СARBAPENEMASE-PRODUCING GRAM-NEGATIVE BACTERIA IN A SPECIALIZED HOSPITAL OF ST. PETERSBURG

Species composition, susceptibility to antimicrobial agents, incidence and type of carbapenemase of carbapenemase-producing gram-negative bacteria, isolated in the Russian Research Centre of Radiology and Surgical Technologies from February 2014 to April 2016 were described. Screening of carbapenem-...

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Autores principales:	A. G. Polischouk, E. I. Jakubovich, O. V. Poluhina, V. V. Osovskich, V. I. Evtushenko
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2017
Materias:	carbapenemases bacterial sensitivity gram-negative bacteria maldi-tof ms species identification nosocomial infections Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/b1caa81b8f894021bda3cd87966d70f3
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spelling	oai:doaj.org-article:b1caa81b8f894021bda3cd87966d70f32021-11-22T07:09:48ZСARBAPENEMASE-PRODUCING GRAM-NEGATIVE BACTERIA IN A SPECIALIZED HOSPITAL OF ST. PETERSBURG2220-76192313-739810.15789/2220-7619-2017-2-181-192https://doaj.org/article/b1caa81b8f894021bda3cd87966d70f32017-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/518https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Species composition, susceptibility to antimicrobial agents, incidence and type of carbapenemase of carbapenemase-producing gram-negative bacteria, isolated in the Russian Research Centre of Radiology and Surgical Technologies from February 2014 to April 2016 were described. Screening of carbapenem-resistant bacteria was conducted by plating biomaterial on the chromogenic medium (“CHROMagar KPC”, DRG, France), species identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on the analyzer VITEK MS (bioMé- rieux, France). The sensitivity of gram-negative bacteria to antimicrobial drugs was determined on the analyzer VITEK-2 (bioMérieux), and the obtained results were interpreted in accordance with EUCAST criteria v.6.0, 2016. Genes encoding carbapenemase of KPC, OXA-48/162, VIM, IMP, NDM, OXA-51, OXA40/24, OXA-23 and OXA-58 groups were detected by multiplex real-time PCR (AmpliSens, Russia). 813 clinically relevant bacteria (602 patients) were obtained, including 405 gram-negative, among which 5.2% (21 strain from 16 patients) non-susceptible to meropenem and/or imipenem: Klebsiella pneumoniae (n = 5), Enterobacter cloacae (n = 2), Serratia marcescens (n = 1), Pseudomonas aeruginosa (n = 3), Acinetobacter baumannii (n = 10). Clinical material from 4 patients was infected by up to 3 carbapenem-non-susceptible strains of different species of gram-negative bacteria at the same time. The genes encoding acquired carbapenemases were detected in 84% of the nonsusceptible to carbapenems isolates: in A. baumannii — OXA40/24 (n = 8); in K. pneumoniae — OXA-48 (n = 1), KPC (n = 1) and NDM (n = 2); in P. aeruginosa — VIM (n = 1) and KPC (n = 1); in E. cloacae — KPC (n = 1). OXA-48 carbapenemase was also detected in one carbapenem-sensitive strain of K. pneumonia. All carbapenemase-producing strains had a phenotype of multidrug resistance. The results of the study showed that over the analyzed period, the prevalence of the carbapenem-nonsusceptible strains of gram-negative bacteria were relatively low, but all these strains were multidrug resistant and most of them possessed acquired carbapenemase genes. The species carbapenemnonsusceptible gram-negative bacteria and the types of the detected acquired carbapenemase were typical for Russia. For K. pneumoniae and P. aeruginosa no predominance of any one type of carbapenemase was revealed, while in all strains of A. baumannii the gene of OXA40/24 carbapenemase was found.A. G. PolischoukE. I. JakubovichO. V. PoluhinaV. V. OsovskichV. I. EvtushenkoSankt-Peterburg : NIIÈM imeni Pasteraarticlecarbapenemasesbacterial sensitivitygram-negative bacteriamaldi-tof msspecies identificationnosocomial infectionsInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 7, Iss 2, Pp 181-192 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	carbapenemases bacterial sensitivity gram-negative bacteria maldi-tof ms species identification nosocomial infections Infectious and parasitic diseases RC109-216
