Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron

Abstract The organogenesis and development of reproductive organs, i.e., stamen and gynoecium, are important floral characteristics that are closely related to pollinators and reproductive fitness. As a genus from Magnoliaceae, Liriodendron has only two relict species: L. chinense and L. tulipifera....

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Autores principales:	Huanhuan Liu, Lichun Yang, Zhonghua Tu, Shenghua Zhu, Chengge Zhang, Huogen Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b19425a5d69d4518940881ff3560a6ba
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spelling	oai:doaj.org-article:b19425a5d69d4518940881ff3560a6ba2021-12-02T16:36:03ZGenome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron10.1038/s41598-021-85927-72045-2322https://doaj.org/article/b19425a5d69d4518940881ff3560a6ba2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85927-7https://doaj.org/toc/2045-2322Abstract The organogenesis and development of reproductive organs, i.e., stamen and gynoecium, are important floral characteristics that are closely related to pollinators and reproductive fitness. As a genus from Magnoliaceae, Liriodendron has only two relict species: L. chinense and L. tulipifera. Despite the similar flower shapes of these species, their natural seed-setting rates differ significantly, implying interspecies difference in floral organogenesis and development. MADS-box genes, which participate in floral organogenesis and development, remain unexplored in Liriodendron. Here, to explore the interspecies difference in floral organogenesis and development and identify MADS-box genes in Liriodendron, we examined the stamen and gynoecium primordia of the two Liriodendron species by scanning electron microscopy combined with paraffin sectioning, and then collected two types of primordia for RNA-seq. A total of 12 libraries were constructed and 42,268 genes were identified, including 35,269 reference genes and 6,999 new genes. Monoterpenoid biosynthesis was enriched in L. tulipifera. Genome-wide analysis of 32 MADS-box genes was conducted, including phylogenetic trees, exon/intron structures, and conserved motif distributions. Twenty-six genes were anchored on 17 scaffolds, and six new genes had no location information. The expression profiles of MIKC-type genes via RT-qPCR acrossing six stamen and gynoecium developmental stages indicates that the PI-like, AG/STK-like, SEP-like, and SVP-like genes may contribute to the species-specific differentiation of the organogenesis and development of reproductive organs in Liriodendron. Our findings laid the groundwork for the future exploration of the mechanism underlying on the interspecific differences in reproductive organ development and fitness in Liriodendron.Huanhuan LiuLichun YangZhonghua TuShenghua ZhuChengge ZhangHuogen LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Huanhuan Liu Lichun Yang Zhonghua Tu Shenghua Zhu Chengge Zhang Huogen Li Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron
description	Abstract The organogenesis and development of reproductive organs, i.e., stamen and gynoecium, are important floral characteristics that are closely related to pollinators and reproductive fitness. As a genus from Magnoliaceae, Liriodendron has only two relict species: L. chinense and L. tulipifera. Despite the similar flower shapes of these species, their natural seed-setting rates differ significantly, implying interspecies difference in floral organogenesis and development. MADS-box genes, which participate in floral organogenesis and development, remain unexplored in Liriodendron. Here, to explore the interspecies difference in floral organogenesis and development and identify MADS-box genes in Liriodendron, we examined the stamen and gynoecium primordia of the two Liriodendron species by scanning electron microscopy combined with paraffin sectioning, and then collected two types of primordia for RNA-seq. A total of 12 libraries were constructed and 42,268 genes were identified, including 35,269 reference genes and 6,999 new genes. Monoterpenoid biosynthesis was enriched in L. tulipifera. Genome-wide analysis of 32 MADS-box genes was conducted, including phylogenetic trees, exon/intron structures, and conserved motif distributions. Twenty-six genes were anchored on 17 scaffolds, and six new genes had no location information. The expression profiles of MIKC-type genes via RT-qPCR acrossing six stamen and gynoecium developmental stages indicates that the PI-like, AG/STK-like, SEP-like, and SVP-like genes may contribute to the species-specific differentiation of the organogenesis and development of reproductive organs in Liriodendron. Our findings laid the groundwork for the future exploration of the mechanism underlying on the interspecific differences in reproductive organ development and fitness in Liriodendron.
format	article
author	Huanhuan Liu Lichun Yang Zhonghua Tu Shenghua Zhu Chengge Zhang Huogen Li
author_facet	Huanhuan Liu Lichun Yang Zhonghua Tu Shenghua Zhu Chengge Zhang Huogen Li
author_sort	Huanhuan Liu
title	Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron
title_short	Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron
title_full	Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron
title_fullStr	Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron
title_full_unstemmed	Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron
title_sort	genome-wide identification of mikc-type genes related to stamen and gynoecium development in liriodendron
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b19425a5d69d4518940881ff3560a6ba
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Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron

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