The acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment

Mirko Resan,1,2 Miroslav Vukosavljevic,1,2 Danilo Vojvodic,2,3 Brigitte Pajic-Eggspuehler,4 Bojan Pajic2,4–6 1Eye Clinic, Military Medical Academy, Belgrade, Serbia; 2Faculty of Medicine of the Military Medical Academy, University of Defense, Belgrade, Serbia; 3Department for Clinical and...

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Autores principales:	Resan M, Vukosavljevic M, Vojvodic D, Pajic-Eggspuehler B, Pajic B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	LASIK PRK tears proinflammatory cytokines wound healing. Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/b1ce00a554784b31a688839ce9a1f215
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spelling	oai:doaj.org-article:b1ce00a554784b31a688839ce9a1f2152021-12-02T07:09:25ZThe acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment1177-5483https://doaj.org/article/b1ce00a554784b31a688839ce9a1f2152016-05-01T00:00:00Zhttps://www.dovepress.com/the-acute-phase-of-inflammatory-response-involved-in-the-wound-healing-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Mirko Resan,1,2 Miroslav Vukosavljevic,1,2 Danilo Vojvodic,2,3 Brigitte Pajic-Eggspuehler,4 Bojan Pajic2,4–6 1Eye Clinic, Military Medical Academy, Belgrade, Serbia; 2Faculty of Medicine of the Military Medical Academy, University of Defense, Belgrade, Serbia; 3Department for Clinical and Experimental Immunology, Institute for Medical Research, Military Medical Academy, Belgrade, Serbia; 4Eye Clinic ORASIS, Swiss Eye Research Foundation, Reinach AG, Switzerland; 5Department of Physics, Faculty of Science, University of Novi Sad, Novi Sad, Serbia; 6Division of Ophthalmology, Department of Clinical Neurosciences, University Hospitals of Geneva, Switzerland Purpose: To evaluate the participation of proinflammatory cytokines in the acute phase of corneal wound-healing response after excimer laser treatment. Methods: The study included 68 myopic eyes up to -3.0 diopters divided into two groups: 1) eyes treated with laser in situ keratomileusis (LASIK) (n=31) and 2) eyes treated with photorefractive keratectomy (PRK) (n=37). Each group was then divided into three subgroups based on tear sampling times: before (0 hours), 1 hour after, and 24 hours after treatment. The tear fluid was sampled from lower lateral tear meniscus using a cellulose microsurgical sponge. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8 in tear fluid were determined by flow cytometry method. Results: Statistical significance was observed in the concentrations of TNF-α (P=0.0421) and IL-1β (P=0.0225) between samples collected 1 and 24 hours after PRK treatment in favor of samples collected 1 hour after treatment. IL-6 concentration changes showed a significant increase in the PRK group in both time intervals following treatment compared to pretreatment (0 hour/1 hour, P=0.0031; 0 hour/24 hours, P=0.0059). For IL-8 concentrations, significant differences were observed between control and experimental groups in samples collected 1 hour after LASIK and 1 hour after PRK treatment (P<0.001 for both groups), and IL-8 concentrations between control and experimental groups in samples collected 24 hours after LASIK and 24 hours after PRK treatment were greater after PRK treatment (P=0.0005). Comparison of average concentration values of proinflammatory cytokines in all the tested samples between LASIK and PRK groups showed significantly higher levels of IL-1β in the LASIK group 24 hours after treatment (P=0.0134), and of IL-6 in the PRK group 24 hours after treatment (P=0.0031). Conclusion: The acute phase of corneal wound healing after excimer laser treatment is defined by an intensive inflammatory response. After PRK treatment, there were increased concentrations of TNF-α and IL-1β in tear samples 1 hour after treatment, IL-6, 1 and 24 hours after treatment, and IL-8, 1 and 24 hours after treatment. After LASIK treatment, there were increased concentrations of IL-8 in tear samples 1 hour after treatment and IL-1β, 24 hours after treatment. Both PRK and LASIK methods are characterized with a significant inflammatory response. However, tear findings following PRK method showed more intensive inflammatory response than the findings after LASIK method. Keywords: LASIK, PRK, tears, proinflammatory cytokines, wound healingResan MVukosavljevic MVojvodic DPajic-Eggspuehler BPajic BDove Medical PressarticleLASIKPRKtearsproinflammatory cytokineswound healing.OphthalmologyRE1-994ENClinical Ophthalmology, Vol 2016, Iss Issue 1, Pp 993-1000 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	LASIK PRK tears proinflammatory cytokines wound healing. Ophthalmology RE1-994
