Tissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck

Ducks are the natural host and reservoir of influenza A virus (IAV), and as such are permissive to viral replication while being unharmed by most strains. It is not known which mechanisms of viral control are globally regulated during infection, and which are specific to tissues during infection. He...

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Autores principales:	Lee K. Campbell, Ximena Fleming-Canepa, Robert G. Webster, Katharine E. Magor
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	RNA-seq highly pathogenic avian influenza proinflammatory cytokines reservoir host duck (Anas platrhynchos) Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/b1c69aa4e4b543909f7d228eec406410
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spelling	oai:doaj.org-article:b1c69aa4e4b543909f7d228eec4064102021-11-05T11:48:30ZTissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck1664-322410.3389/fimmu.2021.786205https://doaj.org/article/b1c69aa4e4b543909f7d228eec4064102021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.786205/fullhttps://doaj.org/toc/1664-3224Ducks are the natural host and reservoir of influenza A virus (IAV), and as such are permissive to viral replication while being unharmed by most strains. It is not known which mechanisms of viral control are globally regulated during infection, and which are specific to tissues during infection. Here we compare transcript expression from tissues from Pekin ducks infected with a recombinant H5N1 strain A/Vietnam 1203/04 (VN1203) or an H5N2 strain A/British Columbia 500/05 using RNA-sequencing analysis and aligning reads to the NCBI assembly ZJU1.0 of the domestic duck (Anas platyrhynchos) genome. Highly pathogenic VN1203 replicated in lungs and showed systemic dissemination, while BC500, like most low pathogenic strains, replicated in the intestines. VN1203 infection induced robust differential expression of genes all three days post infection, while BC500 induced the greatest number of differentially expressed genes on day 2 post infection. While there were many genes globally upregulated in response to either VN1203 or BC500, tissue specific gene expression differences were observed. Lungs of ducks infected with VN1203 and intestines of birds infected with BC500, tissues important in influenza replication, showed highest upregulation of pattern recognition receptors and interferon stimulated genes early in the response. These tissues also appear to have specific downregulation of inflammatory components, with downregulation of distinct sets of proinflammatory cytokines in lung, and downregulation of key components of leukocyte recruitment and complement pathways in intestine. Our results suggest that global and tissue specific regulation patterns help the duck control viral replication as well as limit some inflammatory responses in tissues involved in replication to avoid damage.Lee K. CampbellLee K. CampbellXimena Fleming-CanepaRobert G. WebsterKatharine E. MagorKatharine E. MagorFrontiers Media S.A.articleRNA-seqhighly pathogenic avian influenzaproinflammatory cytokinesreservoir hostduck (Anas platrhynchos)Immunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA-seq highly pathogenic avian influenza proinflammatory cytokines reservoir host duck (Anas platrhynchos) Immunologic diseases. Allergy RC581-607
spellingShingle	RNA-seq highly pathogenic avian influenza proinflammatory cytokines reservoir host duck (Anas platrhynchos) Immunologic diseases. Allergy RC581-607 Lee K. Campbell Lee K. Campbell Ximena Fleming-Canepa Robert G. Webster Katharine E. Magor Katharine E. Magor Tissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck
description	Ducks are the natural host and reservoir of influenza A virus (IAV), and as such are permissive to viral replication while being unharmed by most strains. It is not known which mechanisms of viral control are globally regulated during infection, and which are specific to tissues during infection. Here we compare transcript expression from tissues from Pekin ducks infected with a recombinant H5N1 strain A/Vietnam 1203/04 (VN1203) or an H5N2 strain A/British Columbia 500/05 using RNA-sequencing analysis and aligning reads to the NCBI assembly ZJU1.0 of the domestic duck (Anas platyrhynchos) genome. Highly pathogenic VN1203 replicated in lungs and showed systemic dissemination, while BC500, like most low pathogenic strains, replicated in the intestines. VN1203 infection induced robust differential expression of genes all three days post infection, while BC500 induced the greatest number of differentially expressed genes on day 2 post infection. While there were many genes globally upregulated in response to either VN1203 or BC500, tissue specific gene expression differences were observed. Lungs of ducks infected with VN1203 and intestines of birds infected with BC500, tissues important in influenza replication, showed highest upregulation of pattern recognition receptors and interferon stimulated genes early in the response. These tissues also appear to have specific downregulation of inflammatory components, with downregulation of distinct sets of proinflammatory cytokines in lung, and downregulation of key components of leukocyte recruitment and complement pathways in intestine. Our results suggest that global and tissue specific regulation patterns help the duck control viral replication as well as limit some inflammatory responses in tissues involved in replication to avoid damage.
format	article
author	Lee K. Campbell Lee K. Campbell Ximena Fleming-Canepa Robert G. Webster Katharine E. Magor Katharine E. Magor
author_facet	Lee K. Campbell Lee K. Campbell Ximena Fleming-Canepa Robert G. Webster Katharine E. Magor Katharine E. Magor
author_sort	Lee K. Campbell
title	Tissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck
title_short	Tissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck
title_full	Tissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck
title_fullStr	Tissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck
title_full_unstemmed	Tissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck
title_sort	tissue specific transcriptome changes upon influenza a virus replication in the duck
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b1c69aa4e4b543909f7d228eec406410
work_keys_str_mv	AT leekcampbell tissuespecifictranscriptomechangesuponinfluenzaavirusreplicationintheduck AT leekcampbell tissuespecifictranscriptomechangesuponinfluenzaavirusreplicationintheduck AT ximenaflemingcanepa tissuespecifictranscriptomechangesuponinfluenzaavirusreplicationintheduck AT robertgwebster tissuespecifictranscriptomechangesuponinfluenzaavirusreplicationintheduck AT katharineemagor tissuespecifictranscriptomechangesuponinfluenzaavirusreplicationintheduck AT katharineemagor tissuespecifictranscriptomechangesuponinfluenzaavirusreplicationintheduck
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Tissue Specific Transcriptome Changes Upon Influenza A Virus Replication in the Duck

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