Evaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of <i>Crotalus durissus terrificus</i> (South American Rattlesnake) Venom

Systemic envenomation by <i>Crotalus durissus terrificus</i> (South American rattlesnake) can cause coagulopathy, rabdomyolysis, acute kidney injury, and peripheral neuromuscular blockade, the latter resulting in flaccid paralysis. Previous studies have shown that plant products such as...

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Autores principales:	Isadora Caruso Fontana Oliveira, Edson Hideaki Yoshida, Murilo Melo Juste Dini, Ana Beatriz Olívio Paschoal, José Carlos Cogo, Maria Alice da Cruz-Höfling, Stephen Hyslop, Yoko Oshima-Franco
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antivenom phytochemical rattlesnake venom tannic acid Medicine R
Acceso en línea:	https://doaj.org/article/b1c52ffe5bdd490dadddc2e701a23964
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spelling	oai:doaj.org-article:b1c52ffe5bdd490dadddc2e701a239642021-11-25T19:08:56ZEvaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of <i>Crotalus durissus terrificus</i> (South American Rattlesnake) Venom10.3390/toxins131108012072-6651https://doaj.org/article/b1c52ffe5bdd490dadddc2e701a239642021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/801https://doaj.org/toc/2072-6651Systemic envenomation by <i>Crotalus durissus terrificus</i> (South American rattlesnake) can cause coagulopathy, rabdomyolysis, acute kidney injury, and peripheral neuromuscular blockade, the latter resulting in flaccid paralysis. Previous studies have shown that plant products such as tannic acid and theaflavin can protect against the neuromuscular blockade caused by <i>C. d. terrificus</i> venom in vitro. In this work, we used mouse-isolated phrenic nerve-diaphragm preparations to examine the ability of caffeic acid, chlorogenic acid, and quercetin to protect against <i>C. d. terrificus</i> venom-induced neuromuscular blockade in vitro. In addition, the ability of tannic acid to protect against the systemic effects of severe envenomation was assessed in rats. Preincubation of venom with caffeic acid (0.5 mg/mL), chlorogenic acid (1 mg/mL), or quercetin (0.5 mg/mL) failed to protect against venom (10 μg/mL)-induced neuromuscular blockade. In rats, venom (6 mg kg<sup>−1</sup>, i.p.) caused death in ~8 h, which was prevented by preincubation of venom with tannic acid or the administration of antivenom 2 h post-venom, whereas tannic acid given 2 h post-venom prolonged survival (~18.5 h) but did not prevent death. Tannic acid (in preincubation protocols or given 2 h post-venom) had a variable effect on blood creatinine and urea and blood/urine protein levels and prevented venom-induced leukocytosis. Tannic acid attenuated the histological lesions associated with renal damage in a manner similar to antivenom. The protective effect of tannic acid appeared to be mediated by interaction with venom proteins, as assessed by SDS-PAGE. These findings suggest that tannic acid could be a potentially useful ancillary treatment for envenomation by <i>C. d. terrificus</i>.Isadora Caruso Fontana OliveiraEdson Hideaki YoshidaMurilo Melo Juste DiniAna Beatriz Olívio PaschoalJosé Carlos CogoMaria Alice da Cruz-HöflingStephen HyslopYoko Oshima-FrancoMDPI AGarticleantivenomphytochemicalrattlesnake venomtannic acidMedicineRENToxins, Vol 13, Iss 801, p 801 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antivenom phytochemical rattlesnake venom tannic acid Medicine R
spellingShingle	antivenom phytochemical rattlesnake venom tannic acid Medicine R Isadora Caruso Fontana Oliveira Edson Hideaki Yoshida Murilo Melo Juste Dini Ana Beatriz Olívio Paschoal José Carlos Cogo Maria Alice da Cruz-Höfling Stephen Hyslop Yoko Oshima-Franco Evaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of <i>Crotalus durissus terrificus</i> (South American Rattlesnake) Venom
description	Systemic envenomation by <i>Crotalus durissus terrificus</i> (South American rattlesnake) can cause coagulopathy, rabdomyolysis, acute kidney injury, and peripheral neuromuscular blockade, the latter resulting in flaccid paralysis. Previous studies have shown that plant products such as tannic acid and theaflavin can protect against the neuromuscular blockade caused by <i>C. d. terrificus</i> venom in vitro. In this work, we used mouse-isolated phrenic nerve-diaphragm preparations to examine the ability of caffeic acid, chlorogenic acid, and quercetin to protect against <i>C. d. terrificus</i> venom-induced neuromuscular blockade in vitro. In addition, the ability of tannic acid to protect against the systemic effects of severe envenomation was assessed in rats. Preincubation of venom with caffeic acid (0.5 mg/mL), chlorogenic acid (1 mg/mL), or quercetin (0.5 mg/mL) failed to protect against venom (10 μg/mL)-induced neuromuscular blockade. In rats, venom (6 mg kg<sup>−1</sup>, i.p.) caused death in ~8 h, which was prevented by preincubation of venom with tannic acid or the administration of antivenom 2 h post-venom, whereas tannic acid given 2 h post-venom prolonged survival (~18.5 h) but did not prevent death. Tannic acid (in preincubation protocols or given 2 h post-venom) had a variable effect on blood creatinine and urea and blood/urine protein levels and prevented venom-induced leukocytosis. Tannic acid attenuated the histological lesions associated with renal damage in a manner similar to antivenom. The protective effect of tannic acid appeared to be mediated by interaction with venom proteins, as assessed by SDS-PAGE. These findings suggest that tannic acid could be a potentially useful ancillary treatment for envenomation by <i>C. d. terrificus</i>.
format	article
author	Isadora Caruso Fontana Oliveira Edson Hideaki Yoshida Murilo Melo Juste Dini Ana Beatriz Olívio Paschoal José Carlos Cogo Maria Alice da Cruz-Höfling Stephen Hyslop Yoko Oshima-Franco
author_facet	Isadora Caruso Fontana Oliveira Edson Hideaki Yoshida Murilo Melo Juste Dini Ana Beatriz Olívio Paschoal José Carlos Cogo Maria Alice da Cruz-Höfling Stephen Hyslop Yoko Oshima-Franco
author_sort	Isadora Caruso Fontana Oliveira
title	Evaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of <i>Crotalus durissus terrificus</i> (South American Rattlesnake) Venom
title_short	Evaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of <i>Crotalus durissus terrificus</i> (South American Rattlesnake) Venom
title_full	Evaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of <i>Crotalus durissus terrificus</i> (South American Rattlesnake) Venom
title_fullStr	Evaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of <i>Crotalus durissus terrificus</i> (South American Rattlesnake) Venom
title_full_unstemmed	Evaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of <i>Crotalus durissus terrificus</i> (South American Rattlesnake) Venom
title_sort	evaluation of protection by caffeic acid, chlorogenic acid, quercetin and tannic acid against the in vitro neurotoxicity and in vivo lethality of <i>crotalus durissus terrificus</i> (south american rattlesnake) venom
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/b1c52ffe5bdd490dadddc2e701a23964
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Evaluation of Protection by Caffeic Acid, Chlorogenic Acid, Quercetin and Tannic Acid against the In Vitro Neurotoxicity and In Vivo Lethality of <i>Crotalus durissus terrificus</i> (South American Rattlesnake) Venom

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