Using Bioinformatics to Develop and Test Hypotheses: <italic toggle="yes">E. coli</italic>-Specific Virulence Determinants

Bioinformatics, the use of computer resources to understand biological information, is an important tool in research, and can be easily integrated into the curriculum of undergraduate courses. Such an example is provided in this series of four activities that introduces students to the field of bioi...

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Autores principales:	Joanna R. Klein, Theresa Gulsvig
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2012
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b1c38aea02774f2d82ca26822dbd0fd7
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spelling	oai:doaj.org-article:b1c38aea02774f2d82ca26822dbd0fd72021-11-15T15:03:41ZUsing Bioinformatics to Develop and Test Hypotheses: <italic toggle="yes">E. coli</italic>-Specific Virulence Determinants10.1128/jmbe.v13i2.4511935-78851935-7877https://doaj.org/article/b1c38aea02774f2d82ca26822dbd0fd72012-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v13i2.451https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Bioinformatics, the use of computer resources to understand biological information, is an important tool in research, and can be easily integrated into the curriculum of undergraduate courses. Such an example is provided in this series of four activities that introduces students to the field of bioinformatics as they design PCR based tests for pathogenic E. coli strains. A variety of computer tools are used including BLAST searches at NCBI, bacterial genome searches at the Integrated Microbial Genomes (IMG) database, protein analysis at Pfam and literature research at PubMed. In the process, students also learn about virulence factors, enzyme function and horizontal gene transfer. Some or all of the four activities can be incorporated into microbiology or general biology courses taken by students at a variety of levels, ranging from high school through college. The activities build on one another as they teach and reinforce knowledge and skills, promote critical thinking, and provide for student collaboration and presentation. The computer-based activities can be done either in class or outside of class, thus are appropriate for inclusion in online or blended learning formats. Assessment data showed that students learned general microbiology concepts related to pathogenesis and enzyme function, gained skills in using tools of bioinformatics and molecular biology, and successfully developed and tested a scientific hypothesis.Joanna R. KleinTheresa GulsvigAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 13, Iss 2, Pp 161-169 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Joanna R. Klein Theresa Gulsvig Using Bioinformatics to Develop and Test Hypotheses: <italic toggle="yes">E. coli</italic>-Specific Virulence Determinants
description	Bioinformatics, the use of computer resources to understand biological information, is an important tool in research, and can be easily integrated into the curriculum of undergraduate courses. Such an example is provided in this series of four activities that introduces students to the field of bioinformatics as they design PCR based tests for pathogenic E. coli strains. A variety of computer tools are used including BLAST searches at NCBI, bacterial genome searches at the Integrated Microbial Genomes (IMG) database, protein analysis at Pfam and literature research at PubMed. In the process, students also learn about virulence factors, enzyme function and horizontal gene transfer. Some or all of the four activities can be incorporated into microbiology or general biology courses taken by students at a variety of levels, ranging from high school through college. The activities build on one another as they teach and reinforce knowledge and skills, promote critical thinking, and provide for student collaboration and presentation. The computer-based activities can be done either in class or outside of class, thus are appropriate for inclusion in online or blended learning formats. Assessment data showed that students learned general microbiology concepts related to pathogenesis and enzyme function, gained skills in using tools of bioinformatics and molecular biology, and successfully developed and tested a scientific hypothesis.
format	article
author	Joanna R. Klein Theresa Gulsvig
author_facet	Joanna R. Klein Theresa Gulsvig
author_sort	Joanna R. Klein
title	Using Bioinformatics to Develop and Test Hypotheses: <italic toggle="yes">E. coli</italic>-Specific Virulence Determinants
title_short	Using Bioinformatics to Develop and Test Hypotheses: <italic toggle="yes">E. coli</italic>-Specific Virulence Determinants
title_full	Using Bioinformatics to Develop and Test Hypotheses: <italic toggle="yes">E. coli</italic>-Specific Virulence Determinants
title_fullStr	Using Bioinformatics to Develop and Test Hypotheses: <italic toggle="yes">E. coli</italic>-Specific Virulence Determinants
title_full_unstemmed	Using Bioinformatics to Develop and Test Hypotheses: <italic toggle="yes">E. coli</italic>-Specific Virulence Determinants
title_sort	using bioinformatics to develop and test hypotheses: <italic toggle="yes">e. coli</italic>-specific virulence determinants
publisher	American Society for Microbiology
publishDate	2012
url	https://doaj.org/article/b1c38aea02774f2d82ca26822dbd0fd7
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Using Bioinformatics to Develop and Test Hypotheses: <italic toggle="yes">E. coli</italic>-Specific Virulence Determinants

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