Negative feedback on TOR pathway function to restrict the expression of FLO11 under nitrogen starved condition and also with re-addition of nitrogen to starved cells. In general, we show that these global signaling pathways respond with specific sensitivity to regulate the expression of FLO11 under nitrogen limitation. The holistic steady state modeling approach of the integrative network revealed how the global signaling pathways could differentiate between multiple phenotypes.
format	article
author	P K Vinod Neelanjan Sengupta P J Bhat K V Venkatesh
author_facet	P K Vinod Neelanjan Sengupta P J Bhat K V Venkatesh
author_sort	P K Vinod
title	Integration of global signaling pathways, cAMP-PKA, MAPK and TOR in the regulation of FLO11.
title_short	Integration of global signaling pathways, cAMP-PKA, MAPK and TOR in the regulation of FLO11.
title_full	Integration of global signaling pathways, cAMP-PKA, MAPK and TOR in the regulation of FLO11.
title_fullStr	Integration of global signaling pathways, cAMP-PKA, MAPK and TOR in the regulation of FLO11.
title_full_unstemmed	Integration of global signaling pathways, cAMP-PKA, MAPK and TOR in the regulation of FLO11.
title_sort	integration of global signaling pathways, camp-pka, mapk and tor in the regulation of flo11.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/b17680b80289437fbfc047690ca8959d
work_keys_str_mv	AT pkvinod integrationofglobalsignalingpathwayscamppkamapkandtorintheregulationofflo11 AT neelanjansengupta integrationofglobalsignalingpathwayscamppkamapkandtorintheregulationofflo11 AT pjbhat integrationofglobalsignalingpathwayscamppkamapkandtorintheregulationofflo11 AT kvvenkatesh integrationofglobalsignalingpathwayscamppkamapkandtorintheregulationofflo11
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Integration of global signaling pathways, cAMP-PKA, MAPK and TOR in the regulation of FLO11.

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