Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencing

Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencing

Abstract A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read and long-read (nanopore) sequencing together with a new computational pipeline (FLAMES) is developed to enable isoform discovery, splicing analysis, and mutation detection in single cells. We identify t...

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Main Authors: Luyi Tian, Jafar S. Jabbari, Rachel Thijssen, Quentin Gouil, Shanika L. Amarasinghe, Oliver Voogd, Hasaru Kariyawasam, Mei R. M. Du, Jakob Schuster, Changqing Wang, Shian Su, Xueyi Dong, Charity W. Law, Alexis Lucattini, Yair David Joseph Prawer, Coralina Collar-Fernández, Jin D. Chung, Timur Naim, Audrey Chan, Chi Hai Ly, Gordon S. Lynch, James G. Ryall, Casey J. A. Anttila, Hongke Peng, Mary Ann Anderson, Christoffer Flensburg, Ian Majewski, Andrew W. Roberts, David C. S. Huang, Michael B. Clark, Matthew E. Ritchie
Format: article
Language:EN
Published: BMC 2021
Subjects:
Single-cell gene expression
Long-read sequencing
Splicing
Single-cell multi-omics
Biology (General)
QH301-705.5
Genetics
QH426-470
Online Access:https://doaj.org/article/b1aa5d4188e746cca4e60723f7229ae1
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