Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration

Axolotls are uniquely able to resolve spinal cord injuries, but little is known about the mechanisms underlying spinal cord regeneration. We previously found that tail amputation leads to reactivation of a developmental-like program in spinal cord ependymal cells (Rodrigo Albors et al., 2015), chara...

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Autores principales:	Emanuel Cura Costa, Leo Otsuki, Aida Rodrigo Albors, Elly M Tanaka, Osvaldo Chara
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	axolotl spinal cord regeneration computational model cell proliferation FUCCI cell cycle Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b1a93a44e4b346c1b6fe4dbbf2189ca1
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spelling	oai:doaj.org-article:b1a93a44e4b346c1b6fe4dbbf2189ca12021-11-09T06:42:42ZSpatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration10.7554/eLife.556652050-084Xe55665https://doaj.org/article/b1a93a44e4b346c1b6fe4dbbf2189ca12021-05-01T00:00:00Zhttps://elifesciences.org/articles/55665https://doaj.org/toc/2050-084XAxolotls are uniquely able to resolve spinal cord injuries, but little is known about the mechanisms underlying spinal cord regeneration. We previously found that tail amputation leads to reactivation of a developmental-like program in spinal cord ependymal cells (Rodrigo Albors et al., 2015), characterized by a high-proliferation zone emerging 4 days post-amputation (Rost et al., 2016). What underlies this spatiotemporal pattern of cell proliferation, however, remained unknown. Here, we use modeling, tightly linked to experimental data, to demonstrate that this regenerative response is consistent with a signal that recruits ependymal cells during ~85 hours after amputation within ~830 μm of the injury. We adapted Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) technology to axolotls (AxFUCCI) to visualize cell cycles in vivo. AxFUCCI axolotls confirmed the predicted appearance time and size of the injury-induced recruitment zone and revealed cell cycle synchrony between ependymal cells. Our modeling and imaging move us closer to understanding bona fide spinal cord regeneration.Emanuel Cura CostaLeo OtsukiAida Rodrigo AlborsElly M TanakaOsvaldo CharaeLife Sciences Publications Ltdarticleaxolotlspinal cord regenerationcomputational modelcell proliferationFUCCIcell cycleMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	axolotl spinal cord regeneration computational model cell proliferation FUCCI cell cycle Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	axolotl spinal cord regeneration computational model cell proliferation FUCCI cell cycle Medicine R Science Q Biology (General) QH301-705.5 Emanuel Cura Costa Leo Otsuki Aida Rodrigo Albors Elly M Tanaka Osvaldo Chara Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration
description	Axolotls are uniquely able to resolve spinal cord injuries, but little is known about the mechanisms underlying spinal cord regeneration. We previously found that tail amputation leads to reactivation of a developmental-like program in spinal cord ependymal cells (Rodrigo Albors et al., 2015), characterized by a high-proliferation zone emerging 4 days post-amputation (Rost et al., 2016). What underlies this spatiotemporal pattern of cell proliferation, however, remained unknown. Here, we use modeling, tightly linked to experimental data, to demonstrate that this regenerative response is consistent with a signal that recruits ependymal cells during ~85 hours after amputation within ~830 μm of the injury. We adapted Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) technology to axolotls (AxFUCCI) to visualize cell cycles in vivo. AxFUCCI axolotls confirmed the predicted appearance time and size of the injury-induced recruitment zone and revealed cell cycle synchrony between ependymal cells. Our modeling and imaging move us closer to understanding bona fide spinal cord regeneration.
format	article
author	Emanuel Cura Costa Leo Otsuki Aida Rodrigo Albors Elly M Tanaka Osvaldo Chara
author_facet	Emanuel Cura Costa Leo Otsuki Aida Rodrigo Albors Elly M Tanaka Osvaldo Chara
author_sort	Emanuel Cura Costa
title	Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration
title_short	Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration
title_full	Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration
title_fullStr	Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration
title_full_unstemmed	Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration
title_sort	spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/b1a93a44e4b346c1b6fe4dbbf2189ca1
work_keys_str_mv	AT emanuelcuracosta spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration AT leootsuki spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration AT aidarodrigoalbors spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration AT ellymtanaka spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration AT osvaldochara spatiotemporalcontrolofcellcycleaccelerationduringaxolotlspinalcordregeneration
_version_	1718441235542179840

Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration

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