Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support

Abstract Hematological analysis is essential for patients who are supported by a mechanical circulatory support (MCS). The laboratory methods used to analyze blood components are conventional and accurate, but they require a mandatory turn-around-time for laboratory results, and because of toxic sub...

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Autores principales:	Dong Ah Shin, Jung Chan Lee, Heean Shin, Young-Jae Cho, Hee Chan Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b17ab5fb9f4e429595f3d938af2444c0
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spelling	oai:doaj.org-article:b17ab5fb9f4e429595f3d938af2444c02021-12-02T10:54:24ZPoint-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support10.1038/s41598-021-83327-52045-2322https://doaj.org/article/b17ab5fb9f4e429595f3d938af2444c02021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83327-5https://doaj.org/toc/2045-2322Abstract Hematological analysis is essential for patients who are supported by a mechanical circulatory support (MCS). The laboratory methods used to analyze blood components are conventional and accurate, but they require a mandatory turn-around-time for laboratory results, and because of toxic substances, can also be hazardous to analysis workers. Here, a simple and rapid point-of-care device is developed for the measurement of plasma free hemoglobin (PFHb) and hematocrit (Hct), based on colorimetry. The device consists of camera module, minimized centrifuge system, and the custom software that includes the motor control algorithm for the centrifuge system, and the image processing algorithm for measuring the color components of blood from the images. We show that our device measured PFHb with a detection limit of 0.75 mg/dL in the range of (0–100) mg/dL, and Hct with a detection limit of 2.14% in the range of (20–50)%. Our device had a high correlation with the measurement method generally used in clinical laboratories (PFHb R = 0.999, Hct R = 0.739), and the quantitative analysis resulted in precision of 1.44 mg/dL for PFHb value of 14.5 mg/dL, 1.36 mg/dL for PFHb value of 53 mg/dL, and 1.24% for Hct 30%. Also, the device can be measured without any pre-processing when compared to the clinical laboratory method, so results can be obtained within 5 min (about an 1 h for the clinical laboratory method). Therefore, we conclude that the device can be used for point-of-care measurement of PFHb and Hct for MCS.Dong Ah ShinJung Chan LeeHeean ShinYoung-Jae ChoHee Chan KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dong Ah Shin Jung Chan Lee Heean Shin Young-Jae Cho Hee Chan Kim Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support
description	Abstract Hematological analysis is essential for patients who are supported by a mechanical circulatory support (MCS). The laboratory methods used to analyze blood components are conventional and accurate, but they require a mandatory turn-around-time for laboratory results, and because of toxic substances, can also be hazardous to analysis workers. Here, a simple and rapid point-of-care device is developed for the measurement of plasma free hemoglobin (PFHb) and hematocrit (Hct), based on colorimetry. The device consists of camera module, minimized centrifuge system, and the custom software that includes the motor control algorithm for the centrifuge system, and the image processing algorithm for measuring the color components of blood from the images. We show that our device measured PFHb with a detection limit of 0.75 mg/dL in the range of (0–100) mg/dL, and Hct with a detection limit of 2.14% in the range of (20–50)%. Our device had a high correlation with the measurement method generally used in clinical laboratories (PFHb R = 0.999, Hct R = 0.739), and the quantitative analysis resulted in precision of 1.44 mg/dL for PFHb value of 14.5 mg/dL, 1.36 mg/dL for PFHb value of 53 mg/dL, and 1.24% for Hct 30%. Also, the device can be measured without any pre-processing when compared to the clinical laboratory method, so results can be obtained within 5 min (about an 1 h for the clinical laboratory method). Therefore, we conclude that the device can be used for point-of-care measurement of PFHb and Hct for MCS.
format	article
author	Dong Ah Shin Jung Chan Lee Heean Shin Young-Jae Cho Hee Chan Kim
author_facet	Dong Ah Shin Jung Chan Lee Heean Shin Young-Jae Cho Hee Chan Kim
author_sort	Dong Ah Shin
title	Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support
title_short	Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support
title_full	Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support
title_fullStr	Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support
title_full_unstemmed	Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support
title_sort	point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b17ab5fb9f4e429595f3d938af2444c0
work_keys_str_mv	AT dongahshin pointofcaretestingofplasmafreehemoglobinandhematocritformechanicalcirculatorysupport AT jungchanlee pointofcaretestingofplasmafreehemoglobinandhematocritformechanicalcirculatorysupport AT heeanshin pointofcaretestingofplasmafreehemoglobinandhematocritformechanicalcirculatorysupport AT youngjaecho pointofcaretestingofplasmafreehemoglobinandhematocritformechanicalcirculatorysupport AT heechankim pointofcaretestingofplasmafreehemoglobinandhematocritformechanicalcirculatorysupport
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Point-of-care testing of plasma free hemoglobin and hematocrit for mechanical circulatory support

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