Sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.

<h4>Aims</h4>Mouse models of myocardial infarction (MI) are commonly used to explore the pathophysiological role of the monocytic response in myocardial injury and to develop translational strategies. However, no study thus far has examined the potential impact of inter-individual variab...

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Autores principales:	Jedrzej Hoffmann, Manuel Ospelt, Christian Troidl, Sandra Voss, Christoph Liebetrau, Won-Keun Kim, Andreas Rolf, Astrid Wietelmann, Thomas Braun, Kerstin Troidl, Sakthivel Sadayappan, David Barefield, Christian Hamm, Holger Nef, Helge Möllmann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1cd146186c84252aa1ee6581efc1812
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spelling	oai:doaj.org-article:b1cd146186c84252aa1ee6581efc18122021-11-18T08:17:19ZSham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.1932-620310.1371/journal.pone.0098456https://doaj.org/article/b1cd146186c84252aa1ee6581efc18122014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24893162/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Aims</h4>Mouse models of myocardial infarction (MI) are commonly used to explore the pathophysiological role of the monocytic response in myocardial injury and to develop translational strategies. However, no study thus far has examined the potential impact of inter-individual variability and sham surgical procedures on monocyte subset kinetics after experimental MI in mice. Our goal was to investigate determinants of systemic myeloid cell subset shifts in C57BL/6 mice following MI by developing a protocol for sequential extensive flow cytometry (FCM).<h4>Methods and results</h4>Following cross-sectional multiplex FCM analysis we provide for the first time a detailed description of absolute quantities, relative subset composition, and biological variability of circulating classical, intermediate, and non-classical monocyte subsets in C57BL/6 mice. By using intra-individual longitudinal measurements after MI induction, a time course of classical and non-classical monocytosis was recorded. This approach disclosed a significant reduction of monocyte subset dispersion across all investigated time points following MI. We found that in the current invasive model of chronic MI the global pattern of systemic monocyte kinetics is mainly determined by a nonspecific inflammatory response to sham surgery and not by the extent of myocardial injury.<h4>Conclusions</h4>Application of sequential multiplexed FCM may help to reduce the impact of biological variability in C57BL/6 mice. Furthermore, the confounding influence of sham surgical procedures should always be considered when measuring monocyte subset kinetics in a murine model of MI.Jedrzej HoffmannManuel OspeltChristian TroidlSandra VossChristoph LiebetrauWon-Keun KimAndreas RolfAstrid WietelmannThomas BraunKerstin TroidlSakthivel SadayappanDavid BarefieldChristian HammHolger NefHelge MöllmannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98456 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jedrzej Hoffmann Manuel Ospelt Christian Troidl Sandra Voss Christoph Liebetrau Won-Keun Kim Andreas Rolf Astrid Wietelmann Thomas Braun Kerstin Troidl Sakthivel Sadayappan David Barefield Christian Hamm Holger Nef Helge Möllmann Sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.
description	<h4>Aims</h4>Mouse models of myocardial infarction (MI) are commonly used to explore the pathophysiological role of the monocytic response in myocardial injury and to develop translational strategies. However, no study thus far has examined the potential impact of inter-individual variability and sham surgical procedures on monocyte subset kinetics after experimental MI in mice. Our goal was to investigate determinants of systemic myeloid cell subset shifts in C57BL/6 mice following MI by developing a protocol for sequential extensive flow cytometry (FCM).<h4>Methods and results</h4>Following cross-sectional multiplex FCM analysis we provide for the first time a detailed description of absolute quantities, relative subset composition, and biological variability of circulating classical, intermediate, and non-classical monocyte subsets in C57BL/6 mice. By using intra-individual longitudinal measurements after MI induction, a time course of classical and non-classical monocytosis was recorded. This approach disclosed a significant reduction of monocyte subset dispersion across all investigated time points following MI. We found that in the current invasive model of chronic MI the global pattern of systemic monocyte kinetics is mainly determined by a nonspecific inflammatory response to sham surgery and not by the extent of myocardial injury.<h4>Conclusions</h4>Application of sequential multiplexed FCM may help to reduce the impact of biological variability in C57BL/6 mice. Furthermore, the confounding influence of sham surgical procedures should always be considered when measuring monocyte subset kinetics in a murine model of MI.
format	article
author	Jedrzej Hoffmann Manuel Ospelt Christian Troidl Sandra Voss Christoph Liebetrau Won-Keun Kim Andreas Rolf Astrid Wietelmann Thomas Braun Kerstin Troidl Sakthivel Sadayappan David Barefield Christian Hamm Holger Nef Helge Möllmann
author_facet	Jedrzej Hoffmann Manuel Ospelt Christian Troidl Sandra Voss Christoph Liebetrau Won-Keun Kim Andreas Rolf Astrid Wietelmann Thomas Braun Kerstin Troidl Sakthivel Sadayappan David Barefield Christian Hamm Holger Nef Helge Möllmann
author_sort	Jedrzej Hoffmann
title	Sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.
title_short	Sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.
title_full	Sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.
title_fullStr	Sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.
title_full_unstemmed	Sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.
title_sort	sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b1cd146186c84252aa1ee6581efc1812
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Sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction.

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