Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing

CRISPR nucleases can be programmed to cleave sequences in specific bacteria to induce cell death. Here, Hamilton et al. present an optimized method for conjugative delivery of CRISPR nucleases, consisting of a single plasmid that encodes both the conjugative machinery and the nuclease.

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Autores principales:	Thomas A. Hamilton, Gregory M. Pellegrino, Jasmine A. Therrien, Dalton T. Ham, Peter C. Bartlett, Bogumil J. Karas, Gregory B. Gloor, David R. Edgell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b1cfdfe24838427d8b5b49368d6df85b
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spelling	oai:doaj.org-article:b1cfdfe24838427d8b5b49368d6df85b2021-12-02T16:57:30ZEfficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing10.1038/s41467-019-12448-32041-1723https://doaj.org/article/b1cfdfe24838427d8b5b49368d6df85b2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12448-3https://doaj.org/toc/2041-1723CRISPR nucleases can be programmed to cleave sequences in specific bacteria to induce cell death. Here, Hamilton et al. present an optimized method for conjugative delivery of CRISPR nucleases, consisting of a single plasmid that encodes both the conjugative machinery and the nuclease.Thomas A. HamiltonGregory M. PellegrinoJasmine A. TherrienDalton T. HamPeter C. BartlettBogumil J. KarasGregory B. GloorDavid R. EdgellNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Thomas A. Hamilton Gregory M. Pellegrino Jasmine A. Therrien Dalton T. Ham Peter C. Bartlett Bogumil J. Karas Gregory B. Gloor David R. Edgell Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing
description	CRISPR nucleases can be programmed to cleave sequences in specific bacteria to induce cell death. Here, Hamilton et al. present an optimized method for conjugative delivery of CRISPR nucleases, consisting of a single plasmid that encodes both the conjugative machinery and the nuclease.
format	article
author	Thomas A. Hamilton Gregory M. Pellegrino Jasmine A. Therrien Dalton T. Ham Peter C. Bartlett Bogumil J. Karas Gregory B. Gloor David R. Edgell
author_facet	Thomas A. Hamilton Gregory M. Pellegrino Jasmine A. Therrien Dalton T. Ham Peter C. Bartlett Bogumil J. Karas Gregory B. Gloor David R. Edgell
author_sort	Thomas A. Hamilton
title	Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing
title_short	Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing
title_full	Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing
title_fullStr	Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing
title_full_unstemmed	Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing
title_sort	efficient inter-species conjugative transfer of a crispr nuclease for targeted bacterial killing
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/b1cfdfe24838427d8b5b49368d6df85b
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Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing

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