Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection

Abstract Pseudomonas is a ubiquitous genus that also causes human, animal and plant diseases. Most studies have focused on clinical P. aeruginosa strains from humans, but they are scarce on animal strains. This study was aimed to determine the occurrence of Pseudomonas spp. among faecal samples of h...

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Autores principales:	Lidia Ruiz-Roldán, Beatriz Rojo-Bezares, María de Toro, María López, Paula Toledano, Carmen Lozano, Gabriela Chichón, Lydia Alvarez-Erviti, Carmen Torres, Yolanda Sáenz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1afdd08946c44028408a43bf5705c3f
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spelling	oai:doaj.org-article:b1afdd08946c44028408a43bf5705c3f2021-12-02T15:32:59ZAntimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection10.1038/s41598-020-68575-12045-2322https://doaj.org/article/b1afdd08946c44028408a43bf5705c3f2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68575-1https://doaj.org/toc/2045-2322Abstract Pseudomonas is a ubiquitous genus that also causes human, animal and plant diseases. Most studies have focused on clinical P. aeruginosa strains from humans, but they are scarce on animal strains. This study was aimed to determine the occurrence of Pseudomonas spp. among faecal samples of healthy animals, and to analyse their antimicrobial resistance, and pathogenicity. Among 704 animal faecal samples analysed, 133 Pseudomonas spp. isolates (23 species) were recovered from 46 samples (6.5%), and classified in 75 different PFGE patterns. Low antimicrobial resistance levels were found, being the highest to aztreonam (50.3%). Five sequence-types (ST1648, ST1711, ST2096, ST2194, ST2252), two serotypes (O:3, O:6), and three virulotypes (analysing 15 virulence and quorum-sensing genes) were observed among the 9 P. aeruginosa strains. Type-3-Secretion System genes were absent in the six O:3-serotype strains that additionally showed high cytotoxicity and produced higher biofilm biomass, phenazine pigments and motility than PAO1 control strain. In these six strains, the exlAB locus, and other virulence genotypes (e.g. RGP69 pathogenicity island) exclusive of PA7 outliers were detected by whole genome sequencing. This is the first description of the presence of the ExlA exolysin in P. aeruginosa from healthy animals, highlighting their pathological importance.Lidia Ruiz-RoldánBeatriz Rojo-BezaresMaría de ToroMaría LópezPaula ToledanoCarmen LozanoGabriela ChichónLydia Alvarez-ErvitiCarmen TorresYolanda SáenzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lidia Ruiz-Roldán Beatriz Rojo-Bezares María de Toro María López Paula Toledano Carmen Lozano Gabriela Chichón Lydia Alvarez-Erviti Carmen Torres Yolanda Sáenz Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection
description	Abstract Pseudomonas is a ubiquitous genus that also causes human, animal and plant diseases. Most studies have focused on clinical P. aeruginosa strains from humans, but they are scarce on animal strains. This study was aimed to determine the occurrence of Pseudomonas spp. among faecal samples of healthy animals, and to analyse their antimicrobial resistance, and pathogenicity. Among 704 animal faecal samples analysed, 133 Pseudomonas spp. isolates (23 species) were recovered from 46 samples (6.5%), and classified in 75 different PFGE patterns. Low antimicrobial resistance levels were found, being the highest to aztreonam (50.3%). Five sequence-types (ST1648, ST1711, ST2096, ST2194, ST2252), two serotypes (O:3, O:6), and three virulotypes (analysing 15 virulence and quorum-sensing genes) were observed among the 9 P. aeruginosa strains. Type-3-Secretion System genes were absent in the six O:3-serotype strains that additionally showed high cytotoxicity and produced higher biofilm biomass, phenazine pigments and motility than PAO1 control strain. In these six strains, the exlAB locus, and other virulence genotypes (e.g. RGP69 pathogenicity island) exclusive of PA7 outliers were detected by whole genome sequencing. This is the first description of the presence of the ExlA exolysin in P. aeruginosa from healthy animals, highlighting their pathological importance.
format	article
author	Lidia Ruiz-Roldán Beatriz Rojo-Bezares María de Toro María López Paula Toledano Carmen Lozano Gabriela Chichón Lydia Alvarez-Erviti Carmen Torres Yolanda Sáenz
author_facet	Lidia Ruiz-Roldán Beatriz Rojo-Bezares María de Toro María López Paula Toledano Carmen Lozano Gabriela Chichón Lydia Alvarez-Erviti Carmen Torres Yolanda Sáenz
author_sort	Lidia Ruiz-Roldán
title	Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection
title_short	Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection
title_full	Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection
title_fullStr	Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection
title_full_unstemmed	Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection
title_sort	antimicrobial resistance and virulence of pseudomonas spp. among healthy animals: concern about exolysin exla detection
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b1afdd08946c44028408a43bf5705c3f
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Antimicrobial resistance and virulence of Pseudomonas spp. among healthy animals: concern about exolysin ExlA detection

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