Vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse

Abstract Sow mortality attributable to pelvic organ prolapse (POP) has increased in the U.S. swine industry and continues to worsen. Two main objectives of this study were, (1) to develop a perineal scoring system that can be correlated with POP risk, and (2) identify POP risk-associated biological...

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Autores principales:	Zoë E. Kiefer, Lucas R. Koester, Lucas Showman, Jamie M. Studer, Amanda L. Chipman, Aileen F. Keating, Stephan Schmitz-Esser, Jason W. Ross
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b17f384238d34d99ab4a7d5b5553a3b7
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spelling	oai:doaj.org-article:b17f384238d34d99ab4a7d5b5553a3b72021-12-02T11:39:39ZVaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse10.1038/s41598-021-85367-32045-2322https://doaj.org/article/b17f384238d34d99ab4a7d5b5553a3b72021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85367-3https://doaj.org/toc/2045-2322Abstract Sow mortality attributable to pelvic organ prolapse (POP) has increased in the U.S. swine industry and continues to worsen. Two main objectives of this study were, (1) to develop a perineal scoring system that can be correlated with POP risk, and (2) identify POP risk-associated biological factors. To assess POP risk during late gestation, sows (n = 213) were scored using a newly developed perineal scoring (PS) system. Sows scored as PS1 (low), PS2 (moderate), or PS3 (high) based on POP risk. Subsequently, 1.5, 0.8, and 23.1% of sows scored PS1, PS2, or PS3, respectively, experienced POP. To identify biomarkers, serum and vaginal swabs were collected from late gestation sows differing in PS. Using GC–MS, 82 serum metabolite differences between PS1 and PS3 animals (P < 0.05) were identified. Vaginal swabs were utilized for 16S rRNA gene sequencing and differences in vaginal microbiomes between PS1 and PS3 animals were detected on a community level (P < 0.01) along with differences in abundances of 89 operational taxonomic units (P < 0.05). Collectively, these data demonstrate that sows with greater POP risk have differential serum metabolites and vaginal microflora. Additionally, an initial and novel characterization of the sow vaginal microbiome was determined.Zoë E. KieferLucas R. KoesterLucas ShowmanJamie M. StuderAmanda L. ChipmanAileen F. KeatingStephan Schmitz-EsserJason W. RossNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zoë E. Kiefer Lucas R. Koester Lucas Showman Jamie M. Studer Amanda L. Chipman Aileen F. Keating Stephan Schmitz-Esser Jason W. Ross Vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse
description	Abstract Sow mortality attributable to pelvic organ prolapse (POP) has increased in the U.S. swine industry and continues to worsen. Two main objectives of this study were, (1) to develop a perineal scoring system that can be correlated with POP risk, and (2) identify POP risk-associated biological factors. To assess POP risk during late gestation, sows (n = 213) were scored using a newly developed perineal scoring (PS) system. Sows scored as PS1 (low), PS2 (moderate), or PS3 (high) based on POP risk. Subsequently, 1.5, 0.8, and 23.1% of sows scored PS1, PS2, or PS3, respectively, experienced POP. To identify biomarkers, serum and vaginal swabs were collected from late gestation sows differing in PS. Using GC–MS, 82 serum metabolite differences between PS1 and PS3 animals (P < 0.05) were identified. Vaginal swabs were utilized for 16S rRNA gene sequencing and differences in vaginal microbiomes between PS1 and PS3 animals were detected on a community level (P < 0.01) along with differences in abundances of 89 operational taxonomic units (P < 0.05). Collectively, these data demonstrate that sows with greater POP risk have differential serum metabolites and vaginal microflora. Additionally, an initial and novel characterization of the sow vaginal microbiome was determined.
format	article
author	Zoë E. Kiefer Lucas R. Koester Lucas Showman Jamie M. Studer Amanda L. Chipman Aileen F. Keating Stephan Schmitz-Esser Jason W. Ross
author_facet	Zoë E. Kiefer Lucas R. Koester Lucas Showman Jamie M. Studer Amanda L. Chipman Aileen F. Keating Stephan Schmitz-Esser Jason W. Ross
author_sort	Zoë E. Kiefer
title	Vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse
title_short	Vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse
title_full	Vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse
title_fullStr	Vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse
title_full_unstemmed	Vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse
title_sort	vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b17f384238d34d99ab4a7d5b5553a3b7
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Vaginal microbiome and serum metabolite differences in late gestation commercial sows at risk for pelvic organ prolapse

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