Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis

Abstract Exosomes are small membrane particles which are widely found in various cell lines and physiological fluids in mammalian. MicroRNAs (miRNAs) enclosed in exosomes have been identified as proper signatures for many diseases and response to therapies. However, the composition of exosomes and e...

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Autores principales:	Zhanpeng Sun, Tong Hao, Jinze Tian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1be758b6bfe42689ced79b305756206
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spelling	oai:doaj.org-article:b1be758b6bfe42689ced79b3057562062021-12-02T16:06:57ZIdentification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis10.1038/s41598-017-00884-42045-2322https://doaj.org/article/b1be758b6bfe42689ced79b3057562062017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00884-4https://doaj.org/toc/2045-2322Abstract Exosomes are small membrane particles which are widely found in various cell lines and physiological fluids in mammalian. MicroRNAs (miRNAs) enclosed in exosomes have been identified as proper signatures for many diseases and response to therapies. However, the composition of exosomes and enclosed miRNAs in fishes has not been investigated. Cynoglossus semilaevis is an important commercial flatfish with ambiguous distinction between males and females before sex maturation, which leads to screening difficulty in reproduction and cultivation. An effective detection method was required for sex differentiation of C. semilaevis. In this work, we successfully identified exosomes in C. semilaevis serum. The analysis of nucleotide composition showed that miRNA dominated in exosomes. Thereafter the miRNA profiles in exosomes from males and females were sequenced and compared to identify the signature miRNAs corresponding to sex differentiation. The functions of signature miRNAs were analyzed by target matching and annotation. Furthermore, 7 miRNAs with high expression in males were selected from signature miRNAs as the markers for sex identification with their expression profiles verified by real time quantitative PCR. Exosomes were first found in fish serum in this work. Investigation of marker miRNAs supplies an effective index for the filtration of male and female C. semilaevis in cultivation.Zhanpeng SunTong HaoJinze TianNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhanpeng Sun Tong Hao Jinze Tian Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis
description	Abstract Exosomes are small membrane particles which are widely found in various cell lines and physiological fluids in mammalian. MicroRNAs (miRNAs) enclosed in exosomes have been identified as proper signatures for many diseases and response to therapies. However, the composition of exosomes and enclosed miRNAs in fishes has not been investigated. Cynoglossus semilaevis is an important commercial flatfish with ambiguous distinction between males and females before sex maturation, which leads to screening difficulty in reproduction and cultivation. An effective detection method was required for sex differentiation of C. semilaevis. In this work, we successfully identified exosomes in C. semilaevis serum. The analysis of nucleotide composition showed that miRNA dominated in exosomes. Thereafter the miRNA profiles in exosomes from males and females were sequenced and compared to identify the signature miRNAs corresponding to sex differentiation. The functions of signature miRNAs were analyzed by target matching and annotation. Furthermore, 7 miRNAs with high expression in males were selected from signature miRNAs as the markers for sex identification with their expression profiles verified by real time quantitative PCR. Exosomes were first found in fish serum in this work. Investigation of marker miRNAs supplies an effective index for the filtration of male and female C. semilaevis in cultivation.
format	article
author	Zhanpeng Sun Tong Hao Jinze Tian
author_facet	Zhanpeng Sun Tong Hao Jinze Tian
author_sort	Zhanpeng Sun
title	Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis
title_short	Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis
title_full	Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis
title_fullStr	Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis
title_full_unstemmed	Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis
title_sort	identification of exosomes and its signature mirnas of male and female cynoglossus semilaevis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b1be758b6bfe42689ced79b305756206
work_keys_str_mv	AT zhanpengsun identificationofexosomesanditssignaturemirnasofmaleandfemalecynoglossussemilaevis AT tonghao identificationofexosomesanditssignaturemirnasofmaleandfemalecynoglossussemilaevis AT jinzetian identificationofexosomesanditssignaturemirnasofmaleandfemalecynoglossussemilaevis
_version_	1718384799691833344

Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis

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