Renoprotective Effect of Formononetin by Suppressing Smad3 Expression in Db/Db Mice

Jiawei Lv,1 Kai Zhuang,1 Xiyu Jiang,1 Heqing Huang,1,2 Shijian Quan1 1Department of Pharmacology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China; 2Laboratory of Pharmacology & Toxicology, School of Pharmaceu...

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Autores principales:	Lv J, Zhuang K, Jiang X, Huang H, Quan S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	formononetin diabetic nephropathy db/db mice renal fibrosis expression of smad3 antioxidants Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/b1a10d2d3e5b4d22a80a21ddc8819032
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spelling	oai:doaj.org-article:b1a10d2d3e5b4d22a80a21ddc88190322021-12-02T10:06:15ZRenoprotective Effect of Formononetin by Suppressing Smad3 Expression in Db/Db Mice1178-7007https://doaj.org/article/b1a10d2d3e5b4d22a80a21ddc88190322020-09-01T00:00:00Zhttps://www.dovepress.com/renoprotective-effect-of-formononetin-by-suppressing-smad3-expression--peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Jiawei Lv,1 Kai Zhuang,1 Xiyu Jiang,1 Heqing Huang,1,2 Shijian Quan1 1Department of Pharmacology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China; 2Laboratory of Pharmacology & Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, People’s Republic of ChinaCorrespondence: Heqing Huang; Shijian QuanDepartment of Pharmacology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, No. 232 East Wai Huan Road, Guangzhou 510006, People’s Republic of ChinaTel +86 1 392 211 9719Email huangheq@mail.sysu.edu.cn; quansj@gzucm.edu.cnPurpose: Glomerular sclerosis and renal interstitial fibrosis are the most important pathologies in the development of kidney damage under diabetic conditions. Smad3 plays antagonistic roles in high glucose-induced renal tubular fibrosis, which is an important treatment target for diabetic nephropathy (DN). Formononetin (FMN) has multiple effects on diabetic vascular complications including DN. However, whether it plays an anti-fibrosis role by regulating smad3 is unclear. The purpose of this study was to evaluate the renoprotective effect of FMN by suppressing smad3 expression in db/db mice.Methods: FMN was orally administered to db/db mice with a dose of 25 or 50 mg/kg/day for 8 weeks. At the end of the study, serum, urine, and kidney samples were collected for biochemical and pathological examinations. The expressions of proteins and mRNA associated with renal fibrosis were determined by biochemical, histological, immunofluorescence, and real-time PCR analysis.Results: The results showed that FMN substantially improved the glucolipid metabolism, reduced the oxidative stress, and protected renal function in db/db mice. Meanwhile, protein and mRNA expression of smad3 and related regulatory factor of extracellular matrix deposition were significantly suppressed.Conclusion: The present study suggested that FMN has a good renoprotective effect in DN, which plays an anti-fibrosis role in db/db mice by suppressing the expression of smad3.Keywords: formononetin, diabetic nephropathy, db/db mice, renal fibrosis, expression of smad3, antioxidantsLv JZhuang KJiang XHuang HQuan SDove Medical Pressarticleformononetindiabetic nephropathydb/db micerenal fibrosisexpression of smad3antioxidantsSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 3313-3324 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	formononetin diabetic nephropathy db/db mice renal fibrosis expression of smad3 antioxidants Specialties of internal medicine RC581-951
spellingShingle	formononetin diabetic nephropathy db/db mice renal fibrosis expression of smad3 antioxidants Specialties of internal medicine RC581-951 Lv J Zhuang K Jiang X Huang H Quan S Renoprotective Effect of Formononetin by Suppressing Smad3 Expression in Db/Db Mice
description	Jiawei Lv,1 Kai Zhuang,1 Xiyu Jiang,1 Heqing Huang,1,2 Shijian Quan1 1Department of Pharmacology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, People’s Republic of China; 2Laboratory of Pharmacology & Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, People’s Republic of ChinaCorrespondence: Heqing Huang; Shijian QuanDepartment of Pharmacology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, No. 232 East Wai Huan Road, Guangzhou 510006, People’s Republic of ChinaTel +86 1 392 211 9719Email huangheq@mail.sysu.edu.cn; quansj@gzucm.edu.cnPurpose: Glomerular sclerosis and renal interstitial fibrosis are the most important pathologies in the development of kidney damage under diabetic conditions. Smad3 plays antagonistic roles in high glucose-induced renal tubular fibrosis, which is an important treatment target for diabetic nephropathy (DN). Formononetin (FMN) has multiple effects on diabetic vascular complications including DN. However, whether it plays an anti-fibrosis role by regulating smad3 is unclear. The purpose of this study was to evaluate the renoprotective effect of FMN by suppressing smad3 expression in db/db mice.Methods: FMN was orally administered to db/db mice with a dose of 25 or 50 mg/kg/day for 8 weeks. At the end of the study, serum, urine, and kidney samples were collected for biochemical and pathological examinations. The expressions of proteins and mRNA associated with renal fibrosis were determined by biochemical, histological, immunofluorescence, and real-time PCR analysis.Results: The results showed that FMN substantially improved the glucolipid metabolism, reduced the oxidative stress, and protected renal function in db/db mice. Meanwhile, protein and mRNA expression of smad3 and related regulatory factor of extracellular matrix deposition were significantly suppressed.Conclusion: The present study suggested that FMN has a good renoprotective effect in DN, which plays an anti-fibrosis role in db/db mice by suppressing the expression of smad3.Keywords: formononetin, diabetic nephropathy, db/db mice, renal fibrosis, expression of smad3, antioxidants
format	article
author	Lv J Zhuang K Jiang X Huang H Quan S
author_facet	Lv J Zhuang K Jiang X Huang H Quan S
author_sort	Lv J
title	Renoprotective Effect of Formononetin by Suppressing Smad3 Expression in Db/Db Mice
title_short	Renoprotective Effect of Formononetin by Suppressing Smad3 Expression in Db/Db Mice
title_full	Renoprotective Effect of Formononetin by Suppressing Smad3 Expression in Db/Db Mice
title_fullStr	Renoprotective Effect of Formononetin by Suppressing Smad3 Expression in Db/Db Mice
title_full_unstemmed	Renoprotective Effect of Formononetin by Suppressing Smad3 Expression in Db/Db Mice
title_sort	renoprotective effect of formononetin by suppressing smad3 expression in db/db mice
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/b1a10d2d3e5b4d22a80a21ddc8819032
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_version_	1718397649093132288

Renoprotective Effect of Formononetin by Suppressing Smad3 Expression in Db/Db Mice

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