Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai

Abstract Human adenovirus F (HAdV-F) is one of the major causative species detected in acute gastroenteritis in children worldwide. HAdV-F is composed of serotypes 40 and 41. Most studies have reported the prevalence of HAdV-41 and focused on its epidemiologic characteristics. In this study, sevente...

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Autores principales:	Peng Li, Lang Yang, Jiayin Guo, Wenwei Zou, Xuebin Xu, Xiaoxia Yang, Xinying Du, Shaofu Qiu, Hongbin Song
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1bb7c540cca410c8292da3398f491d4
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spelling	oai:doaj.org-article:b1bb7c540cca410c8292da3398f491d42021-12-02T16:06:02ZCirculation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai10.1038/s41598-017-01293-32045-2322https://doaj.org/article/b1bb7c540cca410c8292da3398f491d42017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01293-3https://doaj.org/toc/2045-2322Abstract Human adenovirus F (HAdV-F) is one of the major causative species detected in acute gastroenteritis in children worldwide. HAdV-F is composed of serotypes 40 and 41. Most studies have reported the prevalence of HAdV-41 and focused on its epidemiologic characteristics. In this study, seventeen samples were identified as HAdV-41 out of 273 fecal specimens from children with acute diarrhea in Shanghai. Five isolates were isolated and subjected to whole genome sequencing and analysis to characterize the genetic variation and evolution. Full genome analysis revealed low genetic variation (99.07–99.92% identity) among the isolates, and InDels are observed in the E2A gene and the hexon gene compared to the reference strain NIVD103. Phylogenetic analysis showed that all isolates mainly formed two genome-type clusters but with incongruence in the trees of whole genomes and individual genes. The recombination breakpoints of the five isolates were inferred by the Recombination Detection Program (RDP) and varied in the number and location of the recombination events, indicating different evolution origins. Overall, our study highlights the genetic diversity of HAdV-41 isolates circulating in Shanghai, which may have evolved from inter-strain recombination.Peng LiLang YangJiayin GuoWenwei ZouXuebin XuXiaoxia YangXinying DuShaofu QiuHongbin SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Li Lang Yang Jiayin Guo Wenwei Zou Xuebin Xu Xiaoxia Yang Xinying Du Shaofu Qiu Hongbin Song Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai
description	Abstract Human adenovirus F (HAdV-F) is one of the major causative species detected in acute gastroenteritis in children worldwide. HAdV-F is composed of serotypes 40 and 41. Most studies have reported the prevalence of HAdV-41 and focused on its epidemiologic characteristics. In this study, seventeen samples were identified as HAdV-41 out of 273 fecal specimens from children with acute diarrhea in Shanghai. Five isolates were isolated and subjected to whole genome sequencing and analysis to characterize the genetic variation and evolution. Full genome analysis revealed low genetic variation (99.07–99.92% identity) among the isolates, and InDels are observed in the E2A gene and the hexon gene compared to the reference strain NIVD103. Phylogenetic analysis showed that all isolates mainly formed two genome-type clusters but with incongruence in the trees of whole genomes and individual genes. The recombination breakpoints of the five isolates were inferred by the Recombination Detection Program (RDP) and varied in the number and location of the recombination events, indicating different evolution origins. Overall, our study highlights the genetic diversity of HAdV-41 isolates circulating in Shanghai, which may have evolved from inter-strain recombination.
format	article
author	Peng Li Lang Yang Jiayin Guo Wenwei Zou Xuebin Xu Xiaoxia Yang Xinying Du Shaofu Qiu Hongbin Song
author_facet	Peng Li Lang Yang Jiayin Guo Wenwei Zou Xuebin Xu Xiaoxia Yang Xinying Du Shaofu Qiu Hongbin Song
author_sort	Peng Li
title	Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai
title_short	Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai
title_full	Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai
title_fullStr	Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai
title_full_unstemmed	Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai
title_sort	circulation of hadv-41 with diverse genome types and recombination in acute gastroenteritis among children in shanghai
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b1bb7c540cca410c8292da3398f491d4
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Circulation of HAdV-41 with diverse genome types and recombination in acute gastroenteritis among children in Shanghai

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