Non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.

<h4>Background</h4>Molecular imaging of lung diseases, including asthma, is limited and either invasive or non-specific. Central to the inflammatory process in asthma is the recruitment of eosinophils to the airways, which release proteases and proinflammatory factors and contribute to a...

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Autores principales:	M Andrea Markus, Christian Dullin, Miso Mitkovski, Eva Prieschl-Grassauer, Michelle M Epstein, Frauke Alves
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b1d66c9732f54d6da4343751eb1ac54c
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spelling	oai:doaj.org-article:b1d66c9732f54d6da4343751eb1ac54c2021-11-18T08:31:03ZNon-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.1932-620310.1371/journal.pone.0090017https://doaj.org/article/b1d66c9732f54d6da4343751eb1ac54c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24587190/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Molecular imaging of lung diseases, including asthma, is limited and either invasive or non-specific. Central to the inflammatory process in asthma is the recruitment of eosinophils to the airways, which release proteases and proinflammatory factors and contribute to airway remodeling. The aim of this study was to establish a new approach to non-invasively assess lung eosinophilia during the course of experimental asthma by combining non-invasive near-infrared fluorescence (NIRF) imaging with the specific detection of Siglec-F, a lectin found predominantly on eosinophils.<h4>Methodology/principal findings</h4>An ovalbumin (OVA)-based model was used to induce asthma-like experimental allergic airway disease (EAAD) in BALB/c mice. By means of a NIRF imager, we demonstrate that 48 h-72 h after intravenous (i.v.) application of a NIRF-labeled anti-Siglec-F antibody, mice with EAAD exhibited up to 2 times higher fluorescence intensities compared to lungs of control mice. Furthermore, average lung intensities of dexamethasone-treated as well as beta-escin-treated mice were 1.8 and 2 times lower than those of untreated, EAAD mice, respectively and correlated with the reduction of cell infiltration in the lung. Average fluorescence intensities measured in explanted lungs confirmed the in vivo findings of significantly higher values in inflamed lungs as compared to controls. Fluorescence microscopy of lung cryosections localized the i.v. applied NIRF-labeled anti-Siglec-F antibody predominantly to eosinophils in the peribronchial areas of EAAD lungs as opposed to control lungs.<h4>Conclusion/significance</h4>We show that monitoring the occurrence of eosinophils, a prominent feature of allergic asthma, by means of a NIRF-labeled antibody directed against Siglec-F is a novel and powerful non-invasive optical imaging approach to assess EAAD and therapeutic response in mice over time.M Andrea MarkusChristian DullinMiso MitkovskiEva Prieschl-GrassauerMichelle M EpsteinFrauke AlvesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e90017 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M Andrea Markus Christian Dullin Miso Mitkovski Eva Prieschl-Grassauer Michelle M Epstein Frauke Alves Non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.
description	<h4>Background</h4>Molecular imaging of lung diseases, including asthma, is limited and either invasive or non-specific. Central to the inflammatory process in asthma is the recruitment of eosinophils to the airways, which release proteases and proinflammatory factors and contribute to airway remodeling. The aim of this study was to establish a new approach to non-invasively assess lung eosinophilia during the course of experimental asthma by combining non-invasive near-infrared fluorescence (NIRF) imaging with the specific detection of Siglec-F, a lectin found predominantly on eosinophils.<h4>Methodology/principal findings</h4>An ovalbumin (OVA)-based model was used to induce asthma-like experimental allergic airway disease (EAAD) in BALB/c mice. By means of a NIRF imager, we demonstrate that 48 h-72 h after intravenous (i.v.) application of a NIRF-labeled anti-Siglec-F antibody, mice with EAAD exhibited up to 2 times higher fluorescence intensities compared to lungs of control mice. Furthermore, average lung intensities of dexamethasone-treated as well as beta-escin-treated mice were 1.8 and 2 times lower than those of untreated, EAAD mice, respectively and correlated with the reduction of cell infiltration in the lung. Average fluorescence intensities measured in explanted lungs confirmed the in vivo findings of significantly higher values in inflamed lungs as compared to controls. Fluorescence microscopy of lung cryosections localized the i.v. applied NIRF-labeled anti-Siglec-F antibody predominantly to eosinophils in the peribronchial areas of EAAD lungs as opposed to control lungs.<h4>Conclusion/significance</h4>We show that monitoring the occurrence of eosinophils, a prominent feature of allergic asthma, by means of a NIRF-labeled antibody directed against Siglec-F is a novel and powerful non-invasive optical imaging approach to assess EAAD and therapeutic response in mice over time.
format	article
author	M Andrea Markus Christian Dullin Miso Mitkovski Eva Prieschl-Grassauer Michelle M Epstein Frauke Alves
author_facet	M Andrea Markus Christian Dullin Miso Mitkovski Eva Prieschl-Grassauer Michelle M Epstein Frauke Alves
author_sort	M Andrea Markus
title	Non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.
title_short	Non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.
title_full	Non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.
title_fullStr	Non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.
title_full_unstemmed	Non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.
title_sort	non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b1d66c9732f54d6da4343751eb1ac54c
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Non-invasive optical imaging of eosinophilia during the course of an experimental allergic airways disease model and in response to therapy.

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