How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?

Environmental conditions, such as nutrient concentrations, salinity, elevation etc., shape diatom assemblages of periphytic biofilms. These assemblages respond rapidly to environmental changes, a fact which makes diatoms valuable bioindicators. Hence, freshwater biomonitoring programmes currently us...

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Autores principales:	Panayiota Pissaridou, Marco Cantonati, Agnes Bouchez, Iakovos Tziortzis, Gerald Dörflinger, Marlen I. Vasquez
Formato:	article
Lenguaje:	EN
Publicado:	Pensoft Publishers 2021
Materias:	Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/b1d17e989a634d9fa7e5e6ad60ccd971
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spelling	oai:doaj.org-article:b1d17e989a634d9fa7e5e6ad60ccd9712021-12-02T18:09:46ZHow can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?10.3897/mbmg.5.684382534-9708https://doaj.org/article/b1d17e989a634d9fa7e5e6ad60ccd9712021-08-01T00:00:00Zhttps://mbmg.pensoft.net/article/68438/download/pdf/https://mbmg.pensoft.net/article/68438/download/xml/https://mbmg.pensoft.net/article/68438/https://doaj.org/toc/2534-9708Environmental conditions, such as nutrient concentrations, salinity, elevation etc., shape diatom assemblages of periphytic biofilms. These assemblages respond rapidly to environmental changes, a fact which makes diatoms valuable bioindicators. Hence, freshwater biomonitoring programmes currently use diatom indices (e.g. EU Water Framework Directive - WFD). To date, microscopy-based assessments require high taxonomic expertise for diatom identification at the species level. High-throughput technologies now provide cost-effective identification approaches that are promising, complementary or alternative tools for bioassessment. The suitability of the metabarcoding method is evaluated for the first time in the Cyprus streams WFD monitoring network, an eastern Mediterranean country with many endemic species and results are compared to the results acquired from the morphotaxonomic analysis. Morphotaxonomic identification was conducted microscopically, using the most updated taxonomic concepts, literature and online resources. At the same time, DNA metabarcoding involved the use of the rbcL 312 bp barcode, high-throughput sequencing and bioinformatic analysis. The ecological status was calculated using the IPS Index. Results show a positive correlation between morpho-taxonomic and molecular IPS scores. Discrepancies between the two methodologies are related to the limitations of both techniques. This study confirmed that Fistulifera saprophila can have a crucial role in key differences observed, as it negatively influences IPS scores and microscopy methods frequently overlook it. Importantly, gaps in the DNA barcoding reference databases lead to a positive overestimation in IPS scores. Overall, we conclude that DNA metabarcoding offsets the morphotaxonomic methodology for the ecological quality assessment of freshwaters.Panayiota PissaridouMarco CantonatiAgnes BouchezIakovos TziortzisGerald DörflingerMarlen I. VasquezPensoft PublishersarticleEcologyQH540-549.5ENMetabarcoding and Metagenomics, Vol 5, Iss , Pp 111-120 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ecology QH540-549.5
spellingShingle	Ecology QH540-549.5 Panayiota Pissaridou Marco Cantonati Agnes Bouchez Iakovos Tziortzis Gerald Dörflinger Marlen I. Vasquez How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?
description	Environmental conditions, such as nutrient concentrations, salinity, elevation etc., shape diatom assemblages of periphytic biofilms. These assemblages respond rapidly to environmental changes, a fact which makes diatoms valuable bioindicators. Hence, freshwater biomonitoring programmes currently use diatom indices (e.g. EU Water Framework Directive - WFD). To date, microscopy-based assessments require high taxonomic expertise for diatom identification at the species level. High-throughput technologies now provide cost-effective identification approaches that are promising, complementary or alternative tools for bioassessment. The suitability of the metabarcoding method is evaluated for the first time in the Cyprus streams WFD monitoring network, an eastern Mediterranean country with many endemic species and results are compared to the results acquired from the morphotaxonomic analysis. Morphotaxonomic identification was conducted microscopically, using the most updated taxonomic concepts, literature and online resources. At the same time, DNA metabarcoding involved the use of the rbcL 312 bp barcode, high-throughput sequencing and bioinformatic analysis. The ecological status was calculated using the IPS Index. Results show a positive correlation between morpho-taxonomic and molecular IPS scores. Discrepancies between the two methodologies are related to the limitations of both techniques. This study confirmed that Fistulifera saprophila can have a crucial role in key differences observed, as it negatively influences IPS scores and microscopy methods frequently overlook it. Importantly, gaps in the DNA barcoding reference databases lead to a positive overestimation in IPS scores. Overall, we conclude that DNA metabarcoding offsets the morphotaxonomic methodology for the ecological quality assessment of freshwaters.
format	article
author	Panayiota Pissaridou Marco Cantonati Agnes Bouchez Iakovos Tziortzis Gerald Dörflinger Marlen I. Vasquez
author_facet	Panayiota Pissaridou Marco Cantonati Agnes Bouchez Iakovos Tziortzis Gerald Dörflinger Marlen I. Vasquez
author_sort	Panayiota Pissaridou
title	How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?
title_short	How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?
title_full	How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?
title_fullStr	How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?
title_full_unstemmed	How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?
title_sort	how can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?
publisher	Pensoft Publishers
publishDate	2021
url	https://doaj.org/article/b1d17e989a634d9fa7e5e6ad60ccd971
work_keys_str_mv	AT panayiotapissaridou howcanintegratedmorphotaxonomyandmetabarcodingbaseddiatomassemblageanalysesbestcontributetotheecologicalassessmentofstreams AT marcocantonati howcanintegratedmorphotaxonomyandmetabarcodingbaseddiatomassemblageanalysesbestcontributetotheecologicalassessmentofstreams AT agnesbouchez howcanintegratedmorphotaxonomyandmetabarcodingbaseddiatomassemblageanalysesbestcontributetotheecologicalassessmentofstreams AT iakovostziortzis howcanintegratedmorphotaxonomyandmetabarcodingbaseddiatomassemblageanalysesbestcontributetotheecologicalassessmentofstreams AT geralddorflinger howcanintegratedmorphotaxonomyandmetabarcodingbaseddiatomassemblageanalysesbestcontributetotheecologicalassessmentofstreams AT marlenivasquez howcanintegratedmorphotaxonomyandmetabarcodingbaseddiatomassemblageanalysesbestcontributetotheecologicalassessmentofstreams
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How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?

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