High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma

Abstract Due to the therapy resistance and frequent metastasis, pancreatic ductal adenocarcinoma(PDAC) remains one of the most malignant carcinoma. WNT7A, an important ligand of Wnt/β-catenin signaling pathways, has a controversial role in tumor development. The role of WNT7A in PDAC remains unclear...

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Autores principales:	Dong-jin Wu, Yong-sheng Jiang, Rui-zhe He, Ling-ye Tao, Min-wei Yang, Xue-liang Fu, Jiang-yu Yang, Kun Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Pancreatic Ductal Adenocarcinoma (PDAC) Wnt5a Expression Performed Transwell Assays PDAC Tissue PDAC Patients Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b177089439884fc18495fe7a563e2abb
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spelling	oai:doaj.org-article:b177089439884fc18495fe7a563e2abb2021-12-02T15:06:28ZHigh expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma10.1038/s41598-018-34094-32045-2322https://doaj.org/article/b177089439884fc18495fe7a563e2abb2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34094-3https://doaj.org/toc/2045-2322Abstract Due to the therapy resistance and frequent metastasis, pancreatic ductal adenocarcinoma(PDAC) remains one of the most malignant carcinoma. WNT7A, an important ligand of Wnt/β-catenin signaling pathways, has a controversial role in tumor development. The role of WNT7A in PDAC remains unclear. In this study, we analyzed the expression pattern of WNT7A at mRNA and protein levels. We found pancreatic cancer tissue demonstrated a significant high WNT7A expression compared with the adjacent non-tumor tissue and the expression of WNT7A positively correlates with poor prognosis and lymph node metastasis. Then, we performed transwell assays and wound healing assays in vitro and found that WNT7A promotes the migration capacity of cancer cells. Furthermore, we explored the underlying mechanism of the WNT7A inducing cell migration. Results showed that up-regulated WNT7A expression inducing higher expression of N-cadherin and lower expression of E-cadherin while the contrast result was shown in the WNT7A knock-down group, which suggested that WNT7A might contribute to an epithelial–mesenchymal transition. Finally, we found that the hypoxia culture condition remarkably increased the WNT7A expression. In conclusion, our work demonstrated that hypoxia induced high expression of WNT7A might promote the cell migration via enhancing the epithelial–mesenchymal transition in PDAC.Dong-jin WuYong-sheng JiangRui-zhe HeLing-ye TaoMin-wei YangXue-liang FuJiang-yu YangKun ZhuNature PortfolioarticlePancreatic Ductal Adenocarcinoma (PDAC)Wnt5a ExpressionPerformed Transwell AssaysPDAC TissuePDAC PatientsMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pancreatic Ductal Adenocarcinoma (PDAC) Wnt5a Expression Performed Transwell Assays PDAC Tissue PDAC Patients Medicine R Science Q
spellingShingle	Pancreatic Ductal Adenocarcinoma (PDAC) Wnt5a Expression Performed Transwell Assays PDAC Tissue PDAC Patients Medicine R Science Q Dong-jin Wu Yong-sheng Jiang Rui-zhe He Ling-ye Tao Min-wei Yang Xue-liang Fu Jiang-yu Yang Kun Zhu High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma
description	Abstract Due to the therapy resistance and frequent metastasis, pancreatic ductal adenocarcinoma(PDAC) remains one of the most malignant carcinoma. WNT7A, an important ligand of Wnt/β-catenin signaling pathways, has a controversial role in tumor development. The role of WNT7A in PDAC remains unclear. In this study, we analyzed the expression pattern of WNT7A at mRNA and protein levels. We found pancreatic cancer tissue demonstrated a significant high WNT7A expression compared with the adjacent non-tumor tissue and the expression of WNT7A positively correlates with poor prognosis and lymph node metastasis. Then, we performed transwell assays and wound healing assays in vitro and found that WNT7A promotes the migration capacity of cancer cells. Furthermore, we explored the underlying mechanism of the WNT7A inducing cell migration. Results showed that up-regulated WNT7A expression inducing higher expression of N-cadherin and lower expression of E-cadherin while the contrast result was shown in the WNT7A knock-down group, which suggested that WNT7A might contribute to an epithelial–mesenchymal transition. Finally, we found that the hypoxia culture condition remarkably increased the WNT7A expression. In conclusion, our work demonstrated that hypoxia induced high expression of WNT7A might promote the cell migration via enhancing the epithelial–mesenchymal transition in PDAC.
format	article
author	Dong-jin Wu Yong-sheng Jiang Rui-zhe He Ling-ye Tao Min-wei Yang Xue-liang Fu Jiang-yu Yang Kun Zhu
author_facet	Dong-jin Wu Yong-sheng Jiang Rui-zhe He Ling-ye Tao Min-wei Yang Xue-liang Fu Jiang-yu Yang Kun Zhu
author_sort	Dong-jin Wu
title	High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma
title_short	High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma
title_full	High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma
title_fullStr	High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma
title_full_unstemmed	High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma
title_sort	high expression of wnt7a predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/b177089439884fc18495fe7a563e2abb
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High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma

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