Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats

Bahaa Al-Trad,1 Alaa Aljabali,2 Mazhar Al Zoubi,3 Malek Shehab,1 Sahar Omari11Department of Biological Sciences, Yarmouk University, Irbid, Jordan; 2Department of Pharmaceutical Sciences, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan; 3Department of Basic Medical Sciences, Faculty of Medici...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Al-Trad B, Aljabali A, Al Zoubi M, Shehab M, Omari S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Gold Nanoparticles Prostatic Hyperplasia Testosterone Rats Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b18e598c138241968de0166b73f512e0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b18e598c138241968de0166b73f512e0
record_format	dspace
spelling	oai:doaj.org-article:b18e598c138241968de0166b73f512e02021-12-02T05:40:27ZEffect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats1178-2013https://doaj.org/article/b18e598c138241968de0166b73f512e02019-05-01T00:00:00Zhttps://www.dovepress.com/effect-of-gold-nanoparticles-treatment-on-the-testosterone-induced-ben-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Bahaa Al-Trad,1 Alaa Aljabali,2 Mazhar Al Zoubi,3 Malek Shehab,1 Sahar Omari11Department of Biological Sciences, Yarmouk University, Irbid, Jordan; 2Department of Pharmaceutical Sciences, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan; 3Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, JordanBackground: Gold nanoparticles (AuNps) are promising agents for prostate cancer therapy. Herein, the in vivo effects of 20 and 50 nm sized AuNps on experimentally induced benign prostatic hyperplasia (BPH) was examined.Materials and methods: Adult male rats were divided into four groups (n=6–8 each). A negative control group and three groups were injected daily with testosterone (3 mg/kg/subcutaneously) to induce BPH. Animals receiving testosterone were randomized to untreated BPH group and two BPH groups which were treated intraperitoneally with 20 and 50 nm AuNps (5 mg/kg/daily) in addition to testosterone. After three weeks, histopathological changes and serum levels of testosterone and dihydrotestosterone (DHT) were analyzed. In addition, the prostate tissue levels of transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor-a (VEGF-A) and interleukin-6 (IL-6) were measured using ELISA.Results: There were significant increases in the prostate weight/body weight ratio, serum testosterone and DHT and in the prostate tissue content of TGF-β1, IL-6 and VEGF-A in the untreated BPH group. histological examination showed morphological abnormalities with more proliferation in the glandular epithelial and stromal area and with abundant epithelial papillary folds in the BPH group. Simultaneous administration of 50 nm AuNps with testosterone tended to increase the prostate weight/body weight ratio and increase the tissue level of IL-6 in compared to the BPH group. Conversely, treatment with 20 nm AuNps significantly reduced the elevated tissue content of TGF-β1, IL-6, and VEGF-A. Histopathological examination also showed that 20 nm but not the 50 nm AuNps administration ameliorates testosterone-induced prostatic hyperplasia.Conclusions: In experimentally induced BPH, AuNps can inhibit the progression of BPH in a size-dependent manner. while 20 nm AuNps ameliorate BPH by its inhibitory effects on the prostatic cell proliferation, inflammation and angiogenesis, the 50 nm AuNps could potentially exacerbate the development of BPH in rats, mainly through enhancing the inflammatory process.Keywords: gold nanoparticles, prostatic hyperplasia, testosterone, ratsAl-Trad BAljabali AAl Zoubi MShehab MOmari SDove Medical PressarticleGold NanoparticlesProstatic HyperplasiaTestosteroneRatsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3145-3154 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gold Nanoparticles Prostatic Hyperplasia Testosterone Rats Medicine (General) R5-920
spellingShingle	Gold Nanoparticles Prostatic Hyperplasia Testosterone Rats Medicine (General) R5-920 Al-Trad B Aljabali A Al Zoubi M Shehab M Omari S Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats
description	Bahaa Al-Trad,1 Alaa Aljabali,2 Mazhar Al Zoubi,3 Malek Shehab,1 Sahar Omari11Department of Biological Sciences, Yarmouk University, Irbid, Jordan; 2Department of Pharmaceutical Sciences, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan; 3Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, JordanBackground: Gold nanoparticles (AuNps) are promising agents for prostate cancer therapy. Herein, the in vivo effects of 20 and 50 nm sized AuNps on experimentally induced benign prostatic hyperplasia (BPH) was examined.Materials and methods: Adult male rats were divided into four groups (n=6–8 each). A negative control group and three groups were injected daily with testosterone (3 mg/kg/subcutaneously) to induce BPH. Animals receiving testosterone were randomized to untreated BPH group and two BPH groups which were treated intraperitoneally with 20 and 50 nm AuNps (5 mg/kg/daily) in addition to testosterone. After three weeks, histopathological changes and serum levels of testosterone and dihydrotestosterone (DHT) were analyzed. In addition, the prostate tissue levels of transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor-a (VEGF-A) and interleukin-6 (IL-6) were measured using ELISA.Results: There were significant increases in the prostate weight/body weight ratio, serum testosterone and DHT and in the prostate tissue content of TGF-β1, IL-6 and VEGF-A in the untreated BPH group. histological examination showed morphological abnormalities with more proliferation in the glandular epithelial and stromal area and with abundant epithelial papillary folds in the BPH group. Simultaneous administration of 50 nm AuNps with testosterone tended to increase the prostate weight/body weight ratio and increase the tissue level of IL-6 in compared to the BPH group. Conversely, treatment with 20 nm AuNps significantly reduced the elevated tissue content of TGF-β1, IL-6, and VEGF-A. Histopathological examination also showed that 20 nm but not the 50 nm AuNps administration ameliorates testosterone-induced prostatic hyperplasia.Conclusions: In experimentally induced BPH, AuNps can inhibit the progression of BPH in a size-dependent manner. while 20 nm AuNps ameliorate BPH by its inhibitory effects on the prostatic cell proliferation, inflammation and angiogenesis, the 50 nm AuNps could potentially exacerbate the development of BPH in rats, mainly through enhancing the inflammatory process.Keywords: gold nanoparticles, prostatic hyperplasia, testosterone, rats
format	article
author	Al-Trad B Aljabali A Al Zoubi M Shehab M Omari S
author_facet	Al-Trad B Aljabali A Al Zoubi M Shehab M Omari S
author_sort	Al-Trad B
title	Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats
title_short	Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats
title_full	Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats
title_fullStr	Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats
title_full_unstemmed	Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats
title_sort	effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/b18e598c138241968de0166b73f512e0
work_keys_str_mv	AT altradb effectofgoldnanoparticlestreatmentonthetestosteroneinducedbenignprostatichyperplasiainrats AT aljabalia effectofgoldnanoparticlestreatmentonthetestosteroneinducedbenignprostatichyperplasiainrats AT alzoubim effectofgoldnanoparticlestreatmentonthetestosteroneinducedbenignprostatichyperplasiainrats AT shehabm effectofgoldnanoparticlestreatmentonthetestosteroneinducedbenignprostatichyperplasiainrats AT omaris effectofgoldnanoparticlestreatmentonthetestosteroneinducedbenignprostatichyperplasiainrats
_version_	1718400316068593664

Effect of gold nanoparticles treatment on the testosterone-induced benign prostatic hyperplasia in rats

Ejemplares similares