spellingShingle	carbapenemases bacterial sensitivity gram-negative bacteria maldi-tof ms species identification nosocomial infections Infectious and parasitic diseases RC109-216 A. G. Polischouk E. I. Jakubovich O. V. Poluhina V. V. Osovskich V. I. Evtushenko СARBAPENEMASE-PRODUCING GRAM-NEGATIVE BACTERIA IN A SPECIALIZED HOSPITAL OF ST. PETERSBURG
description	Species composition, susceptibility to antimicrobial agents, incidence and type of carbapenemase of carbapenemase-producing gram-negative bacteria, isolated in the Russian Research Centre of Radiology and Surgical Technologies from February 2014 to April 2016 were described. Screening of carbapenem-resistant bacteria was conducted by plating biomaterial on the chromogenic medium (“CHROMagar KPC”, DRG, France), species identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) on the analyzer VITEK MS (bioMé- rieux, France). The sensitivity of gram-negative bacteria to antimicrobial drugs was determined on the analyzer VITEK-2 (bioMérieux), and the obtained results were interpreted in accordance with EUCAST criteria v.6.0, 2016. Genes encoding carbapenemase of KPC, OXA-48/162, VIM, IMP, NDM, OXA-51, OXA40/24, OXA-23 and OXA-58 groups were detected by multiplex real-time PCR (AmpliSens, Russia). 813 clinically relevant bacteria (602 patients) were obtained, including 405 gram-negative, among which 5.2% (21 strain from 16 patients) non-susceptible to meropenem and/or imipenem: Klebsiella pneumoniae (n = 5), Enterobacter cloacae (n = 2), Serratia marcescens (n = 1), Pseudomonas aeruginosa (n = 3), Acinetobacter baumannii (n = 10). Clinical material from 4 patients was infected by up to 3 carbapenem-non-susceptible strains of different species of gram-negative bacteria at the same time. The genes encoding acquired carbapenemases were detected in 84% of the nonsusceptible to carbapenems isolates: in A. baumannii — OXA40/24 (n = 8); in K. pneumoniae — OXA-48 (n = 1), KPC (n = 1) and NDM (n = 2); in P. aeruginosa — VIM (n = 1) and KPC (n = 1); in E. cloacae — KPC (n = 1). OXA-48 carbapenemase was also detected in one carbapenem-sensitive strain of K. pneumonia. All carbapenemase-producing strains had a phenotype of multidrug resistance. The results of the study showed that over the analyzed period, the prevalence of the carbapenem-nonsusceptible strains of gram-negative bacteria were relatively low, but all these strains were multidrug resistant and most of them possessed acquired carbapenemase genes. The species carbapenemnonsusceptible gram-negative bacteria and the types of the detected acquired carbapenemase were typical for Russia. For K. pneumoniae and P. aeruginosa no predominance of any one type of carbapenemase was revealed, while in all strains of A. baumannii the gene of OXA40/24 carbapenemase was found.
format	article
author	A. G. Polischouk E. I. Jakubovich O. V. Poluhina V. V. Osovskich V. I. Evtushenko
author_facet	A. G. Polischouk E. I. Jakubovich O. V. Poluhina V. V. Osovskich V. I. Evtushenko
author_sort	A. G. Polischouk
title	СARBAPENEMASE-PRODUCING GRAM-NEGATIVE BACTERIA IN A SPECIALIZED HOSPITAL OF ST. PETERSBURG
title_short	СARBAPENEMASE-PRODUCING GRAM-NEGATIVE BACTERIA IN A SPECIALIZED HOSPITAL OF ST. PETERSBURG
title_full	СARBAPENEMASE-PRODUCING GRAM-NEGATIVE BACTERIA IN A SPECIALIZED HOSPITAL OF ST. PETERSBURG
title_fullStr	СARBAPENEMASE-PRODUCING GRAM-NEGATIVE BACTERIA IN A SPECIALIZED HOSPITAL OF ST. PETERSBURG
title_full_unstemmed	СARBAPENEMASE-PRODUCING GRAM-NEGATIVE BACTERIA IN A SPECIALIZED HOSPITAL OF ST. PETERSBURG
title_sort	сarbapenemase-producing gram-negative bacteria in a specialized hospital of st. petersburg
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2017
url	https://doaj.org/article/b1caa81b8f894021bda3cd87966d70f3
work_keys_str_mv	AT agpolischouk sarbapenemaseproducinggramnegativebacteriainaspecializedhospitalofstpetersburg AT eijakubovich sarbapenemaseproducinggramnegativebacteriainaspecializedhospitalofstpetersburg AT ovpoluhina sarbapenemaseproducinggramnegativebacteriainaspecializedhospitalofstpetersburg AT vvosovskich sarbapenemaseproducinggramnegativebacteriainaspecializedhospitalofstpetersburg AT vievtushenko sarbapenemaseproducinggramnegativebacteriainaspecializedhospitalofstpetersburg
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СARBAPENEMASE-PRODUCING GRAM-NEGATIVE BACTERIA IN A SPECIALIZED HOSPITAL OF ST. PETERSBURG

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