Differences between the Leaf Mycobiome of Coffea arabica and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content

The study of microbes associated with the coffee tree has been gaining strength in recent years. In this work, we compared the leaf mycobiome of the traditional crop Coffea arabica with wild species Coffea racemosa and Coffea stenophylla using...

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Autores principales:	Leandro Pio de Sousa, Oliveiro Guerreiro Filho, Jorge Maurício Costa Mondego
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mycobiome caffeine chlorogenic acid <i>Coffea</i> sp. Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b17f091d3a794529842d47a9e76b0bb5
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spelling	oai:doaj.org-article:b17f091d3a794529842d47a9e76b0bb52021-11-25T18:24:55ZDifferences between the Leaf Mycobiome of <i>Coffea arabica</i> and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content10.3390/microorganisms91122962076-2607https://doaj.org/article/b17f091d3a794529842d47a9e76b0bb52021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2296https://doaj.org/toc/2076-2607The study of microbes associated with the coffee tree has been gaining strength in recent years. In this work, we compared the leaf mycobiome of the traditional crop <i>Coffea arabica</i> with wild species <i>Coffea racemosa</i> and <i>Coffea stenophylla</i> using ITS sequencing for qualitative information and real-time PCR for quantitative information, seeking to relate the mycobiomes with the content of caffeine and chlorogenic acid in leaves. <i>Dothideomycetes</i>, <i>Wallemiomycetes</i>, and <i>Tremellomycetes</i> are the dominant classes of fungi. The core leaf mycobiome among the three <i>Coffea</i> species is formed by <i>Hannaella</i>, <i>Cladosporium</i>, <i>Cryptococcus</i>, <i>Erythrobasidium,</i> and <i>Alternaria</i>. A network analysis showed that <i>Phoma</i>, an important <i>C. arabica</i> pathogen, is negatively related to six fungal species present in <i>C. racemosa</i> and <i>C. stenophylla</i> and absent in <i>C. arabica</i>. Finally, <i>C. arabica</i> have more than 35 times the concentration of caffeine and 2.5 times the concentration of chlorogenic acid than <i>C. stenophylla</i> and <i>C. racemosa</i>. The relationship between caffeine/chlorogenic acid content, the leaf mycobiome, and genotype pathogen resistance is discussed.Leandro Pio de SousaOliveiro Guerreiro FilhoJorge Maurício Costa MondegoMDPI AGarticlemycobiomecaffeinechlorogenic acid<i>Coffea</i> sp.Biology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2296, p 2296 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycobiome caffeine chlorogenic acid <i>Coffea</i> sp. Biology (General) QH301-705.5
spellingShingle	mycobiome caffeine chlorogenic acid <i>Coffea</i> sp. Biology (General) QH301-705.5 Leandro Pio de Sousa Oliveiro Guerreiro Filho Jorge Maurício Costa Mondego Differences between the Leaf Mycobiome of <i>Coffea arabica</i> and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content
description	The study of microbes associated with the coffee tree has been gaining strength in recent years. In this work, we compared the leaf mycobiome of the traditional crop <i>Coffea arabica</i> with wild species <i>Coffea racemosa</i> and <i>Coffea stenophylla</i> using ITS sequencing for qualitative information and real-time PCR for quantitative information, seeking to relate the mycobiomes with the content of caffeine and chlorogenic acid in leaves. <i>Dothideomycetes</i>, <i>Wallemiomycetes</i>, and <i>Tremellomycetes</i> are the dominant classes of fungi. The core leaf mycobiome among the three <i>Coffea</i> species is formed by <i>Hannaella</i>, <i>Cladosporium</i>, <i>Cryptococcus</i>, <i>Erythrobasidium,</i> and <i>Alternaria</i>. A network analysis showed that <i>Phoma</i>, an important <i>C. arabica</i> pathogen, is negatively related to six fungal species present in <i>C. racemosa</i> and <i>C. stenophylla</i> and absent in <i>C. arabica</i>. Finally, <i>C. arabica</i> have more than 35 times the concentration of caffeine and 2.5 times the concentration of chlorogenic acid than <i>C. stenophylla</i> and <i>C. racemosa</i>. The relationship between caffeine/chlorogenic acid content, the leaf mycobiome, and genotype pathogen resistance is discussed.
format	article
author	Leandro Pio de Sousa Oliveiro Guerreiro Filho Jorge Maurício Costa Mondego
author_facet	Leandro Pio de Sousa Oliveiro Guerreiro Filho Jorge Maurício Costa Mondego
author_sort	Leandro Pio de Sousa
title	Differences between the Leaf Mycobiome of <i>Coffea arabica</i> and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content
title_short	Differences between the Leaf Mycobiome of <i>Coffea arabica</i> and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content
title_full	Differences between the Leaf Mycobiome of <i>Coffea arabica</i> and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content
title_fullStr	Differences between the Leaf Mycobiome of <i>Coffea arabica</i> and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content
title_full_unstemmed	Differences between the Leaf Mycobiome of <i>Coffea arabica</i> and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content
title_sort	differences between the leaf mycobiome of <i>coffea arabica</i> and wild coffee species and their modulation by caffeine/chlorogenic acid content
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b17f091d3a794529842d47a9e76b0bb5
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Differences between the Leaf Mycobiome of <i>Coffea arabica</i> and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content

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