CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
Abstract Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in ba...
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Main Authors: | Katherine S. Wetzel, Carlos A. Guerrero-Bustamante, Rebekah M. Dedrick, Ching-Chung Ko, Krista G. Freeman, Haley G. Aull, Ashley M. Divens, Jeremy M. Rock, Kira M. Zack, Graham F. Hatfull |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2021
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Online Access: | https://doaj.org/article/b1b89e6a2de841798e39f54d739bc4c6 |
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