spellingShingle	LASIK PRK tears proinflammatory cytokines wound healing. Ophthalmology RE1-994 Resan M Vukosavljevic M Vojvodic D Pajic-Eggspuehler B Pajic B The acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment
description	Mirko Resan,1,2 Miroslav Vukosavljevic,1,2 Danilo Vojvodic,2,3 Brigitte Pajic-Eggspuehler,4 Bojan Pajic2,4–6 1Eye Clinic, Military Medical Academy, Belgrade, Serbia; 2Faculty of Medicine of the Military Medical Academy, University of Defense, Belgrade, Serbia; 3Department for Clinical and Experimental Immunology, Institute for Medical Research, Military Medical Academy, Belgrade, Serbia; 4Eye Clinic ORASIS, Swiss Eye Research Foundation, Reinach AG, Switzerland; 5Department of Physics, Faculty of Science, University of Novi Sad, Novi Sad, Serbia; 6Division of Ophthalmology, Department of Clinical Neurosciences, University Hospitals of Geneva, Switzerland Purpose: To evaluate the participation of proinflammatory cytokines in the acute phase of corneal wound-healing response after excimer laser treatment. Methods: The study included 68 myopic eyes up to -3.0 diopters divided into two groups: 1) eyes treated with laser in situ keratomileusis (LASIK) (n=31) and 2) eyes treated with photorefractive keratectomy (PRK) (n=37). Each group was then divided into three subgroups based on tear sampling times: before (0 hours), 1 hour after, and 24 hours after treatment. The tear fluid was sampled from lower lateral tear meniscus using a cellulose microsurgical sponge. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8 in tear fluid were determined by flow cytometry method. Results: Statistical significance was observed in the concentrations of TNF-α (P=0.0421) and IL-1β (P=0.0225) between samples collected 1 and 24 hours after PRK treatment in favor of samples collected 1 hour after treatment. IL-6 concentration changes showed a significant increase in the PRK group in both time intervals following treatment compared to pretreatment (0 hour/1 hour, P=0.0031; 0 hour/24 hours, P=0.0059). For IL-8 concentrations, significant differences were observed between control and experimental groups in samples collected 1 hour after LASIK and 1 hour after PRK treatment (P<0.001 for both groups), and IL-8 concentrations between control and experimental groups in samples collected 24 hours after LASIK and 24 hours after PRK treatment were greater after PRK treatment (P=0.0005). Comparison of average concentration values of proinflammatory cytokines in all the tested samples between LASIK and PRK groups showed significantly higher levels of IL-1β in the LASIK group 24 hours after treatment (P=0.0134), and of IL-6 in the PRK group 24 hours after treatment (P=0.0031). Conclusion: The acute phase of corneal wound healing after excimer laser treatment is defined by an intensive inflammatory response. After PRK treatment, there were increased concentrations of TNF-α and IL-1β in tear samples 1 hour after treatment, IL-6, 1 and 24 hours after treatment, and IL-8, 1 and 24 hours after treatment. After LASIK treatment, there were increased concentrations of IL-8 in tear samples 1 hour after treatment and IL-1β, 24 hours after treatment. Both PRK and LASIK methods are characterized with a significant inflammatory response. However, tear findings following PRK method showed more intensive inflammatory response than the findings after LASIK method. Keywords: LASIK, PRK, tears, proinflammatory cytokines, wound healing
format	article
author	Resan M Vukosavljevic M Vojvodic D Pajic-Eggspuehler B Pajic B
author_facet	Resan M Vukosavljevic M Vojvodic D Pajic-Eggspuehler B Pajic B
author_sort	Resan M
title	The acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment
title_short	The acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment
title_full	The acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment
title_fullStr	The acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment
title_full_unstemmed	The acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment
title_sort	acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/b1ce00a554784b31a688839ce9a1f215
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The acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment

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