Genetic Diversity Analysis and Barcoding in Tuberose (Polianthes tuberosa L.) Cultivars Using RAPD and ISSR Markers

Tuberose is one of the most important bulbous ornamentals grown commercially for loose as well as cut flowers. RAPD and ISSR markers used in the study revealed 53% and 73% polymorphism, respectively, among ten tuberose varieties. Polymorphic Information Content (PIC) and Resolving Power (RP) for RAP...

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Autores principales: K Khandagale, B Padmakar, D C Lakshmana Reddy, Anuradha Sane, C Aswath
Formato: article
Lenguaje:EN
Publicado: Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2014
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Acceso en línea:https://doaj.org/article/b1cdce2e08544d6d9da82de179350eb2
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spelling oai:doaj.org-article:b1cdce2e08544d6d9da82de179350eb22021-12-02T12:39:38ZGenetic Diversity Analysis and Barcoding in Tuberose (Polianthes tuberosa L.) Cultivars Using RAPD and ISSR Markers0973-354X2582-4899https://doaj.org/article/b1cdce2e08544d6d9da82de179350eb22014-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/209https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Tuberose is one of the most important bulbous ornamentals grown commercially for loose as well as cut flowers. RAPD and ISSR markers used in the study revealed 53% and 73% polymorphism, respectively, among ten tuberose varieties. Polymorphic Information Content (PIC) and Resolving Power (RP) for RAPD varied from 0.35 - 0.46 and 0.8 - 3.6, respectively, and that for ISSR was 0.36 - 0.49 and 0.91 - 4.55, respectively. The dendrogram (UPGMA), based on Jaccards co-efficient as similarity index for RAPD and ISSR, grouped ten varieties into two major clusters, and, combined RAPD-ISSR cluster analysis formed three major clusters based on their genetic relatedness/variation. PCA revealed that the spatial arrangement of these 10 cultivars was congruent with dendrogram analysis. Mantel's test indicated very good correlation, with r = 0.86 for combination of ISSR and RAPD-ISSR. To facilitate identification of tuberose cultivars, a cultivar identification diagram (CID) was developed in which seven ISSR loci could differentiate all the ten cultivars used in the study. Barcodes were developed for five cultivars released by IIHR using 57 polymorphic loci generated by 11 ISSR primers. The size of these loci ranged from 252bp to 2.2kb. These barcodes can be used as a standard reference source for quick identification of cultivars.K KhandagaleB PadmakarD C Lakshmana ReddyAnuradha SaneC AswathSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleissrmolecular barcodepcrrapdupgmaPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 9, Iss 1, Pp 5-11 (2014)
institution DOAJ
collection DOAJ
language EN
topic issr
molecular barcode
pcr
rapd
upgma
Plant culture
SB1-1110
spellingShingle issr
molecular barcode
pcr
rapd
upgma
Plant culture
SB1-1110
K Khandagale
B Padmakar
D C Lakshmana Reddy
Anuradha Sane
C Aswath
Genetic Diversity Analysis and Barcoding in Tuberose (Polianthes tuberosa L.) Cultivars Using RAPD and ISSR Markers
description Tuberose is one of the most important bulbous ornamentals grown commercially for loose as well as cut flowers. RAPD and ISSR markers used in the study revealed 53% and 73% polymorphism, respectively, among ten tuberose varieties. Polymorphic Information Content (PIC) and Resolving Power (RP) for RAPD varied from 0.35 - 0.46 and 0.8 - 3.6, respectively, and that for ISSR was 0.36 - 0.49 and 0.91 - 4.55, respectively. The dendrogram (UPGMA), based on Jaccards co-efficient as similarity index for RAPD and ISSR, grouped ten varieties into two major clusters, and, combined RAPD-ISSR cluster analysis formed three major clusters based on their genetic relatedness/variation. PCA revealed that the spatial arrangement of these 10 cultivars was congruent with dendrogram analysis. Mantel's test indicated very good correlation, with r = 0.86 for combination of ISSR and RAPD-ISSR. To facilitate identification of tuberose cultivars, a cultivar identification diagram (CID) was developed in which seven ISSR loci could differentiate all the ten cultivars used in the study. Barcodes were developed for five cultivars released by IIHR using 57 polymorphic loci generated by 11 ISSR primers. The size of these loci ranged from 252bp to 2.2kb. These barcodes can be used as a standard reference source for quick identification of cultivars.
format article
author K Khandagale
B Padmakar
D C Lakshmana Reddy
Anuradha Sane
C Aswath
author_facet K Khandagale
B Padmakar
D C Lakshmana Reddy
Anuradha Sane
C Aswath
author_sort K Khandagale
title Genetic Diversity Analysis and Barcoding in Tuberose (Polianthes tuberosa L.) Cultivars Using RAPD and ISSR Markers
title_short Genetic Diversity Analysis and Barcoding in Tuberose (Polianthes tuberosa L.) Cultivars Using RAPD and ISSR Markers
title_full Genetic Diversity Analysis and Barcoding in Tuberose (Polianthes tuberosa L.) Cultivars Using RAPD and ISSR Markers
title_fullStr Genetic Diversity Analysis and Barcoding in Tuberose (Polianthes tuberosa L.) Cultivars Using RAPD and ISSR Markers
title_full_unstemmed Genetic Diversity Analysis and Barcoding in Tuberose (Polianthes tuberosa L.) Cultivars Using RAPD and ISSR Markers
title_sort genetic diversity analysis and barcoding in tuberose (polianthes tuberosa l.) cultivars using rapd and issr markers
publisher Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate 2014
url https://doaj.org/article/b1cdce2e08544d6d9da82de179350eb2
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