Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations

The rapid improvement of space technologies is leading to the continuous increase of space missions that will soon bring humans back to the Moon and, in the coming future, toward longer interplanetary missions such as the one to Mars. The idea of living in space is charming and fascinating; however,...

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Autores principales:	Marco Calvaruso, Carmelo Militello, Luigi Minafra, Veronica La Regina, Filippo Torrisi, Gaia Pucci, Francesco P. Cammarata, Valentina Bravatà, Giusi I. Forte, Giorgio Russo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	space biology cancer biology TNBC simulated microgravity random positioning machine Science Q
Acceso en línea:	https://doaj.org/article/b1b8050a86e04eec8c4dc889fe65d7c5
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id	oai:doaj.org-article:b1b8050a86e04eec8c4dc889fe65d7c5
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spelling	oai:doaj.org-article:b1b8050a86e04eec8c4dc889fe65d7c52021-11-25T18:11:03ZBiological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations10.3390/life111111902075-1729https://doaj.org/article/b1b8050a86e04eec8c4dc889fe65d7c52021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1190https://doaj.org/toc/2075-1729The rapid improvement of space technologies is leading to the continuous increase of space missions that will soon bring humans back to the Moon and, in the coming future, toward longer interplanetary missions such as the one to Mars. The idea of living in space is charming and fascinating; however, the space environment is a harsh place to host human life and exposes the crew to many physical challenges. The absence of gravity experienced in space affects many aspects of human biology and can be reproduced in vitro with the help of microgravity simulators. Simulated microgravity (s-μg) is applied in many fields of research, ranging from cell biology to physics, including cancer biology. In our study, we aimed to characterize, at the biological and mechanical level, a Random Positioning Machine in order to simulate microgravity in an in vitro model of Triple-Negative Breast Cancer (TNBC). We investigated the effects played by s-μg by analyzing the change of expression of some genes that drive proliferation, survival, cell death, cancer stemness, and metastasis in the human MDA-MB-231 cell line. Besides the mechanical verification of the RPM used in our studies, our biological findings highlighted the impact of s-μg and its putative involvement in cancer progression.Marco CalvarusoCarmelo MilitelloLuigi MinafraVeronica La ReginaFilippo TorrisiGaia PucciFrancesco P. CammarataValentina BravatàGiusi I. ForteGiorgio RussoMDPI AGarticlespace biologycancer biologyTNBCsimulated microgravityrandom positioning machineScienceQENLife, Vol 11, Iss 1190, p 1190 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	space biology cancer biology TNBC simulated microgravity random positioning machine Science Q
spellingShingle	space biology cancer biology TNBC simulated microgravity random positioning machine Science Q Marco Calvaruso Carmelo Militello Luigi Minafra Veronica La Regina Filippo Torrisi Gaia Pucci Francesco P. Cammarata Valentina Bravatà Giusi I. Forte Giorgio Russo Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations
description	The rapid improvement of space technologies is leading to the continuous increase of space missions that will soon bring humans back to the Moon and, in the coming future, toward longer interplanetary missions such as the one to Mars. The idea of living in space is charming and fascinating; however, the space environment is a harsh place to host human life and exposes the crew to many physical challenges. The absence of gravity experienced in space affects many aspects of human biology and can be reproduced in vitro with the help of microgravity simulators. Simulated microgravity (s-μg) is applied in many fields of research, ranging from cell biology to physics, including cancer biology. In our study, we aimed to characterize, at the biological and mechanical level, a Random Positioning Machine in order to simulate microgravity in an in vitro model of Triple-Negative Breast Cancer (TNBC). We investigated the effects played by s-μg by analyzing the change of expression of some genes that drive proliferation, survival, cell death, cancer stemness, and metastasis in the human MDA-MB-231 cell line. Besides the mechanical verification of the RPM used in our studies, our biological findings highlighted the impact of s-μg and its putative involvement in cancer progression.
format	article
author	Marco Calvaruso Carmelo Militello Luigi Minafra Veronica La Regina Filippo Torrisi Gaia Pucci Francesco P. Cammarata Valentina Bravatà Giusi I. Forte Giorgio Russo
author_facet	Marco Calvaruso Carmelo Militello Luigi Minafra Veronica La Regina Filippo Torrisi Gaia Pucci Francesco P. Cammarata Valentina Bravatà Giusi I. Forte Giorgio Russo
author_sort	Marco Calvaruso
title	Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations
title_short	Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations
title_full	Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations
title_fullStr	Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations
title_full_unstemmed	Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations
title_sort	biological and mechanical characterization of the random positioning machine (rpm) for microgravity simulations
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b1b8050a86e04eec8c4dc889fe65d7c5
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Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations

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